Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice

Detalhes bibliográficos
Autor(a) principal: Loyo Casao, Thalia del Rosario
Data de Publicação: 2020
Outros Autores: Pinheiro, Camila Graca, Sarandy, Mariaurea Matias, Zanatta, Ana Caroline [UNESP], Vilegas, Wagner [UNESP], Novaes, Romulo Dias, Goncalves, Reggiani Vilela, Leite, Joao Paulo Viana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jep.2020.113042
http://hdl.handle.net/11449/197282
Resumo: Ethnopharmacological relevance: Croton urucurana Baill. (Euphorbiaceae) is a plant used in Brazilian popular medicine for the treatment of wound healing, inflammatory diseases, gastritis, infections, and hemorrhoids. Aim: The present study aimed to evaluate the in vivo wound healing activity of an ointment based on ethanolic extract of C. urucurana stem bark, at concentrations of 5% and 10%, and to relate it with compounds that could be associated with this activity. Materials and methods: Analyses by FIA-ESI-IT-MS were carried out to investigate the chemical composition of C. urucurana. Knockout IL-10 (n = 60) mice and wild type C57 (n = 12) mice were separated into 6 groups to evaluate the wound healing activity. Knockout IL-10 mice: SAL (0.9% saline); BAS (ointment base); SS (1% silver sulfadiazine); CR1 (ointment with extract of C. urucurana 5%); CR2 (ointment with extract of C. urucurana 10%); and wild mice C57: SALC57 (Saline 0.9%). A circular wound with 10 mm in diameter was generated on the dorsal of the animals. Tissue specimen of the wounds were removed on days 7 and 14 of the treatment for histopathological, oxidative status and analyses of pro-and anti-inflammatory cytokines in scar tissue. Results: In the phytochemical profile, twelve proanthocyanidins were identified (in the form of monomers, dimers, trimers, and tetramers), based on (epi)catechin and (epi)gallocatechin. Furthermore, two quercetin derivatives and two alkaloids were detected. The groups treated with CR1 and CR2 ointments presented higher rate of wound closure, increased total number of cells, mast cells, blood vessels and higher deposition of type III and I collagen. In addition, they showed increased amount of pro-inflammatory cytokines (IL- 2 and IFN-gamma), and anti-inflmatory cytokines (IL-4), on the 7th day of treatment. Conclusion: The results presented support the popular use of preparations based on the bark of C. urucurana as a healing compound.
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spelling Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 miceCroton urucuranaMedicinal plantWound healingOxidative stressProanthocyanidinsEthnopharmacological relevance: Croton urucurana Baill. (Euphorbiaceae) is a plant used in Brazilian popular medicine for the treatment of wound healing, inflammatory diseases, gastritis, infections, and hemorrhoids. Aim: The present study aimed to evaluate the in vivo wound healing activity of an ointment based on ethanolic extract of C. urucurana stem bark, at concentrations of 5% and 10%, and to relate it with compounds that could be associated with this activity. Materials and methods: Analyses by FIA-ESI-IT-MS were carried out to investigate the chemical composition of C. urucurana. Knockout IL-10 (n = 60) mice and wild type C57 (n = 12) mice were separated into 6 groups to evaluate the wound healing activity. Knockout IL-10 mice: SAL (0.9% saline); BAS (ointment base); SS (1% silver sulfadiazine); CR1 (ointment with extract of C. urucurana 5%); CR2 (ointment with extract of C. urucurana 10%); and wild mice C57: SALC57 (Saline 0.9%). A circular wound with 10 mm in diameter was generated on the dorsal of the animals. Tissue specimen of the wounds were removed on days 7 and 14 of the treatment for histopathological, oxidative status and analyses of pro-and anti-inflammatory cytokines in scar tissue. Results: In the phytochemical profile, twelve proanthocyanidins were identified (in the form of monomers, dimers, trimers, and tetramers), based on (epi)catechin and (epi)gallocatechin. Furthermore, two quercetin derivatives and two alkaloids were detected. The groups treated with CR1 and CR2 ointments presented higher rate of wound closure, increased total number of cells, mast cells, blood vessels and higher deposition of type III and I collagen. In addition, they showed increased amount of pro-inflammatory cytokines (IL- 2 and IFN-gamma), and anti-inflmatory cytokines (IL-4), on the 7th day of treatment. Conclusion: The results presented support the popular use of preparations based on the bark of C. urucurana as a healing compound.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Vicosa Fed Univ, Dept Biochem & Mol Biol, BR-35570900 Vicosa, MG, BrazilVicosa Fed Univ, Dept Anim Biol, BR-35570900 Vicosa, MG, BrazilSao Paulo State Univ, Inst Chem, BR-14800900 Araraquara, SP, BrazilSao Paulo State Univ, Inst Biosci, BR-05508900 Sao Vicente, SP, BrazilUniv Fed Alfenas, Dept Struct Biol, BR-37130001 Alfenas, MG, BrazilSao Paulo State Univ, Inst Chem, BR-14800900 Araraquara, SP, BrazilSao Paulo State Univ, Inst Biosci, BR-05508900 Sao Vicente, SP, BrazilFAPEMIG: APQ-01895-16FAPEMIG: PPM-00687-17FAPEMIG: PPM-00077-18CNPq: 303972/2017-3CNPq: 423594/2018-4CNPq: 305093/2017-7CNPq: MCTIC 408503/2018-1CAPES: 001Elsevier B.V.Vicosa Fed UnivUniversidade Estadual Paulista (Unesp)Univ Fed AlfenasLoyo Casao, Thalia del RosarioPinheiro, Camila GracaSarandy, Mariaurea MatiasZanatta, Ana Caroline [UNESP]Vilegas, Wagner [UNESP]Novaes, Romulo DiasGoncalves, Reggiani VilelaLeite, Joao Paulo Viana2020-12-10T20:12:01Z2020-12-10T20:12:01Z2020-10-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1016/j.jep.2020.113042Journal Of Ethnopharmacology. Clare: Elsevier Ireland Ltd, v. 261, 12 p., 2020.0378-8741http://hdl.handle.net/11449/19728210.1016/j.jep.2020.113042WOS:000566341400004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Ethnopharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T12:31:47Zoai:repositorio.unesp.br:11449/197282Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:30:17.014793Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
title Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
spellingShingle Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
Loyo Casao, Thalia del Rosario
Croton urucurana
Medicinal plant
Wound healing
Oxidative stress
Proanthocyanidins
title_short Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
title_full Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
title_fullStr Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
title_full_unstemmed Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
title_sort Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice
author Loyo Casao, Thalia del Rosario
author_facet Loyo Casao, Thalia del Rosario
Pinheiro, Camila Graca
Sarandy, Mariaurea Matias
Zanatta, Ana Caroline [UNESP]
Vilegas, Wagner [UNESP]
Novaes, Romulo Dias
Goncalves, Reggiani Vilela
Leite, Joao Paulo Viana
author_role author
author2 Pinheiro, Camila Graca
Sarandy, Mariaurea Matias
Zanatta, Ana Caroline [UNESP]
Vilegas, Wagner [UNESP]
Novaes, Romulo Dias
Goncalves, Reggiani Vilela
Leite, Joao Paulo Viana
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Vicosa Fed Univ
Universidade Estadual Paulista (Unesp)
Univ Fed Alfenas
dc.contributor.author.fl_str_mv Loyo Casao, Thalia del Rosario
Pinheiro, Camila Graca
Sarandy, Mariaurea Matias
Zanatta, Ana Caroline [UNESP]
Vilegas, Wagner [UNESP]
Novaes, Romulo Dias
Goncalves, Reggiani Vilela
Leite, Joao Paulo Viana
dc.subject.por.fl_str_mv Croton urucurana
Medicinal plant
Wound healing
Oxidative stress
Proanthocyanidins
topic Croton urucurana
Medicinal plant
Wound healing
Oxidative stress
Proanthocyanidins
description Ethnopharmacological relevance: Croton urucurana Baill. (Euphorbiaceae) is a plant used in Brazilian popular medicine for the treatment of wound healing, inflammatory diseases, gastritis, infections, and hemorrhoids. Aim: The present study aimed to evaluate the in vivo wound healing activity of an ointment based on ethanolic extract of C. urucurana stem bark, at concentrations of 5% and 10%, and to relate it with compounds that could be associated with this activity. Materials and methods: Analyses by FIA-ESI-IT-MS were carried out to investigate the chemical composition of C. urucurana. Knockout IL-10 (n = 60) mice and wild type C57 (n = 12) mice were separated into 6 groups to evaluate the wound healing activity. Knockout IL-10 mice: SAL (0.9% saline); BAS (ointment base); SS (1% silver sulfadiazine); CR1 (ointment with extract of C. urucurana 5%); CR2 (ointment with extract of C. urucurana 10%); and wild mice C57: SALC57 (Saline 0.9%). A circular wound with 10 mm in diameter was generated on the dorsal of the animals. Tissue specimen of the wounds were removed on days 7 and 14 of the treatment for histopathological, oxidative status and analyses of pro-and anti-inflammatory cytokines in scar tissue. Results: In the phytochemical profile, twelve proanthocyanidins were identified (in the form of monomers, dimers, trimers, and tetramers), based on (epi)catechin and (epi)gallocatechin. Furthermore, two quercetin derivatives and two alkaloids were detected. The groups treated with CR1 and CR2 ointments presented higher rate of wound closure, increased total number of cells, mast cells, blood vessels and higher deposition of type III and I collagen. In addition, they showed increased amount of pro-inflammatory cytokines (IL- 2 and IFN-gamma), and anti-inflmatory cytokines (IL-4), on the 7th day of treatment. Conclusion: The results presented support the popular use of preparations based on the bark of C. urucurana as a healing compound.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T20:12:01Z
2020-12-10T20:12:01Z
2020-10-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jep.2020.113042
Journal Of Ethnopharmacology. Clare: Elsevier Ireland Ltd, v. 261, 12 p., 2020.
0378-8741
http://hdl.handle.net/11449/197282
10.1016/j.jep.2020.113042
WOS:000566341400004
url http://dx.doi.org/10.1016/j.jep.2020.113042
http://hdl.handle.net/11449/197282
identifier_str_mv Journal Of Ethnopharmacology. Clare: Elsevier Ireland Ltd, v. 261, 12 p., 2020.
0378-8741
10.1016/j.jep.2020.113042
WOS:000566341400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Ethnopharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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