Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0079971 http://hdl.handle.net/11449/112708 |
Resumo: | Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure and multiple organ failure that can lead to death. Previous attempts to develop specific antivenom to treat these victims have been unsuccessful. We herein report a F(ab)'(2)-based antivenom raised in horse as a potential new treatment for victims of multiple bee stings. The final product contains high specific IgG titers and is effective in neutralizing toxic effects, such as hemolysis, cytotoxicity and myotoxicity. The assessment of neutralization was revised and hemolysis, the primary toxic effect of these stings, was fully neutralized in vivo for the first time. |
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Repositório Institucional da UNESP |
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Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized BeesVictims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure and multiple organ failure that can lead to death. Previous attempts to develop specific antivenom to treat these victims have been unsuccessful. We herein report a F(ab)'(2)-based antivenom raised in horse as a potential new treatment for victims of multiple bee stings. The final product contains high specific IgG titers and is effective in neutralizing toxic effects, such as hemolysis, cytotoxicity and myotoxicity. The assessment of neutralization was revised and hemolysis, the primary toxic effect of these stings, was fully neutralized in vivo for the first time.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FINEP (CT-BIOTEC-Anticorpos -MCTFINEP)Butantan InstituteCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Sch Med, FMUSP, Div Clin Immunol & Allergy, Sao Paulo, BrazilInst Invest Immunol INCT, Sao Paulo, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo, BrazilButantan Inst, Div Hyperimmune Plasmas, Sao Paulo, BrazilUniv Sao Francisco, Sch Med, BR-12900000 Braganca Paulista, SP, BrazilButantan Inst, Special Pilot Lab Res & Dev Vet Immunobiol, Sao Paulo, BrazilInst Technol Parana, Curitiba, Parana, BrazilButantan Inst, Lab Biopharmaceut Anim Cells, Sao Paulo, BrazilUniv Sao Paulo, Sch Med, Heart Inst InCor, LIM 19, Sao Paulo, BrazilUniv Sao Paulo State UNESP, Ctr Study Social Insects, Inst Biosci, Dept Biol, Rio Claro, SP, BrazilUniv Sao Paulo State UNESP, Ctr Study Social Insects, Inst Biosci, Dept Biol, Rio Claro, SP, BrazilFAPESP: 11/51684-1Public Library ScienceUniversidade de São Paulo (USP)Inst Invest Immunol INCTInstituto ButantanUniv Sao FranciscoInst Technol ParanaUniversidade Estadual Paulista (Unesp)Santos, Keity SouzaStephano, Marco AntonioMarcelino, Jose RobertoResende Ferreira, Virginia MariaRocha, ThalitaCaricati, CelsoHigashi, Hisako GondoMoro, Ana MariaKalil, Jorge EliasMalaspina, Osmar [UNESP]Morato Castro, Fabio FernandesPalma, Mario Sergio [UNESP]2014-12-03T13:11:00Z2014-12-03T13:11:00Z2013-11-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1371/journal.pone.0079971Plos One. San Francisco: Public Library Science, v. 8, n. 11, 10 p., 2013.1932-6203http://hdl.handle.net/11449/11270810.1371/journal.pone.0079971WOS:000327254700167WOS000327254700167.pdf753855608550581929018886245065350000-0002-1650-257XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-11-01T06:09:24Zoai:repositorio.unesp.br:11449/112708Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:35:52.650173Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees |
title |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees |
spellingShingle |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees Santos, Keity Souza |
title_short |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees |
title_full |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees |
title_fullStr |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees |
title_full_unstemmed |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees |
title_sort |
Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees |
author |
Santos, Keity Souza |
author_facet |
Santos, Keity Souza Stephano, Marco Antonio Marcelino, Jose Roberto Resende Ferreira, Virginia Maria Rocha, Thalita Caricati, Celso Higashi, Hisako Gondo Moro, Ana Maria Kalil, Jorge Elias Malaspina, Osmar [UNESP] Morato Castro, Fabio Fernandes Palma, Mario Sergio [UNESP] |
author_role |
author |
author2 |
Stephano, Marco Antonio Marcelino, Jose Roberto Resende Ferreira, Virginia Maria Rocha, Thalita Caricati, Celso Higashi, Hisako Gondo Moro, Ana Maria Kalil, Jorge Elias Malaspina, Osmar [UNESP] Morato Castro, Fabio Fernandes Palma, Mario Sergio [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Inst Invest Immunol INCT Instituto Butantan Univ Sao Francisco Inst Technol Parana Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santos, Keity Souza Stephano, Marco Antonio Marcelino, Jose Roberto Resende Ferreira, Virginia Maria Rocha, Thalita Caricati, Celso Higashi, Hisako Gondo Moro, Ana Maria Kalil, Jorge Elias Malaspina, Osmar [UNESP] Morato Castro, Fabio Fernandes Palma, Mario Sergio [UNESP] |
description |
Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure and multiple organ failure that can lead to death. Previous attempts to develop specific antivenom to treat these victims have been unsuccessful. We herein report a F(ab)'(2)-based antivenom raised in horse as a potential new treatment for victims of multiple bee stings. The final product contains high specific IgG titers and is effective in neutralizing toxic effects, such as hemolysis, cytotoxicity and myotoxicity. The assessment of neutralization was revised and hemolysis, the primary toxic effect of these stings, was fully neutralized in vivo for the first time. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-13 2014-12-03T13:11:00Z 2014-12-03T13:11:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0079971 Plos One. San Francisco: Public Library Science, v. 8, n. 11, 10 p., 2013. 1932-6203 http://hdl.handle.net/11449/112708 10.1371/journal.pone.0079971 WOS:000327254700167 WOS000327254700167.pdf 7538556085505819 2901888624506535 0000-0002-1650-257X |
url |
http://dx.doi.org/10.1371/journal.pone.0079971 http://hdl.handle.net/11449/112708 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 8, n. 11, 10 p., 2013. 1932-6203 10.1371/journal.pone.0079971 WOS:000327254700167 WOS000327254700167.pdf 7538556085505819 2901888624506535 0000-0002-1650-257X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128675927818240 |