DETERMINATION OF MERCURY IN LIVER OF MARINE TETRAPODS BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROMETRY AND INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY IN BIOLOGICAL SAMPLES: A SYSTEMATIC COMPARISON BETWEEN THE TWO TECHNIQUES

• Autor(a) principal: Silva, Raiza F. da [UNESP]
• Data de Publicação: 2021
• Outros Autores: Rocha, Siomara D. da, Menegario, Amauri A. [UNESP], Pedrobom, Jorge H. [UNESP], Sulato, Everton T. [UNESP], Luko, Karen S. [UNESP], Elias, Lucas Pellegrini [UNESP], Oliveira, Lucia M. de Souza [UNESP], Sargentini Junior, Ezio
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.21577/0100-4042.20170675; http://hdl.handle.net/11449/210144
• topic: Mercury; Marine Tetrapod; Hg Memory Effect; ICP-MS; CV-AFS

This study compares the techniques cold vapour atomic fluorescence spectrometry (CV-AFS) and inductively coupled plasma mass spectrometry (ICP-MS) for the determination of Hg in biological samples (hepatic tissue from marine tetrapods). Acid digestions were performed for the samples and aliquots of them were analysed by ICP-MS and CV-AFS. To minimize the Hg memory effect in its determination by ICP-MS, gold (200 mu g L-1 in 5% v v(-1) HCl) was added to the sample digests. The detection limits were 0.02 mg kg(-1) for ICP-MS and 0.01 mg kg(-1) for CV-AFS. Accuracy was assessed by the calculation of the recovery obtained from TORT-3 (lobster hepatopancreas) certified reference material analysis. Accurate results were obtained for both CV-AFS and ICP-MS. The comparison of the results obtained for hepatic tissue with and without lyophilization showed a significant difference among them (at 95% of confidence level), evidencing losses of Hg in the lyophilization process.