Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pharmaceutics15051474 http://hdl.handle.net/11449/249997 |
Resumo: | The application of mesenchymal stem cells (MSC) in bone tissue regeneration can have unpredictable results due to the low survival of cells in the process since the lack of oxygen and nutrients promotes metabolic stress. Therefore, in this work, polymeric membranes formed by organic–inorganic hybrid materials called ureasil-polyether for modified glucose release were developed in order to overcome the problems posed by a of lack of this nutrient. Thus, membranes formed by polymeric blend of polypropylene oxide (PPO4000) and polyethylene oxide (PEO500) with 6% glucose incorporation were developed. Physical–chemical characterization techniques were performed, as well as tests that evaluated thermal properties, bioactivity, swelling, and release in SBF solution. The results of the swelling test showed an increase in membrane mass correlated with an increase in the concentration of ureasil-PEO500 in the polymeric blends. The membranes showed adequate resistance when subjected to the application of a high compression force (15 N). X-ray diffraction (XRD) evidenced peaks corresponding to orthorhombic crystalline organization, but the absence of glucose-related peaks showed characteristics of the amorphous regions of hybrid materials, likely due to solubilization. Thermogravimetry (TG) and differential scanning calorimetry (DSC) analyses showed that the thermal events attributed to glucose and hybrid materials were similar to that seen in the literature, however when glucose was incorporated into the PEO500, an increase in rigidity occurs. In PPO400, and in the blends of both materials, there was a slight decrease in Tg values. The smaller contact angle for the ureasil-PEO500 membrane revealed the more hydrophilic character of the material compared to other membranes. The membranes showed bioactivity and hemocompatibility in vitro. The in vitro release test revealed that it is possible to control the release rate of glucose and the kinetic analysis revealed a release mechanism characteristic of anomalous transport kinetics. Thus, we can conclude that ureasil-polyether membranes have great potential to be used as a glucose release system, and their future application has the potential to optimize the bone regeneration process. |
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Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regenerationbone regenerationglucose releaseorganic–inorganic hybridspolyethylene oxidepolypropylene oxidesol–gel processThe application of mesenchymal stem cells (MSC) in bone tissue regeneration can have unpredictable results due to the low survival of cells in the process since the lack of oxygen and nutrients promotes metabolic stress. Therefore, in this work, polymeric membranes formed by organic–inorganic hybrid materials called ureasil-polyether for modified glucose release were developed in order to overcome the problems posed by a of lack of this nutrient. Thus, membranes formed by polymeric blend of polypropylene oxide (PPO4000) and polyethylene oxide (PEO500) with 6% glucose incorporation were developed. Physical–chemical characterization techniques were performed, as well as tests that evaluated thermal properties, bioactivity, swelling, and release in SBF solution. The results of the swelling test showed an increase in membrane mass correlated with an increase in the concentration of ureasil-PEO500 in the polymeric blends. The membranes showed adequate resistance when subjected to the application of a high compression force (15 N). X-ray diffraction (XRD) evidenced peaks corresponding to orthorhombic crystalline organization, but the absence of glucose-related peaks showed characteristics of the amorphous regions of hybrid materials, likely due to solubilization. Thermogravimetry (TG) and differential scanning calorimetry (DSC) analyses showed that the thermal events attributed to glucose and hybrid materials were similar to that seen in the literature, however when glucose was incorporated into the PEO500, an increase in rigidity occurs. In PPO400, and in the blends of both materials, there was a slight decrease in Tg values. The smaller contact angle for the ureasil-PEO500 membrane revealed the more hydrophilic character of the material compared to other membranes. The membranes showed bioactivity and hemocompatibility in vitro. The in vitro release test revealed that it is possible to control the release rate of glucose and the kinetic analysis revealed a release mechanism characteristic of anomalous transport kinetics. Thus, we can conclude that ureasil-polyether membranes have great potential to be used as a glucose release system, and their future application has the potential to optimize the bone regeneration process.School of Pharmaceutical Sciences São Paulo State University (UNESP), SPGraduate Program in Pharmaceutical Sciences Biological and Health Sciences Center State University of Paraiba (UEPB), PBSchool of Pharmaceutical Sciences São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)State University of Paraiba (UEPB)da Silva, Camila Garcia [UNESP]Monteiro, João Rodrigues [UNESP]Oshiro-Júnior, João AugustoChiavacci, Leila Aparecida [UNESP]2023-07-29T16:14:57Z2023-07-29T16:14:57Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pharmaceutics15051474Pharmaceutics, v. 15, n. 5, 2023.1999-4923http://hdl.handle.net/11449/24999710.3390/pharmaceutics150514742-s2.0-85160408677Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceuticsinfo:eu-repo/semantics/openAccess2024-06-24T13:45:18Zoai:repositorio.unesp.br:11449/249997Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-24T13:45:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration |
title |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration |
spellingShingle |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration da Silva, Camila Garcia [UNESP] bone regeneration glucose release organic–inorganic hybrids polyethylene oxide polypropylene oxide sol–gel process |
title_short |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration |
title_full |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration |
title_fullStr |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration |
title_full_unstemmed |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration |
title_sort |
Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration |
author |
da Silva, Camila Garcia [UNESP] |
author_facet |
da Silva, Camila Garcia [UNESP] Monteiro, João Rodrigues [UNESP] Oshiro-Júnior, João Augusto Chiavacci, Leila Aparecida [UNESP] |
author_role |
author |
author2 |
Monteiro, João Rodrigues [UNESP] Oshiro-Júnior, João Augusto Chiavacci, Leila Aparecida [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) State University of Paraiba (UEPB) |
dc.contributor.author.fl_str_mv |
da Silva, Camila Garcia [UNESP] Monteiro, João Rodrigues [UNESP] Oshiro-Júnior, João Augusto Chiavacci, Leila Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
bone regeneration glucose release organic–inorganic hybrids polyethylene oxide polypropylene oxide sol–gel process |
topic |
bone regeneration glucose release organic–inorganic hybrids polyethylene oxide polypropylene oxide sol–gel process |
description |
The application of mesenchymal stem cells (MSC) in bone tissue regeneration can have unpredictable results due to the low survival of cells in the process since the lack of oxygen and nutrients promotes metabolic stress. Therefore, in this work, polymeric membranes formed by organic–inorganic hybrid materials called ureasil-polyether for modified glucose release were developed in order to overcome the problems posed by a of lack of this nutrient. Thus, membranes formed by polymeric blend of polypropylene oxide (PPO4000) and polyethylene oxide (PEO500) with 6% glucose incorporation were developed. Physical–chemical characterization techniques were performed, as well as tests that evaluated thermal properties, bioactivity, swelling, and release in SBF solution. The results of the swelling test showed an increase in membrane mass correlated with an increase in the concentration of ureasil-PEO500 in the polymeric blends. The membranes showed adequate resistance when subjected to the application of a high compression force (15 N). X-ray diffraction (XRD) evidenced peaks corresponding to orthorhombic crystalline organization, but the absence of glucose-related peaks showed characteristics of the amorphous regions of hybrid materials, likely due to solubilization. Thermogravimetry (TG) and differential scanning calorimetry (DSC) analyses showed that the thermal events attributed to glucose and hybrid materials were similar to that seen in the literature, however when glucose was incorporated into the PEO500, an increase in rigidity occurs. In PPO400, and in the blends of both materials, there was a slight decrease in Tg values. The smaller contact angle for the ureasil-PEO500 membrane revealed the more hydrophilic character of the material compared to other membranes. The membranes showed bioactivity and hemocompatibility in vitro. The in vitro release test revealed that it is possible to control the release rate of glucose and the kinetic analysis revealed a release mechanism characteristic of anomalous transport kinetics. Thus, we can conclude that ureasil-polyether membranes have great potential to be used as a glucose release system, and their future application has the potential to optimize the bone regeneration process. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:14:57Z 2023-07-29T16:14:57Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pharmaceutics15051474 Pharmaceutics, v. 15, n. 5, 2023. 1999-4923 http://hdl.handle.net/11449/249997 10.3390/pharmaceutics15051474 2-s2.0-85160408677 |
url |
http://dx.doi.org/10.3390/pharmaceutics15051474 http://hdl.handle.net/11449/249997 |
identifier_str_mv |
Pharmaceutics, v. 15, n. 5, 2023. 1999-4923 10.3390/pharmaceutics15051474 2-s2.0-85160408677 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceutics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045256539668480 |