Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0967199417000715 http://hdl.handle.net/11449/163801 |
Resumo: | This study investigated and analysed survival, growth and macro-and microscopic damage during the development of zebrafish embryos up to the adult stage after undergoing cooling. The embryos at 50% epiboly stage were selected, submerged in cryoprotectant solution of methanol and sucrose, cooled gradually to 0 +/- 2 degrees C temperature, and divided into two groups with different storage times (6 and 18 h). Subsequently, the embryos were reheated, rehydrated and incubated normally. The experiment lasted 5 months and, from hatching onward, the larvae were examined, collected and processed at pre-established time intervals. The hatching rate was significantly higher for the larvae stored for 18 h compared with the 6-h group. However, embryos from this group gave rise to a larger number of malformations, and these were much more severe compared with those in the 6 h group, which led to a higher mortality in the long term. Regarding larval length, the animals of the 6 h group had higher mean total length compared with the 18 h group, but both treatments were inferior to the control. Numerous macro-andmicroscopic malformations were observed and, in both treatments, only the morphologically normal individuals were able to develop to the adult stage, with organ development similar to the control, except for the gonads that were still undifferentiated in treated animals. |
id |
UNSP_497cb5869e108241336c50c681c139f8 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/163801 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio)Fish embryosLow temperatureMalformationMicroscopyMorphologyThis study investigated and analysed survival, growth and macro-and microscopic damage during the development of zebrafish embryos up to the adult stage after undergoing cooling. The embryos at 50% epiboly stage were selected, submerged in cryoprotectant solution of methanol and sucrose, cooled gradually to 0 +/- 2 degrees C temperature, and divided into two groups with different storage times (6 and 18 h). Subsequently, the embryos were reheated, rehydrated and incubated normally. The experiment lasted 5 months and, from hatching onward, the larvae were examined, collected and processed at pre-established time intervals. The hatching rate was significantly higher for the larvae stored for 18 h compared with the 6-h group. However, embryos from this group gave rise to a larger number of malformations, and these were much more severe compared with those in the 6 h group, which led to a higher mortality in the long term. Regarding larval length, the animals of the 6 h group had higher mean total length compared with the 18 h group, but both treatments were inferior to the control. Numerous macro-andmicroscopic malformations were observed and, in both treatments, only the morphologically normal individuals were able to develop to the adult stage, with organ development similar to the control, except for the gonads that were still undifferentiated in treated animals.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ CAUNESP, Aquaculture Ctr, Jaboticabal, SP, BrazilSao Paulo State Univ, Dept Anim Morphol & Physiol, Campus Jaboticabal, Sao Paulo, BrazilAccess Way Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ CAUNESP, Aquaculture Ctr, Jaboticabal, SP, BrazilSao Paulo State Univ, Dept Anim Morphol & Physiol, Campus Jaboticabal, Sao Paulo, BrazilFAPESP: 2012/24909-5Cambridge Univ PressUniversidade Estadual Paulista (Unesp)Faria Paes, Maria do Carmo [UNESP]Okada Nakaghi, Laura Satiko [UNESP]2018-11-26T17:45:02Z2018-11-26T17:45:02Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article76-88application/pdfhttp://dx.doi.org/10.1017/S0967199417000715Zygote. New York: Cambridge Univ Press, v. 26, n. 1, p. 76-88, 2018.0967-1994http://hdl.handle.net/11449/16380110.1017/S0967199417000715WOS:000424128200008WOS000424128200008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygote0,387info:eu-repo/semantics/openAccess2024-06-06T18:42:13Zoai:repositorio.unesp.br:11449/163801Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:31:43.611998Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) |
title |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) |
spellingShingle |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) Faria Paes, Maria do Carmo [UNESP] Fish embryos Low temperature Malformation Microscopy Morphology |
title_short |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) |
title_full |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) |
title_fullStr |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) |
title_full_unstemmed |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) |
title_sort |
Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio) |
author |
Faria Paes, Maria do Carmo [UNESP] |
author_facet |
Faria Paes, Maria do Carmo [UNESP] Okada Nakaghi, Laura Satiko [UNESP] |
author_role |
author |
author2 |
Okada Nakaghi, Laura Satiko [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Faria Paes, Maria do Carmo [UNESP] Okada Nakaghi, Laura Satiko [UNESP] |
dc.subject.por.fl_str_mv |
Fish embryos Low temperature Malformation Microscopy Morphology |
topic |
Fish embryos Low temperature Malformation Microscopy Morphology |
description |
This study investigated and analysed survival, growth and macro-and microscopic damage during the development of zebrafish embryos up to the adult stage after undergoing cooling. The embryos at 50% epiboly stage were selected, submerged in cryoprotectant solution of methanol and sucrose, cooled gradually to 0 +/- 2 degrees C temperature, and divided into two groups with different storage times (6 and 18 h). Subsequently, the embryos were reheated, rehydrated and incubated normally. The experiment lasted 5 months and, from hatching onward, the larvae were examined, collected and processed at pre-established time intervals. The hatching rate was significantly higher for the larvae stored for 18 h compared with the 6-h group. However, embryos from this group gave rise to a larger number of malformations, and these were much more severe compared with those in the 6 h group, which led to a higher mortality in the long term. Regarding larval length, the animals of the 6 h group had higher mean total length compared with the 18 h group, but both treatments were inferior to the control. Numerous macro-andmicroscopic malformations were observed and, in both treatments, only the morphologically normal individuals were able to develop to the adult stage, with organ development similar to the control, except for the gonads that were still undifferentiated in treated animals. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:45:02Z 2018-11-26T17:45:02Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199417000715 Zygote. New York: Cambridge Univ Press, v. 26, n. 1, p. 76-88, 2018. 0967-1994 http://hdl.handle.net/11449/163801 10.1017/S0967199417000715 WOS:000424128200008 WOS000424128200008.pdf |
url |
http://dx.doi.org/10.1017/S0967199417000715 http://hdl.handle.net/11449/163801 |
identifier_str_mv |
Zygote. New York: Cambridge Univ Press, v. 26, n. 1, p. 76-88, 2018. 0967-1994 10.1017/S0967199417000715 WOS:000424128200008 WOS000424128200008.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zygote 0,387 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
76-88 application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129331153600512 |