A modular autoinduction device for control of gene expression in Bacillus subtilis

Detalhes bibliográficos
Autor(a) principal: Corrêa, Graciely Gomes [UNESP]
Data de Publicação: 2020
Outros Autores: Lins, Milca Rachel da Costa Ribeiro [UNESP], Silva, Bruna Fernandes [UNESP], de Paiva, Gabriela Barbosa [UNESP], Zocca, Vitoria Fernanda Bertolazzi [UNESP], Ribeiro, Nathan Vinicius [UNESP], Picheli, Flavio Pereira [UNESP], Mack, Matthias, Pedrolli, Danielle Biscaro [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ymben.2020.03.012
http://hdl.handle.net/11449/198900
Resumo: Intense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly applicable tools are available to achieve this. We present an autonomous control of gene expression mediated by quorum sensing in Bacillus subtilis, able to self-monitor and induce expression without human supervision. Two variations of the induction module and seven of the response module were engineered generating a range of induction folds and strengths for gene expression control. Our strongest response promoter is 2.5 and 3.2 times stronger than the well-characterized promoters PsrfA and Pveg, respectively. We applied our strongest autoinduction device for the production of the vitamin B2. This study presents a toolbox of autoinduction modules for B. subtilis that is modular and tunable.
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spelling A modular autoinduction device for control of gene expression in Bacillus subtilisAutoinductionBacillus subtilisDynamic regulationLuxR/IQuorum sensingSynthetic biologyIntense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly applicable tools are available to achieve this. We present an autonomous control of gene expression mediated by quorum sensing in Bacillus subtilis, able to self-monitor and induce expression without human supervision. Two variations of the induction module and seven of the response module were engineered generating a range of induction folds and strengths for gene expression control. Our strongest response promoter is 2.5 and 3.2 times stronger than the well-characterized promoters PsrfA and Pveg, respectively. We applied our strongest autoinduction device for the production of the vitamin B2. This study presents a toolbox of autoinduction modules for B. subtilis that is modular and tunable.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1Mannheim University of Applied Sciences Institute for Technical Microbiology, Paul-Wittsack-Str. 10Universidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1CAPES: 001FAPESP: 2014/17564–7CAPES: CAPES SWE 290109/2017–5Universidade Estadual Paulista (Unesp)Institute for Technical MicrobiologyCorrêa, Graciely Gomes [UNESP]Lins, Milca Rachel da Costa Ribeiro [UNESP]Silva, Bruna Fernandes [UNESP]de Paiva, Gabriela Barbosa [UNESP]Zocca, Vitoria Fernanda Bertolazzi [UNESP]Ribeiro, Nathan Vinicius [UNESP]Picheli, Flavio Pereira [UNESP]Mack, MatthiasPedrolli, Danielle Biscaro [UNESP]2020-12-12T01:25:01Z2020-12-12T01:25:01Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article326-334http://dx.doi.org/10.1016/j.ymben.2020.03.012Metabolic Engineering, v. 61, p. 326-334.1096-71841096-7176http://hdl.handle.net/11449/19890010.1016/j.ymben.2020.03.0122-s2.0-85085553634Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMetabolic Engineeringinfo:eu-repo/semantics/openAccess2021-10-22T20:56:19Zoai:repositorio.unesp.br:11449/198900Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:35:30.891161Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A modular autoinduction device for control of gene expression in Bacillus subtilis
title A modular autoinduction device for control of gene expression in Bacillus subtilis
spellingShingle A modular autoinduction device for control of gene expression in Bacillus subtilis
Corrêa, Graciely Gomes [UNESP]
Autoinduction
Bacillus subtilis
Dynamic regulation
LuxR/I
Quorum sensing
Synthetic biology
title_short A modular autoinduction device for control of gene expression in Bacillus subtilis
title_full A modular autoinduction device for control of gene expression in Bacillus subtilis
title_fullStr A modular autoinduction device for control of gene expression in Bacillus subtilis
title_full_unstemmed A modular autoinduction device for control of gene expression in Bacillus subtilis
title_sort A modular autoinduction device for control of gene expression in Bacillus subtilis
author Corrêa, Graciely Gomes [UNESP]
author_facet Corrêa, Graciely Gomes [UNESP]
Lins, Milca Rachel da Costa Ribeiro [UNESP]
Silva, Bruna Fernandes [UNESP]
de Paiva, Gabriela Barbosa [UNESP]
Zocca, Vitoria Fernanda Bertolazzi [UNESP]
Ribeiro, Nathan Vinicius [UNESP]
Picheli, Flavio Pereira [UNESP]
Mack, Matthias
Pedrolli, Danielle Biscaro [UNESP]
author_role author
author2 Lins, Milca Rachel da Costa Ribeiro [UNESP]
Silva, Bruna Fernandes [UNESP]
de Paiva, Gabriela Barbosa [UNESP]
Zocca, Vitoria Fernanda Bertolazzi [UNESP]
Ribeiro, Nathan Vinicius [UNESP]
Picheli, Flavio Pereira [UNESP]
Mack, Matthias
Pedrolli, Danielle Biscaro [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Institute for Technical Microbiology
dc.contributor.author.fl_str_mv Corrêa, Graciely Gomes [UNESP]
Lins, Milca Rachel da Costa Ribeiro [UNESP]
Silva, Bruna Fernandes [UNESP]
de Paiva, Gabriela Barbosa [UNESP]
Zocca, Vitoria Fernanda Bertolazzi [UNESP]
Ribeiro, Nathan Vinicius [UNESP]
Picheli, Flavio Pereira [UNESP]
Mack, Matthias
Pedrolli, Danielle Biscaro [UNESP]
dc.subject.por.fl_str_mv Autoinduction
Bacillus subtilis
Dynamic regulation
LuxR/I
Quorum sensing
Synthetic biology
topic Autoinduction
Bacillus subtilis
Dynamic regulation
LuxR/I
Quorum sensing
Synthetic biology
description Intense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly applicable tools are available to achieve this. We present an autonomous control of gene expression mediated by quorum sensing in Bacillus subtilis, able to self-monitor and induce expression without human supervision. Two variations of the induction module and seven of the response module were engineered generating a range of induction folds and strengths for gene expression control. Our strongest response promoter is 2.5 and 3.2 times stronger than the well-characterized promoters PsrfA and Pveg, respectively. We applied our strongest autoinduction device for the production of the vitamin B2. This study presents a toolbox of autoinduction modules for B. subtilis that is modular and tunable.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:25:01Z
2020-12-12T01:25:01Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ymben.2020.03.012
Metabolic Engineering, v. 61, p. 326-334.
1096-7184
1096-7176
http://hdl.handle.net/11449/198900
10.1016/j.ymben.2020.03.012
2-s2.0-85085553634
url http://dx.doi.org/10.1016/j.ymben.2020.03.012
http://hdl.handle.net/11449/198900
identifier_str_mv Metabolic Engineering, v. 61, p. 326-334.
1096-7184
1096-7176
10.1016/j.ymben.2020.03.012
2-s2.0-85085553634
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Metabolic Engineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 326-334
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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