Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4137/EBO.S15364 http://hdl.handle.net/11449/172278 |
Resumo: | Fetal hemoglobin (Hb F) is an important genetic modulator of the beta-hemoglobinopathies. The regulation of Hb F levels is influenced by transcription factors. We used phylogenetic footprinting to screen transcription factors that have binding sites in HBG1 and HBG2 genes’ noncoding regions in order to know the genetic determinants of the Hb F expression. Our analysis showed 354 conserved motifs in the noncoding regions of HBG1 gene and 231 motifs in the HBG2 gene between the analyzed species. Of these motifs, 13 showed relation to Hb F regulation: cell division cycle-5 (CDC5), myelo-blastosis viral oncogene homolog (c-MYB), transcription factor CP2 (TFCP2), GATA binding protein 1 (GATA-1), GATA binding protein 2 (GATA-2), nuclear factor erythroid 2 (NF-E2), nuclear transcription factor Y (NF-Y), runt-related transcription factor 1 (RUNX-1), T-cell acute lymphocytic leukemia 1 (TAL-1), YY1 transcription factor (YY1), beta protein 1 (BP1), chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), and paired box 1 (PAX-1). The last three motifs were conserved only in the noncoding regions of the HBG1 gene. The understanding of genetic elements involved in the maintenance of high Hb F levels may provide new efficient therapeutic strategies in the beta-hemoglobinopathies treatment, promoting reduction in clinical complications of these genetic disorders. |
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Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprintingBeta-hemoglobinopathiesHb FPhylogenetic footprintingTranscription factorsFetal hemoglobin (Hb F) is an important genetic modulator of the beta-hemoglobinopathies. The regulation of Hb F levels is influenced by transcription factors. We used phylogenetic footprinting to screen transcription factors that have binding sites in HBG1 and HBG2 genes’ noncoding regions in order to know the genetic determinants of the Hb F expression. Our analysis showed 354 conserved motifs in the noncoding regions of HBG1 gene and 231 motifs in the HBG2 gene between the analyzed species. Of these motifs, 13 showed relation to Hb F regulation: cell division cycle-5 (CDC5), myelo-blastosis viral oncogene homolog (c-MYB), transcription factor CP2 (TFCP2), GATA binding protein 1 (GATA-1), GATA binding protein 2 (GATA-2), nuclear factor erythroid 2 (NF-E2), nuclear transcription factor Y (NF-Y), runt-related transcription factor 1 (RUNX-1), T-cell acute lymphocytic leukemia 1 (TAL-1), YY1 transcription factor (YY1), beta protein 1 (BP1), chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), and paired box 1 (PAX-1). The last three motifs were conserved only in the noncoding regions of the HBG1 gene. The understanding of genetic elements involved in the maintenance of high Hb F levels may provide new efficient therapeutic strategies in the beta-hemoglobinopathies treatment, promoting reduction in clinical complications of these genetic disorders.Laboratory of Hemoglobin and Genetics of Hematologic Diseases Department of Biology São Paulo State University – UNESP/IBILCELaboratory of Hemoglobin and Genetics of Hematologic Diseases Department of Biology São Paulo State University – UNESP/IBILCEUniversidade Estadual Paulista (Unesp)Carrocini, Gisele Cristine de Souza [UNESP]Venancio, Larissa Paola Rodrigues [UNESP]Bonini-Domingos, Claudia Regina [UNESP]2018-12-11T16:59:30Z2018-12-11T16:59:30Z2015-10-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article239-244application/pdfhttp://dx.doi.org/10.4137/EBO.S15364Evolutionary Bioinformatics, v. 11, p. 239-244.1176-9343http://hdl.handle.net/11449/17227810.4137/EBO.S153642-s2.0-849494988612-s2.0-8494949886132794280661767190000-0002-4603-9467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvolutionary Bioinformatics0,900info:eu-repo/semantics/openAccess2024-01-09T06:27:38Zoai:repositorio.unesp.br:11449/172278Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:32:25.489535Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting |
title |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting |
spellingShingle |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting Carrocini, Gisele Cristine de Souza [UNESP] Beta-hemoglobinopathies Hb F Phylogenetic footprinting Transcription factors |
title_short |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting |
title_full |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting |
title_fullStr |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting |
title_full_unstemmed |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting |
title_sort |
Screening of transcription factors involved in fetal hemoglobin regulation using phylogenetic footprinting |
author |
Carrocini, Gisele Cristine de Souza [UNESP] |
author_facet |
Carrocini, Gisele Cristine de Souza [UNESP] Venancio, Larissa Paola Rodrigues [UNESP] Bonini-Domingos, Claudia Regina [UNESP] |
author_role |
author |
author2 |
Venancio, Larissa Paola Rodrigues [UNESP] Bonini-Domingos, Claudia Regina [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Carrocini, Gisele Cristine de Souza [UNESP] Venancio, Larissa Paola Rodrigues [UNESP] Bonini-Domingos, Claudia Regina [UNESP] |
dc.subject.por.fl_str_mv |
Beta-hemoglobinopathies Hb F Phylogenetic footprinting Transcription factors |
topic |
Beta-hemoglobinopathies Hb F Phylogenetic footprinting Transcription factors |
description |
Fetal hemoglobin (Hb F) is an important genetic modulator of the beta-hemoglobinopathies. The regulation of Hb F levels is influenced by transcription factors. We used phylogenetic footprinting to screen transcription factors that have binding sites in HBG1 and HBG2 genes’ noncoding regions in order to know the genetic determinants of the Hb F expression. Our analysis showed 354 conserved motifs in the noncoding regions of HBG1 gene and 231 motifs in the HBG2 gene between the analyzed species. Of these motifs, 13 showed relation to Hb F regulation: cell division cycle-5 (CDC5), myelo-blastosis viral oncogene homolog (c-MYB), transcription factor CP2 (TFCP2), GATA binding protein 1 (GATA-1), GATA binding protein 2 (GATA-2), nuclear factor erythroid 2 (NF-E2), nuclear transcription factor Y (NF-Y), runt-related transcription factor 1 (RUNX-1), T-cell acute lymphocytic leukemia 1 (TAL-1), YY1 transcription factor (YY1), beta protein 1 (BP1), chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), and paired box 1 (PAX-1). The last three motifs were conserved only in the noncoding regions of the HBG1 gene. The understanding of genetic elements involved in the maintenance of high Hb F levels may provide new efficient therapeutic strategies in the beta-hemoglobinopathies treatment, promoting reduction in clinical complications of these genetic disorders. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-27 2018-12-11T16:59:30Z 2018-12-11T16:59:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4137/EBO.S15364 Evolutionary Bioinformatics, v. 11, p. 239-244. 1176-9343 http://hdl.handle.net/11449/172278 10.4137/EBO.S15364 2-s2.0-84949498861 2-s2.0-84949498861 3279428066176719 0000-0002-4603-9467 |
url |
http://dx.doi.org/10.4137/EBO.S15364 http://hdl.handle.net/11449/172278 |
identifier_str_mv |
Evolutionary Bioinformatics, v. 11, p. 239-244. 1176-9343 10.4137/EBO.S15364 2-s2.0-84949498861 3279428066176719 0000-0002-4603-9467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evolutionary Bioinformatics 0,900 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
239-244 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129434772832256 |