Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil

Detalhes bibliográficos
Autor(a) principal: Úngari, Letícia Pereira [UNESP]
Data de Publicação: 2018
Outros Autores: Santos, André Luiz Quagliatto, O’Dwyer, Lucia Helena [UNESP], da Silva, Maria Regina Lucas [UNESP], Rodrigues Santos, Thais Carneiro, da Cunha, Maria Júlia Rodrigues, de Melo Costa Pinto, Rogério, Cury, Márcia Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00436-018-6092-3
http://hdl.handle.net/11449/188117
Resumo: Species of Hepatozoon are parasites frequently recorded in snakes. The species identification of this genus was based mostly on the gametocyte morphology and morphometric calculations. For more reliable results, molecular characterization, an initial step for the correct identification of Hepatozoon species, has been used. This study aimed to determine the prevalence and identification of Hepatozoon species in captive snakes from Brazil. To that end, morphological, morphometric, and molecular data were obtained. A total of 157 snakes; 128 venomous (Crotalus durissus) and 29 non-venomous (Epicrates crassus and Boa constrictor) were screened for Hepatozoon blood parasites. Using light microscopy, 20 (12.78%) snakes were found positive for Hepatozoon spp., of which 6/29 (20.7%) were non-venomous and 14/128 (10.9%) were venomous; all with low parasitemia. Polymerase chain reaction (PCR), performed with the primers HepF300/Hep900, confirmed all 20 (100%) samples positive for hemogregarines. Species of Hepatozoon were identified from eight sequenced samples. Two previously described species, Hepatozoon cuestensis and Hepatozoon musa, were identified. The present study is the first to report H. musa within the snake hosts E. crassus and C. durrisus. In addition, a potentially new Hepatozoon species from B. constrictor was identified.
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spelling Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from BrazilHepatozoon sppNon-venomousPCRSnakesVenomousSpecies of Hepatozoon are parasites frequently recorded in snakes. The species identification of this genus was based mostly on the gametocyte morphology and morphometric calculations. For more reliable results, molecular characterization, an initial step for the correct identification of Hepatozoon species, has been used. This study aimed to determine the prevalence and identification of Hepatozoon species in captive snakes from Brazil. To that end, morphological, morphometric, and molecular data were obtained. A total of 157 snakes; 128 venomous (Crotalus durissus) and 29 non-venomous (Epicrates crassus and Boa constrictor) were screened for Hepatozoon blood parasites. Using light microscopy, 20 (12.78%) snakes were found positive for Hepatozoon spp., of which 6/29 (20.7%) were non-venomous and 14/128 (10.9%) were venomous; all with low parasitemia. Polymerase chain reaction (PCR), performed with the primers HepF300/Hep900, confirmed all 20 (100%) samples positive for hemogregarines. Species of Hepatozoon were identified from eight sequenced samples. Two previously described species, Hepatozoon cuestensis and Hepatozoon musa, were identified. The present study is the first to report H. musa within the snake hosts E. crassus and C. durrisus. In addition, a potentially new Hepatozoon species from B. constrictor was identified.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Laboratório de Sorologia e Biologia Molecular de Parasitos Instituto de Ciências Biomédicas-ICBIM Universidade Federal de Uberlândia-UFUInstituto de Biociências Campus de Botucatu Departamento de Parasitologia UNESP-Univ. Estadual Paulista, Distrito de Rubião JuniorLaboratório de Ensino e Pesquisa em Animais Silvestres-LAPAS Faculdade de Medicina Veterinária Universidade Federal de Uberlândia-UFUFaculdade de Matemática-FAMAT Centro de Ciências e Tecnologia Universidade Federal de Uberlândia—UFUInstituto de Biociências Campus de Botucatu Departamento de Parasitologia UNESP-Univ. Estadual Paulista, Distrito de Rubião JuniorUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Úngari, Letícia Pereira [UNESP]Santos, André Luiz QuagliattoO’Dwyer, Lucia Helena [UNESP]da Silva, Maria Regina Lucas [UNESP]Rodrigues Santos, Thais Carneiroda Cunha, Maria Júlia Rodriguesde Melo Costa Pinto, RogérioCury, Márcia Cristina2019-10-06T15:57:46Z2019-10-06T15:57:46Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3857-3865http://dx.doi.org/10.1007/s00436-018-6092-3Parasitology Research, v. 117, n. 12, p. 3857-3865, 2018.1432-19550932-0113http://hdl.handle.net/11449/18811710.1007/s00436-018-6092-32-s2.0-85054051902Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Researchinfo:eu-repo/semantics/openAccess2021-10-22T18:13:14Zoai:repositorio.unesp.br:11449/188117Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:47:35.276888Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
title Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
spellingShingle Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
Úngari, Letícia Pereira [UNESP]
Hepatozoon spp
Non-venomous
PCR
Snakes
Venomous
title_short Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
title_full Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
title_fullStr Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
title_full_unstemmed Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
title_sort Molecular characterization and identification of Hepatozoon species Miller, 1908 (Apicomplexa: Adeleina: Hepatozoidae) in captive snakes from Brazil
author Úngari, Letícia Pereira [UNESP]
author_facet Úngari, Letícia Pereira [UNESP]
Santos, André Luiz Quagliatto
O’Dwyer, Lucia Helena [UNESP]
da Silva, Maria Regina Lucas [UNESP]
Rodrigues Santos, Thais Carneiro
da Cunha, Maria Júlia Rodrigues
de Melo Costa Pinto, Rogério
Cury, Márcia Cristina
author_role author
author2 Santos, André Luiz Quagliatto
O’Dwyer, Lucia Helena [UNESP]
da Silva, Maria Regina Lucas [UNESP]
Rodrigues Santos, Thais Carneiro
da Cunha, Maria Júlia Rodrigues
de Melo Costa Pinto, Rogério
Cury, Márcia Cristina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Úngari, Letícia Pereira [UNESP]
Santos, André Luiz Quagliatto
O’Dwyer, Lucia Helena [UNESP]
da Silva, Maria Regina Lucas [UNESP]
Rodrigues Santos, Thais Carneiro
da Cunha, Maria Júlia Rodrigues
de Melo Costa Pinto, Rogério
Cury, Márcia Cristina
dc.subject.por.fl_str_mv Hepatozoon spp
Non-venomous
PCR
Snakes
Venomous
topic Hepatozoon spp
Non-venomous
PCR
Snakes
Venomous
description Species of Hepatozoon are parasites frequently recorded in snakes. The species identification of this genus was based mostly on the gametocyte morphology and morphometric calculations. For more reliable results, molecular characterization, an initial step for the correct identification of Hepatozoon species, has been used. This study aimed to determine the prevalence and identification of Hepatozoon species in captive snakes from Brazil. To that end, morphological, morphometric, and molecular data were obtained. A total of 157 snakes; 128 venomous (Crotalus durissus) and 29 non-venomous (Epicrates crassus and Boa constrictor) were screened for Hepatozoon blood parasites. Using light microscopy, 20 (12.78%) snakes were found positive for Hepatozoon spp., of which 6/29 (20.7%) were non-venomous and 14/128 (10.9%) were venomous; all with low parasitemia. Polymerase chain reaction (PCR), performed with the primers HepF300/Hep900, confirmed all 20 (100%) samples positive for hemogregarines. Species of Hepatozoon were identified from eight sequenced samples. Two previously described species, Hepatozoon cuestensis and Hepatozoon musa, were identified. The present study is the first to report H. musa within the snake hosts E. crassus and C. durrisus. In addition, a potentially new Hepatozoon species from B. constrictor was identified.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
2019-10-06T15:57:46Z
2019-10-06T15:57:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00436-018-6092-3
Parasitology Research, v. 117, n. 12, p. 3857-3865, 2018.
1432-1955
0932-0113
http://hdl.handle.net/11449/188117
10.1007/s00436-018-6092-3
2-s2.0-85054051902
url http://dx.doi.org/10.1007/s00436-018-6092-3
http://hdl.handle.net/11449/188117
identifier_str_mv Parasitology Research, v. 117, n. 12, p. 3857-3865, 2018.
1432-1955
0932-0113
10.1007/s00436-018-6092-3
2-s2.0-85054051902
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasitology Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3857-3865
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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