Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2016.01518 http://hdl.handle.net/11449/178433 |
Resumo: | Termites are considered one of the most efficient decomposers of lignocelluloses on Earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (CAZymes). Recently, a set of Pro-oxidant, Antioxidant, and Detoxification enzymes (PAD) were also correlated with the metabolism of carbohydrates and lignin in termites. The lower termite Coptotermes gestroi is considered the main urban pest in Brazil, causing damage to wood constructions. Recently, analysis of the enzymatic repertoire of C. gestroi unveiled the presence of different CAZymes. Because the gene profile of CAZy/PAD enzymes endogenously synthesized by C. gestroi and also by their symbiotic protists remains unclear, the aim of this study was to explore the eukaryotic repertoire of these enzymes in worker and soldier castes of C. gestroi. Our findings showed that worker and soldier castes present similar repertoires of CAZy/PAD enzymes, and also confirmed that endo-glucanases (GH9) and beta-glucosidases (GH1) were the most important glycoside hydrolase families related to lignocellulose degradation in both castes. Classical cellulases such as exo-glucanases (GH7) and endo-glucanases (GH5 and GH45), as well as classical xylanases (GH10 and GH11), were found in both castes only taxonomically related to protists, highlighting the importance of symbiosis in C. gestroi. Moreover, our analysis revealed the presence of Auxiliary Activity enzyme families (AAs), which could be related to lignin modifications in termite digestomes. In conclusion, this report expanded the knowledge on genes and proteins related to CAZy/PAD enzymes from worker and soldier castes of lower termites, revealing new potential enzyme candidates for second-generation biofuel processes. |
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Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroiAuxiliary activity enzymesCarbohydrate-active enzymesCAZySecond-generation biofuelsTermite digestomestermitesTermites are considered one of the most efficient decomposers of lignocelluloses on Earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (CAZymes). Recently, a set of Pro-oxidant, Antioxidant, and Detoxification enzymes (PAD) were also correlated with the metabolism of carbohydrates and lignin in termites. The lower termite Coptotermes gestroi is considered the main urban pest in Brazil, causing damage to wood constructions. Recently, analysis of the enzymatic repertoire of C. gestroi unveiled the presence of different CAZymes. Because the gene profile of CAZy/PAD enzymes endogenously synthesized by C. gestroi and also by their symbiotic protists remains unclear, the aim of this study was to explore the eukaryotic repertoire of these enzymes in worker and soldier castes of C. gestroi. Our findings showed that worker and soldier castes present similar repertoires of CAZy/PAD enzymes, and also confirmed that endo-glucanases (GH9) and beta-glucosidases (GH1) were the most important glycoside hydrolase families related to lignocellulose degradation in both castes. Classical cellulases such as exo-glucanases (GH7) and endo-glucanases (GH5 and GH45), as well as classical xylanases (GH10 and GH11), were found in both castes only taxonomically related to protists, highlighting the importance of symbiosis in C. gestroi. Moreover, our analysis revealed the presence of Auxiliary Activity enzyme families (AAs), which could be related to lignin modifications in termite digestomes. In conclusion, this report expanded the knowledge on genes and proteins related to CAZy/PAD enzymes from worker and soldier castes of lower termites, revealing new potential enzyme candidates for second-generation biofuel processes.Laborat�rio Nacional de Ci�ncia e Tecnologia do Bioetanol (CTBE) Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)Departamento de Bioqu�mica e Biologia Tecidual Universidade Estadual de Campinas (UNICAMP)Laborat�rio de Gen�mica e Express�o Universidade Estadual de Campinas (UNICAMP)Centro de Hematologia e Hemoterapia (Hemocentro) Universidade Estadual de Campinas (UNICAMP)Laborat�rio de Espectrometria de Massas Laborat�rio Nacional de Bioci�ncias (LNBIO) Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)Departamento de Biologia Instituto de Bioci�ncias Universidade Estadual Paulista (UNESP)Departamento de Biologia Instituto de Bioci�ncias Universidade Estadual Paulista (UNESP)Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Franco Cairo, Jo�o P.L.Carazzolle, Marcelo F.Leonardo, Fl�via C.Mofatto, Luciana S.Brenelli, L�via B.Gon�alves, Thiago A.Uchima, Cristiane A.Domingues, Rom�nia R.Alvarez, Thabata M.Tramontina, RobsonVidal, Ramon O.Costa, Fernando F.Costa-Leonardo, Ana M. [UNESP]Paes Leme, Adriana F.Pereira, Gon�alo A.G.Squina, Fabio M.2018-12-11T17:30:16Z2018-12-11T17:30:16Z2016-10-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3389/fmicb.2016.01518Frontiers in Microbiology, v. 7, n. OCT, 2016.1664-302Xhttp://hdl.handle.net/11449/17843310.3389/fmicb.2016.015182-s2.0-849970519262-s2.0-84997051926.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2023-12-05T06:20:00Zoai:repositorio.unesp.br:11449/178433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:34:07.697926Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi |
title |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi |
spellingShingle |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi Franco Cairo, Jo�o P.L. Auxiliary activity enzymes Carbohydrate-active enzymes CAZy Second-generation biofuels Termite digestomes termites |
title_short |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi |
title_full |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi |
title_fullStr |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi |
title_full_unstemmed |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi |
title_sort |
Expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite Coptotermes gestroi |
author |
Franco Cairo, Jo�o P.L. |
author_facet |
Franco Cairo, Jo�o P.L. Carazzolle, Marcelo F. Leonardo, Fl�via C. Mofatto, Luciana S. Brenelli, L�via B. Gon�alves, Thiago A. Uchima, Cristiane A. Domingues, Rom�nia R. Alvarez, Thabata M. Tramontina, Robson Vidal, Ramon O. Costa, Fernando F. Costa-Leonardo, Ana M. [UNESP] Paes Leme, Adriana F. Pereira, Gon�alo A.G. Squina, Fabio M. |
author_role |
author |
author2 |
Carazzolle, Marcelo F. Leonardo, Fl�via C. Mofatto, Luciana S. Brenelli, L�via B. Gon�alves, Thiago A. Uchima, Cristiane A. Domingues, Rom�nia R. Alvarez, Thabata M. Tramontina, Robson Vidal, Ramon O. Costa, Fernando F. Costa-Leonardo, Ana M. [UNESP] Paes Leme, Adriana F. Pereira, Gon�alo A.G. Squina, Fabio M. |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro Nacional de Pesquisa em Energia e Materiais (CNPEM) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Franco Cairo, Jo�o P.L. Carazzolle, Marcelo F. Leonardo, Fl�via C. Mofatto, Luciana S. Brenelli, L�via B. Gon�alves, Thiago A. Uchima, Cristiane A. Domingues, Rom�nia R. Alvarez, Thabata M. Tramontina, Robson Vidal, Ramon O. Costa, Fernando F. Costa-Leonardo, Ana M. [UNESP] Paes Leme, Adriana F. Pereira, Gon�alo A.G. Squina, Fabio M. |
dc.subject.por.fl_str_mv |
Auxiliary activity enzymes Carbohydrate-active enzymes CAZy Second-generation biofuels Termite digestomes termites |
topic |
Auxiliary activity enzymes Carbohydrate-active enzymes CAZy Second-generation biofuels Termite digestomes termites |
description |
Termites are considered one of the most efficient decomposers of lignocelluloses on Earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (CAZymes). Recently, a set of Pro-oxidant, Antioxidant, and Detoxification enzymes (PAD) were also correlated with the metabolism of carbohydrates and lignin in termites. The lower termite Coptotermes gestroi is considered the main urban pest in Brazil, causing damage to wood constructions. Recently, analysis of the enzymatic repertoire of C. gestroi unveiled the presence of different CAZymes. Because the gene profile of CAZy/PAD enzymes endogenously synthesized by C. gestroi and also by their symbiotic protists remains unclear, the aim of this study was to explore the eukaryotic repertoire of these enzymes in worker and soldier castes of C. gestroi. Our findings showed that worker and soldier castes present similar repertoires of CAZy/PAD enzymes, and also confirmed that endo-glucanases (GH9) and beta-glucosidases (GH1) were the most important glycoside hydrolase families related to lignocellulose degradation in both castes. Classical cellulases such as exo-glucanases (GH7) and endo-glucanases (GH5 and GH45), as well as classical xylanases (GH10 and GH11), were found in both castes only taxonomically related to protists, highlighting the importance of symbiosis in C. gestroi. Moreover, our analysis revealed the presence of Auxiliary Activity enzyme families (AAs), which could be related to lignin modifications in termite digestomes. In conclusion, this report expanded the knowledge on genes and proteins related to CAZy/PAD enzymes from worker and soldier castes of lower termites, revealing new potential enzyme candidates for second-generation biofuel processes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-13 2018-12-11T17:30:16Z 2018-12-11T17:30:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2016.01518 Frontiers in Microbiology, v. 7, n. OCT, 2016. 1664-302X http://hdl.handle.net/11449/178433 10.3389/fmicb.2016.01518 2-s2.0-84997051926 2-s2.0-84997051926.pdf |
url |
http://dx.doi.org/10.3389/fmicb.2016.01518 http://hdl.handle.net/11449/178433 |
identifier_str_mv |
Frontiers in Microbiology, v. 7, n. OCT, 2016. 1664-302X 10.3389/fmicb.2016.01518 2-s2.0-84997051926 2-s2.0-84997051926.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129087220219904 |