Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1601-5223.2000.00147.x http://hdl.handle.net/11449/66347 |
Resumo: | The mitotic and meiotic chromosomes of the beetles Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) were analysed using standard staining, C-banding and silver impregnation techniques. We determine the diploid and haploid chromosome numbers, the sex determination system and describe the chromosomal morphology, the C-banding pattern and the chromosome(s) bearing NORs (nucleolar organizer regions). Both species shown 2n = 20 chromosomes, the chromosomal meioformula 9 + Xyp, and regular chromosome segregation during anaphases I and II. The chromosomes of E. atomaria are basically metacentric or submetacentric and P. dermestoides chromosomes are submetacentric or subtelocentric. In both beetles the constitutive heterochromatin is located in the pericentromeric region in all autosomes and in the Xp chromosome; additional C-bands were observed in telomeric region of the short arm in some autosomes in P. dermestoides. The yp chromosome did not show typical C-bands in these species. As for the synaptonemal complex, the nucleolar material is associated to the 7th bivalent in E. atomaria and 3rd and 7th bivalents in P. dermestoides. Strong silver impregnated material was observed in association with Xyp in light and electron microscopy preparations in these species and this material was interpreted to be related to nucleolar material. |
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Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniquesChromosomeColeopteraHeterochromatinKaryotypeMeiosisNucleolusPachytenePolyphag aanaphaseanimal cellautosomebeetlecentromerechromosomechromosome analysischromosome banding patternchromosome C bandchromosome NORchromosome numberchromosome segregationchromosome structurechromosome Xpchromosome Ypdiploidyhaploidyheterochromatinkaryotypemalemeiosismitosismorphologynonhumanpachytenesex determinationsilver impregnationstainingsynaptonemal complextechniqueAnimalsBeetlesCell NucleusChromosome BandingFemaleKaryotypingMaleSex Determination (Genetics)Synaptonemal ComplexX ChromosomeAnimaliaEpicautaEpicauta atomariaInsectaMeloidaePalembusPalembus dermestoidesTenebrionidaeThe mitotic and meiotic chromosomes of the beetles Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) were analysed using standard staining, C-banding and silver impregnation techniques. We determine the diploid and haploid chromosome numbers, the sex determination system and describe the chromosomal morphology, the C-banding pattern and the chromosome(s) bearing NORs (nucleolar organizer regions). Both species shown 2n = 20 chromosomes, the chromosomal meioformula 9 + Xyp, and regular chromosome segregation during anaphases I and II. The chromosomes of E. atomaria are basically metacentric or submetacentric and P. dermestoides chromosomes are submetacentric or subtelocentric. In both beetles the constitutive heterochromatin is located in the pericentromeric region in all autosomes and in the Xp chromosome; additional C-bands were observed in telomeric region of the short arm in some autosomes in P. dermestoides. The yp chromosome did not show typical C-bands in these species. As for the synaptonemal complex, the nucleolar material is associated to the 7th bivalent in E. atomaria and 3rd and 7th bivalents in P. dermestoides. Strong silver impregnated material was observed in association with Xyp in light and electron microscopy preparations in these species and this material was interpreted to be related to nucleolar material.Departamento de Biologia Geral Setor de Ciências Biológicas e da Saúde UEPGDepartamento de Biologia Geral CCBS Universidade Federal de Viçosa UFV Viçosa, MGDepartamento de Biologia Institute de Biociências UNESP, Rio Claro, SPDepartamento de Biologia Geral Universidade Estadual de Ponta Grossa Setor de Ciências Biológicas e du Saúde, Av. Carlos Cavalcanti, n.4748, Uvarunus, Ponta Grossu, ParunaDepartamento de Biologia Institute de Biociências UNESP, Rio Claro, SPUniversidade Estadual de Ponta Grossa (UEPG)Universidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Almeida, Mara Cristina deZacaro, Adilson ArizaCella, Doralice Maria [UNESP]2014-05-27T11:19:59Z2014-05-27T11:19:59Z2000-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-157application/pdfhttp://dx.doi.org/10.1111/j.1601-5223.2000.00147.xHereditas, v. 133, n. 2, p. 147-157, 2000.0018-0661http://hdl.handle.net/11449/6634710.1111/j.1601-5223.2000.00147.xWOS:0001681218000072-s2.0-00344543292-s2.0-0034454329.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHereditas0,278info:eu-repo/semantics/openAccess2023-12-04T06:16:05Zoai:repositorio.unesp.br:11449/66347Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:29:17.030609Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques |
title |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques |
spellingShingle |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques Almeida, Mara Cristina de Chromosome Coleoptera Heterochromatin Karyotype Meiosis Nucleolus Pachytene Polyphag a anaphase animal cell autosome beetle centromere chromosome chromosome analysis chromosome banding pattern chromosome C band chromosome NOR chromosome number chromosome segregation chromosome structure chromosome Xp chromosome Yp diploidy haploidy heterochromatin karyotype male meiosis mitosis morphology nonhuman pachytene sex determination silver impregnation staining synaptonemal complex technique Animals Beetles Cell Nucleus Chromosome Banding Female Karyotyping Male Sex Determination (Genetics) Synaptonemal Complex X Chromosome Animalia Epicauta Epicauta atomaria Insecta Meloidae Palembus Palembus dermestoides Tenebrionidae |
title_short |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques |
title_full |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques |
title_fullStr |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques |
title_full_unstemmed |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques |
title_sort |
Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques |
author |
Almeida, Mara Cristina de |
author_facet |
Almeida, Mara Cristina de Zacaro, Adilson Ariza Cella, Doralice Maria [UNESP] |
author_role |
author |
author2 |
Zacaro, Adilson Ariza Cella, Doralice Maria [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Ponta Grossa (UEPG) Universidade Federal de Viçosa (UFV) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Almeida, Mara Cristina de Zacaro, Adilson Ariza Cella, Doralice Maria [UNESP] |
dc.subject.por.fl_str_mv |
Chromosome Coleoptera Heterochromatin Karyotype Meiosis Nucleolus Pachytene Polyphag a anaphase animal cell autosome beetle centromere chromosome chromosome analysis chromosome banding pattern chromosome C band chromosome NOR chromosome number chromosome segregation chromosome structure chromosome Xp chromosome Yp diploidy haploidy heterochromatin karyotype male meiosis mitosis morphology nonhuman pachytene sex determination silver impregnation staining synaptonemal complex technique Animals Beetles Cell Nucleus Chromosome Banding Female Karyotyping Male Sex Determination (Genetics) Synaptonemal Complex X Chromosome Animalia Epicauta Epicauta atomaria Insecta Meloidae Palembus Palembus dermestoides Tenebrionidae |
topic |
Chromosome Coleoptera Heterochromatin Karyotype Meiosis Nucleolus Pachytene Polyphag a anaphase animal cell autosome beetle centromere chromosome chromosome analysis chromosome banding pattern chromosome C band chromosome NOR chromosome number chromosome segregation chromosome structure chromosome Xp chromosome Yp diploidy haploidy heterochromatin karyotype male meiosis mitosis morphology nonhuman pachytene sex determination silver impregnation staining synaptonemal complex technique Animals Beetles Cell Nucleus Chromosome Banding Female Karyotyping Male Sex Determination (Genetics) Synaptonemal Complex X Chromosome Animalia Epicauta Epicauta atomaria Insecta Meloidae Palembus Palembus dermestoides Tenebrionidae |
description |
The mitotic and meiotic chromosomes of the beetles Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) were analysed using standard staining, C-banding and silver impregnation techniques. We determine the diploid and haploid chromosome numbers, the sex determination system and describe the chromosomal morphology, the C-banding pattern and the chromosome(s) bearing NORs (nucleolar organizer regions). Both species shown 2n = 20 chromosomes, the chromosomal meioformula 9 + Xyp, and regular chromosome segregation during anaphases I and II. The chromosomes of E. atomaria are basically metacentric or submetacentric and P. dermestoides chromosomes are submetacentric or subtelocentric. In both beetles the constitutive heterochromatin is located in the pericentromeric region in all autosomes and in the Xp chromosome; additional C-bands were observed in telomeric region of the short arm in some autosomes in P. dermestoides. The yp chromosome did not show typical C-bands in these species. As for the synaptonemal complex, the nucleolar material is associated to the 7th bivalent in E. atomaria and 3rd and 7th bivalents in P. dermestoides. Strong silver impregnated material was observed in association with Xyp in light and electron microscopy preparations in these species and this material was interpreted to be related to nucleolar material. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-12-01 2014-05-27T11:19:59Z 2014-05-27T11:19:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1601-5223.2000.00147.x Hereditas, v. 133, n. 2, p. 147-157, 2000. 0018-0661 http://hdl.handle.net/11449/66347 10.1111/j.1601-5223.2000.00147.x WOS:000168121800007 2-s2.0-0034454329 2-s2.0-0034454329.pdf |
url |
http://dx.doi.org/10.1111/j.1601-5223.2000.00147.x http://hdl.handle.net/11449/66347 |
identifier_str_mv |
Hereditas, v. 133, n. 2, p. 147-157, 2000. 0018-0661 10.1111/j.1601-5223.2000.00147.x WOS:000168121800007 2-s2.0-0034454329 2-s2.0-0034454329.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hereditas 0,278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
147-157 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129076582416384 |