Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.clim.2021.108917 http://hdl.handle.net/11449/230169 |
Resumo: | This study evaluated the in vitro modulatory effect of vitamin D (VD) on T cells, by determining the expression of STATs and the transcription factors of each CD4+ T cell subsets. Twenty women with preeclampsia (PE) and 20 normotensive pregnant women were studied. Peripheral blood mononuclear cells were cultured with or without VD to analyse the STATs and transcription factors by flow cytometry, and cytokines production by ELISA. The plasma levels of VD were lower in the PE group. Treatment of cells with VD decreased STAT1/STAT4/T-bet, STAT3/RORγt, and increased STAT6/GATA-3 and STAT5/FoxP3 in preeclamptic women. Treatment with VD also decreased the levels of inflammatory cytokines and increased IL-10 and TGF-β. This hormone exerts immunomodulatory effects on the STAT signalling pathway, shifting the inflammatory profiles, Th1/Th17 cells to Th2/Treg profiles, and it can be suggested as a promising strategy to regulate the systemic inflammatory response in PE. |
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Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsiaActivators of transcriptionPreeclampsiaT cell subsetsTranscription factorsVitamin DThis study evaluated the in vitro modulatory effect of vitamin D (VD) on T cells, by determining the expression of STATs and the transcription factors of each CD4+ T cell subsets. Twenty women with preeclampsia (PE) and 20 normotensive pregnant women were studied. Peripheral blood mononuclear cells were cultured with or without VD to analyse the STATs and transcription factors by flow cytometry, and cytokines production by ELISA. The plasma levels of VD were lower in the PE group. Treatment of cells with VD decreased STAT1/STAT4/T-bet, STAT3/RORγt, and increased STAT6/GATA-3 and STAT5/FoxP3 in preeclamptic women. Treatment with VD also decreased the levels of inflammatory cytokines and increased IL-10 and TGF-β. This hormone exerts immunomodulatory effects on the STAT signalling pathway, shifting the inflammatory profiles, Th1/Th17 cells to Th2/Treg profiles, and it can be suggested as a promising strategy to regulate the systemic inflammatory response in PE.ASCRS Research FoundationDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESPDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESPDepartment of Health Science Oeste Paulista University-UNOESTEDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESPDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESPUniversidade Estadual Paulista (UNESP)Oeste Paulista University-UNOESTERibeiro, Vanessa Rocha [UNESP]Romao-Veiga, Mariana [UNESP]Nunes, Priscila Rezeck [UNESP]de Oliveira, Larissa Ragozo Cardoso [UNESP]Romagnoli, Graziela GorettiPeracoli, Jose Carlos [UNESP]Peracoli, Maria Terezinha Serrao [UNESP]2022-04-29T08:38:14Z2022-04-29T08:38:14Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.clim.2021.108917Clinical Immunology, v. 234.1521-70351521-6616http://hdl.handle.net/11449/23016910.1016/j.clim.2021.1089172-s2.0-85122297032Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Immunologyinfo:eu-repo/semantics/openAccess2024-08-16T14:12:31Zoai:repositorio.unesp.br:11449/230169Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:12:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia |
title |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia |
spellingShingle |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia Ribeiro, Vanessa Rocha [UNESP] Activators of transcription Preeclampsia T cell subsets Transcription factors Vitamin D |
title_short |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia |
title_full |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia |
title_fullStr |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia |
title_full_unstemmed |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia |
title_sort |
Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia |
author |
Ribeiro, Vanessa Rocha [UNESP] |
author_facet |
Ribeiro, Vanessa Rocha [UNESP] Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] de Oliveira, Larissa Ragozo Cardoso [UNESP] Romagnoli, Graziela Goretti Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author_role |
author |
author2 |
Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] de Oliveira, Larissa Ragozo Cardoso [UNESP] Romagnoli, Graziela Goretti Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Oeste Paulista University-UNOESTE |
dc.contributor.author.fl_str_mv |
Ribeiro, Vanessa Rocha [UNESP] Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] de Oliveira, Larissa Ragozo Cardoso [UNESP] Romagnoli, Graziela Goretti Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
dc.subject.por.fl_str_mv |
Activators of transcription Preeclampsia T cell subsets Transcription factors Vitamin D |
topic |
Activators of transcription Preeclampsia T cell subsets Transcription factors Vitamin D |
description |
This study evaluated the in vitro modulatory effect of vitamin D (VD) on T cells, by determining the expression of STATs and the transcription factors of each CD4+ T cell subsets. Twenty women with preeclampsia (PE) and 20 normotensive pregnant women were studied. Peripheral blood mononuclear cells were cultured with or without VD to analyse the STATs and transcription factors by flow cytometry, and cytokines production by ELISA. The plasma levels of VD were lower in the PE group. Treatment of cells with VD decreased STAT1/STAT4/T-bet, STAT3/RORγt, and increased STAT6/GATA-3 and STAT5/FoxP3 in preeclamptic women. Treatment with VD also decreased the levels of inflammatory cytokines and increased IL-10 and TGF-β. This hormone exerts immunomodulatory effects on the STAT signalling pathway, shifting the inflammatory profiles, Th1/Th17 cells to Th2/Treg profiles, and it can be suggested as a promising strategy to regulate the systemic inflammatory response in PE. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:38:14Z 2022-04-29T08:38:14Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.clim.2021.108917 Clinical Immunology, v. 234. 1521-7035 1521-6616 http://hdl.handle.net/11449/230169 10.1016/j.clim.2021.108917 2-s2.0-85122297032 |
url |
http://dx.doi.org/10.1016/j.clim.2021.108917 http://hdl.handle.net/11449/230169 |
identifier_str_mv |
Clinical Immunology, v. 234. 1521-7035 1521-6616 10.1016/j.clim.2021.108917 2-s2.0-85122297032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128176102047744 |