Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Vanessa Rocha [UNESP]
Data de Publicação: 2022
Outros Autores: Romao-Veiga, Mariana [UNESP], Nunes, Priscila Rezeck [UNESP], de Oliveira, Larissa Ragozo Cardoso [UNESP], Romagnoli, Graziela Goretti, Peracoli, Jose Carlos [UNESP], Peracoli, Maria Terezinha Serrao [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.clim.2021.108917
http://hdl.handle.net/11449/230169
Resumo: This study evaluated the in vitro modulatory effect of vitamin D (VD) on T cells, by determining the expression of STATs and the transcription factors of each CD4+ T cell subsets. Twenty women with preeclampsia (PE) and 20 normotensive pregnant women were studied. Peripheral blood mononuclear cells were cultured with or without VD to analyse the STATs and transcription factors by flow cytometry, and cytokines production by ELISA. The plasma levels of VD were lower in the PE group. Treatment of cells with VD decreased STAT1/STAT4/T-bet, STAT3/RORγt, and increased STAT6/GATA-3 and STAT5/FoxP3 in preeclamptic women. Treatment with VD also decreased the levels of inflammatory cytokines and increased IL-10 and TGF-β. This hormone exerts immunomodulatory effects on the STAT signalling pathway, shifting the inflammatory profiles, Th1/Th17 cells to Th2/Treg profiles, and it can be suggested as a promising strategy to regulate the systemic inflammatory response in PE.
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spelling Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsiaActivators of transcriptionPreeclampsiaT cell subsetsTranscription factorsVitamin DThis study evaluated the in vitro modulatory effect of vitamin D (VD) on T cells, by determining the expression of STATs and the transcription factors of each CD4+ T cell subsets. Twenty women with preeclampsia (PE) and 20 normotensive pregnant women were studied. Peripheral blood mononuclear cells were cultured with or without VD to analyse the STATs and transcription factors by flow cytometry, and cytokines production by ELISA. The plasma levels of VD were lower in the PE group. Treatment of cells with VD decreased STAT1/STAT4/T-bet, STAT3/RORγt, and increased STAT6/GATA-3 and STAT5/FoxP3 in preeclamptic women. Treatment with VD also decreased the levels of inflammatory cytokines and increased IL-10 and TGF-β. This hormone exerts immunomodulatory effects on the STAT signalling pathway, shifting the inflammatory profiles, Th1/Th17 cells to Th2/Treg profiles, and it can be suggested as a promising strategy to regulate the systemic inflammatory response in PE.ASCRS Research FoundationDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESPDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESPDepartment of Health Science Oeste Paulista University-UNOESTEDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESPDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESPUniversidade Estadual Paulista (UNESP)Oeste Paulista University-UNOESTERibeiro, Vanessa Rocha [UNESP]Romao-Veiga, Mariana [UNESP]Nunes, Priscila Rezeck [UNESP]de Oliveira, Larissa Ragozo Cardoso [UNESP]Romagnoli, Graziela GorettiPeracoli, Jose Carlos [UNESP]Peracoli, Maria Terezinha Serrao [UNESP]2022-04-29T08:38:14Z2022-04-29T08:38:14Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.clim.2021.108917Clinical Immunology, v. 234.1521-70351521-6616http://hdl.handle.net/11449/23016910.1016/j.clim.2021.1089172-s2.0-85122297032Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Immunologyinfo:eu-repo/semantics/openAccess2024-08-16T14:12:31Zoai:repositorio.unesp.br:11449/230169Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:12:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
title Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
spellingShingle Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
Ribeiro, Vanessa Rocha [UNESP]
Activators of transcription
Preeclampsia
T cell subsets
Transcription factors
Vitamin D
title_short Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
title_full Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
title_fullStr Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
title_full_unstemmed Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
title_sort Immunomodulatory effect of vitamin D on the STATs and transcription factors of CD4+ T cell subsets in pregnant women with preeclampsia
author Ribeiro, Vanessa Rocha [UNESP]
author_facet Ribeiro, Vanessa Rocha [UNESP]
Romao-Veiga, Mariana [UNESP]
Nunes, Priscila Rezeck [UNESP]
de Oliveira, Larissa Ragozo Cardoso [UNESP]
Romagnoli, Graziela Goretti
Peracoli, Jose Carlos [UNESP]
Peracoli, Maria Terezinha Serrao [UNESP]
author_role author
author2 Romao-Veiga, Mariana [UNESP]
Nunes, Priscila Rezeck [UNESP]
de Oliveira, Larissa Ragozo Cardoso [UNESP]
Romagnoli, Graziela Goretti
Peracoli, Jose Carlos [UNESP]
Peracoli, Maria Terezinha Serrao [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Oeste Paulista University-UNOESTE
dc.contributor.author.fl_str_mv Ribeiro, Vanessa Rocha [UNESP]
Romao-Veiga, Mariana [UNESP]
Nunes, Priscila Rezeck [UNESP]
de Oliveira, Larissa Ragozo Cardoso [UNESP]
Romagnoli, Graziela Goretti
Peracoli, Jose Carlos [UNESP]
Peracoli, Maria Terezinha Serrao [UNESP]
dc.subject.por.fl_str_mv Activators of transcription
Preeclampsia
T cell subsets
Transcription factors
Vitamin D
topic Activators of transcription
Preeclampsia
T cell subsets
Transcription factors
Vitamin D
description This study evaluated the in vitro modulatory effect of vitamin D (VD) on T cells, by determining the expression of STATs and the transcription factors of each CD4+ T cell subsets. Twenty women with preeclampsia (PE) and 20 normotensive pregnant women were studied. Peripheral blood mononuclear cells were cultured with or without VD to analyse the STATs and transcription factors by flow cytometry, and cytokines production by ELISA. The plasma levels of VD were lower in the PE group. Treatment of cells with VD decreased STAT1/STAT4/T-bet, STAT3/RORγt, and increased STAT6/GATA-3 and STAT5/FoxP3 in preeclamptic women. Treatment with VD also decreased the levels of inflammatory cytokines and increased IL-10 and TGF-β. This hormone exerts immunomodulatory effects on the STAT signalling pathway, shifting the inflammatory profiles, Th1/Th17 cells to Th2/Treg profiles, and it can be suggested as a promising strategy to regulate the systemic inflammatory response in PE.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:38:14Z
2022-04-29T08:38:14Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.clim.2021.108917
Clinical Immunology, v. 234.
1521-7035
1521-6616
http://hdl.handle.net/11449/230169
10.1016/j.clim.2021.108917
2-s2.0-85122297032
url http://dx.doi.org/10.1016/j.clim.2021.108917
http://hdl.handle.net/11449/230169
identifier_str_mv Clinical Immunology, v. 234.
1521-7035
1521-6616
10.1016/j.clim.2021.108917
2-s2.0-85122297032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Immunology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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