Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces

Detalhes bibliográficos
Autor(a) principal: Carvalho, Maria Das Graças
Data de Publicação: 1991
Outros Autores: Guimarães, Semíramis [UNESP], Silva, Edward Felix
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0036-46651991000500007
http://hdl.handle.net/11449/211123
Resumo: Serum samples were obtained from 154 individuals infected with Entamoeba histolytica (78 symptomatic and 76 asymptomatic). Twelve had trophozoites in the feces whereas 142 had only cysts. The sera were used to test the existence of antibodies anti-Entamoeba histolytica employing the Indirect Hemagglutination (IHA), Indirect Immunofluoresccnce (IFAT), Complement Fixation Reaction (CFR) and Counterimmunoelectrophoresis (CIEP). For those individuals with trophozoites in their feces, 75.0 were positive by IHA and IFAT, 83.0 by CFR and 41.7 by CIEP. In individuals who had only cysts, positive results by the same tests were respectively, 5.6%, 12.0%, 19.0% and 5.6%. The difference in relation to the tilers of antibodies detected through IHA, IFAT, CFR and CIEP and in relation to the presence of trophozoites or cysts in the feces was significative for four immunological reactions when X², was employed (P < 0.05).
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spelling Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the fecesAnticorpos anti-Entamoeba histolylica em indivíduos com amebíase intestinal apresentando cistos e/ou trofozoitas nas fezesEntamoeba histolyticaIHAIFATCFRCIEPcyststrophozoitesSerum samples were obtained from 154 individuals infected with Entamoeba histolytica (78 symptomatic and 76 asymptomatic). Twelve had trophozoites in the feces whereas 142 had only cysts. The sera were used to test the existence of antibodies anti-Entamoeba histolytica employing the Indirect Hemagglutination (IHA), Indirect Immunofluoresccnce (IFAT), Complement Fixation Reaction (CFR) and Counterimmunoelectrophoresis (CIEP). For those individuals with trophozoites in their feces, 75.0 were positive by IHA and IFAT, 83.0 by CFR and 41.7 by CIEP. In individuals who had only cysts, positive results by the same tests were respectively, 5.6%, 12.0%, 19.0% and 5.6%. The difference in relation to the tilers of antibodies detected through IHA, IFAT, CFR and CIEP and in relation to the presence of trophozoites or cysts in the feces was significative for four immunological reactions when X², was employed (P < 0.05).Amostras de soros foram obtidas de 154 indivíduos comprovadamente parasitados pela Entamoeba histolytica (78 sintomáticos c 76 assintomáticos). Doze apresentavam trofozoitas nas fezes, enquanto 142 tinham apenas cistos. Os soros foram utilizados para testar a ocorrência de anticorpos anti-Entamoeba histolytica, empregando-se para tal, Reação de Hemaglutinação Indireta (HAI), Reação de Imunofluorescência Indireta (RIFI), Reação de Fixação de Complemento (RFC) e Contraimunoeletroforese (CIEP). Entre os soros dos indivíduos com trofozoitas em suas fezes, 75,0% foram positivos para HAI e RIFI, 83,3% por RFC, e 41,7% por CIEP. Nos indivíduos que tinham apenas cistos, resultados positivos pelos mesmos testes foram respectivamente, 5,6%; 12,0%; 19,0% c 5,6%. A diferença cm relação aos títulos de anticorpos detectados através de HAI, RIFI, RFC e CIEP e em relação à presença de trofozoitas ou cistos nas fezes foi significativa para as quatro reações imunológicas, quando X² foi empregado (P < 0,05).Universidade Federal de Minas Gerais, Pharmacy FacultyUniversidade Estadual Paulista, Bioscience InstituteUniversidade Federal de Minas Gerais, Institute of Biological ScienceUniversidade Estadual Paulista, Bioscience InstituteInstituto de Medicina TropicalUniversidade Federal de Minas GeraisUniversidade Estadual Paulista (Unesp)Carvalho, Maria Das GraçasGuimarães, Semíramis [UNESP]Silva, Edward Felix2021-07-14T10:19:37Z2021-07-14T10:19:37Z1991-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article379-383application/pdfhttp://dx.doi.org/10.1590/S0036-46651991000500007Revista do Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil: Instituto de Medicina Tropical, v. 33, n. 5, p. 379-383, 1991.1678-9946http://hdl.handle.net/11449/21112310.1590/S0036-46651991000500007S0036-46651991000500007S0036-46651991000500007.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccess2023-11-02T06:11:37Zoai:repositorio.unesp.br:11449/211123Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:44:40.324645Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
Anticorpos anti-Entamoeba histolylica em indivíduos com amebíase intestinal apresentando cistos e/ou trofozoitas nas fezes
title Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
spellingShingle Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
Carvalho, Maria Das Graças
Entamoeba histolytica
IHA
IFAT
CFR
CIEP
cysts
trophozoites
title_short Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
title_full Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
title_fullStr Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
title_full_unstemmed Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
title_sort Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces
author Carvalho, Maria Das Graças
author_facet Carvalho, Maria Das Graças
Guimarães, Semíramis [UNESP]
Silva, Edward Felix
author_role author
author2 Guimarães, Semíramis [UNESP]
Silva, Edward Felix
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Federal de Minas Gerais
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Carvalho, Maria Das Graças
Guimarães, Semíramis [UNESP]
Silva, Edward Felix
dc.subject.por.fl_str_mv Entamoeba histolytica
IHA
IFAT
CFR
CIEP
cysts
trophozoites
topic Entamoeba histolytica
IHA
IFAT
CFR
CIEP
cysts
trophozoites
description Serum samples were obtained from 154 individuals infected with Entamoeba histolytica (78 symptomatic and 76 asymptomatic). Twelve had trophozoites in the feces whereas 142 had only cysts. The sera were used to test the existence of antibodies anti-Entamoeba histolytica employing the Indirect Hemagglutination (IHA), Indirect Immunofluoresccnce (IFAT), Complement Fixation Reaction (CFR) and Counterimmunoelectrophoresis (CIEP). For those individuals with trophozoites in their feces, 75.0 were positive by IHA and IFAT, 83.0 by CFR and 41.7 by CIEP. In individuals who had only cysts, positive results by the same tests were respectively, 5.6%, 12.0%, 19.0% and 5.6%. The difference in relation to the tilers of antibodies detected through IHA, IFAT, CFR and CIEP and in relation to the presence of trophozoites or cysts in the feces was significative for four immunological reactions when X², was employed (P < 0.05).
publishDate 1991
dc.date.none.fl_str_mv 1991-10
2021-07-14T10:19:37Z
2021-07-14T10:19:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0036-46651991000500007
Revista do Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil: Instituto de Medicina Tropical, v. 33, n. 5, p. 379-383, 1991.
1678-9946
http://hdl.handle.net/11449/211123
10.1590/S0036-46651991000500007
S0036-46651991000500007
S0036-46651991000500007.pdf
url http://dx.doi.org/10.1590/S0036-46651991000500007
http://hdl.handle.net/11449/211123
identifier_str_mv Revista do Instituto de Medicina Tropical de São Paulo. São Paulo, SP, Brazil: Instituto de Medicina Tropical, v. 33, n. 5, p. 379-383, 1991.
1678-9946
10.1590/S0036-46651991000500007
S0036-46651991000500007
S0036-46651991000500007.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 379-383
application/pdf
dc.publisher.none.fl_str_mv Instituto de Medicina Tropical
publisher.none.fl_str_mv Instituto de Medicina Tropical
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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