Minimum blood lactate and muscle protein of rats during swimming exercise
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/225143 |
Resumo: | Few studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7±0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 mm) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[14C] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4±0.01) and equivalent (6.4±0.006) to MBL but increased, progressively, with the high load. There were no differences in protein synthesis (pmol/mg.h) among rest values (65.2±3.4) and after-exercise supporting the loads below (61.5±1.3) and the equivalent (60.7±1.7) to MBL but there was a decrease with the high load (36.6±2.0). Protein breakdown rates (pmol/g.h) increase after exercise supporting the loads below (227.0±6.1), equivalent (227.9±6.0) and high (363.6±7.1) to MBL in relation to the rest (214.3±6.0). The results indicate the viability of the application of LMT in studies with rats since it detected alterations imposed by exercise. |
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Minimum blood lactate and muscle protein of rats during swimming exerciseLactate minimum testMinimum blood lactateProtein degradationProtein synthesisRatsFew studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7±0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 mm) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[14C] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4±0.01) and equivalent (6.4±0.006) to MBL but increased, progressively, with the high load. There were no differences in protein synthesis (pmol/mg.h) among rest values (65.2±3.4) and after-exercise supporting the loads below (61.5±1.3) and the equivalent (60.7±1.7) to MBL but there was a decrease with the high load (36.6±2.0). Protein breakdown rates (pmol/g.h) increase after exercise supporting the loads below (227.0±6.1), equivalent (227.9±6.0) and high (363.6±7.1) to MBL in relation to the rest (214.3±6.0). The results indicate the viability of the application of LMT in studies with rats since it detected alterations imposed by exercise.UNESP Sao Paulo State UniversityDept. of Physical Education University of Sao Paulo State (UNESP) Campus of Rio Claro, 24-A Avenue, number 1515, Bela Vista, 13506-900, Rio Claro-State, São PauloUNESP Sao Paulo State UniversityDept. of Physical Education University of Sao Paulo State (UNESP) Campus of Rio Claro, 24-A Avenue, number 1515, Bela Vista, 13506-900, Rio Claro-State, São PauloUniversidade Estadual Paulista (UNESP)Voltarelli, F. A. [UNESP]Gobatto, C. A. [UNESP]De Mello, M. A.R. [UNESP]2022-04-28T20:40:12Z2022-04-28T20:40:12Z2008-04-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article23-34Biology of Sport, v. 25, n. 1, p. 23-34, 2008.0860-021Xhttp://hdl.handle.net/11449/2251432-s2.0-42549168074Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiology of Sportinfo:eu-repo/semantics/openAccess2022-04-28T20:40:12Zoai:repositorio.unesp.br:11449/225143Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:47:26.933449Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Minimum blood lactate and muscle protein of rats during swimming exercise |
title |
Minimum blood lactate and muscle protein of rats during swimming exercise |
spellingShingle |
Minimum blood lactate and muscle protein of rats during swimming exercise Voltarelli, F. A. [UNESP] Lactate minimum test Minimum blood lactate Protein degradation Protein synthesis Rats |
title_short |
Minimum blood lactate and muscle protein of rats during swimming exercise |
title_full |
Minimum blood lactate and muscle protein of rats during swimming exercise |
title_fullStr |
Minimum blood lactate and muscle protein of rats during swimming exercise |
title_full_unstemmed |
Minimum blood lactate and muscle protein of rats during swimming exercise |
title_sort |
Minimum blood lactate and muscle protein of rats during swimming exercise |
author |
Voltarelli, F. A. [UNESP] |
author_facet |
Voltarelli, F. A. [UNESP] Gobatto, C. A. [UNESP] De Mello, M. A.R. [UNESP] |
author_role |
author |
author2 |
Gobatto, C. A. [UNESP] De Mello, M. A.R. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Voltarelli, F. A. [UNESP] Gobatto, C. A. [UNESP] De Mello, M. A.R. [UNESP] |
dc.subject.por.fl_str_mv |
Lactate minimum test Minimum blood lactate Protein degradation Protein synthesis Rats |
topic |
Lactate minimum test Minimum blood lactate Protein degradation Protein synthesis Rats |
description |
Few studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7±0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 mm) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[14C] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4±0.01) and equivalent (6.4±0.006) to MBL but increased, progressively, with the high load. There were no differences in protein synthesis (pmol/mg.h) among rest values (65.2±3.4) and after-exercise supporting the loads below (61.5±1.3) and the equivalent (60.7±1.7) to MBL but there was a decrease with the high load (36.6±2.0). Protein breakdown rates (pmol/g.h) increase after exercise supporting the loads below (227.0±6.1), equivalent (227.9±6.0) and high (363.6±7.1) to MBL in relation to the rest (214.3±6.0). The results indicate the viability of the application of LMT in studies with rats since it detected alterations imposed by exercise. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-04-29 2022-04-28T20:40:12Z 2022-04-28T20:40:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Biology of Sport, v. 25, n. 1, p. 23-34, 2008. 0860-021X http://hdl.handle.net/11449/225143 2-s2.0-42549168074 |
identifier_str_mv |
Biology of Sport, v. 25, n. 1, p. 23-34, 2008. 0860-021X 2-s2.0-42549168074 |
url |
http://hdl.handle.net/11449/225143 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biology of Sport |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
23-34 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129552490168320 |