Minimum blood lactate and muscle protein of rats during swimming exercise

Detalhes bibliográficos
Autor(a) principal: Voltarelli, F. A. [UNESP]
Data de Publicação: 2008
Outros Autores: Gobatto, C. A. [UNESP], De Mello, M. A.R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/225143
Resumo: Few studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7±0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 mm) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[14C] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4±0.01) and equivalent (6.4±0.006) to MBL but increased, progressively, with the high load. There were no differences in protein synthesis (pmol/mg.h) among rest values (65.2±3.4) and after-exercise supporting the loads below (61.5±1.3) and the equivalent (60.7±1.7) to MBL but there was a decrease with the high load (36.6±2.0). Protein breakdown rates (pmol/g.h) increase after exercise supporting the loads below (227.0±6.1), equivalent (227.9±6.0) and high (363.6±7.1) to MBL in relation to the rest (214.3±6.0). The results indicate the viability of the application of LMT in studies with rats since it detected alterations imposed by exercise.
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spelling Minimum blood lactate and muscle protein of rats during swimming exerciseLactate minimum testMinimum blood lactateProtein degradationProtein synthesisRatsFew studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7±0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 mm) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[14C] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4±0.01) and equivalent (6.4±0.006) to MBL but increased, progressively, with the high load. There were no differences in protein synthesis (pmol/mg.h) among rest values (65.2±3.4) and after-exercise supporting the loads below (61.5±1.3) and the equivalent (60.7±1.7) to MBL but there was a decrease with the high load (36.6±2.0). Protein breakdown rates (pmol/g.h) increase after exercise supporting the loads below (227.0±6.1), equivalent (227.9±6.0) and high (363.6±7.1) to MBL in relation to the rest (214.3±6.0). The results indicate the viability of the application of LMT in studies with rats since it detected alterations imposed by exercise.UNESP Sao Paulo State UniversityDept. of Physical Education University of Sao Paulo State (UNESP) Campus of Rio Claro, 24-A Avenue, number 1515, Bela Vista, 13506-900, Rio Claro-State, São PauloUNESP Sao Paulo State UniversityDept. of Physical Education University of Sao Paulo State (UNESP) Campus of Rio Claro, 24-A Avenue, number 1515, Bela Vista, 13506-900, Rio Claro-State, São PauloUniversidade Estadual Paulista (UNESP)Voltarelli, F. A. [UNESP]Gobatto, C. A. [UNESP]De Mello, M. A.R. [UNESP]2022-04-28T20:40:12Z2022-04-28T20:40:12Z2008-04-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article23-34Biology of Sport, v. 25, n. 1, p. 23-34, 2008.0860-021Xhttp://hdl.handle.net/11449/2251432-s2.0-42549168074Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiology of Sportinfo:eu-repo/semantics/openAccess2022-04-28T20:40:12Zoai:repositorio.unesp.br:11449/225143Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:47:26.933449Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Minimum blood lactate and muscle protein of rats during swimming exercise
title Minimum blood lactate and muscle protein of rats during swimming exercise
spellingShingle Minimum blood lactate and muscle protein of rats during swimming exercise
Voltarelli, F. A. [UNESP]
Lactate minimum test
Minimum blood lactate
Protein degradation
Protein synthesis
Rats
title_short Minimum blood lactate and muscle protein of rats during swimming exercise
title_full Minimum blood lactate and muscle protein of rats during swimming exercise
title_fullStr Minimum blood lactate and muscle protein of rats during swimming exercise
title_full_unstemmed Minimum blood lactate and muscle protein of rats during swimming exercise
title_sort Minimum blood lactate and muscle protein of rats during swimming exercise
author Voltarelli, F. A. [UNESP]
author_facet Voltarelli, F. A. [UNESP]
Gobatto, C. A. [UNESP]
De Mello, M. A.R. [UNESP]
author_role author
author2 Gobatto, C. A. [UNESP]
De Mello, M. A.R. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Voltarelli, F. A. [UNESP]
Gobatto, C. A. [UNESP]
De Mello, M. A.R. [UNESP]
dc.subject.por.fl_str_mv Lactate minimum test
Minimum blood lactate
Protein degradation
Protein synthesis
Rats
topic Lactate minimum test
Minimum blood lactate
Protein degradation
Protein synthesis
Rats
description Few studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7±0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 mm) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[14C] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4±0.01) and equivalent (6.4±0.006) to MBL but increased, progressively, with the high load. There were no differences in protein synthesis (pmol/mg.h) among rest values (65.2±3.4) and after-exercise supporting the loads below (61.5±1.3) and the equivalent (60.7±1.7) to MBL but there was a decrease with the high load (36.6±2.0). Protein breakdown rates (pmol/g.h) increase after exercise supporting the loads below (227.0±6.1), equivalent (227.9±6.0) and high (363.6±7.1) to MBL in relation to the rest (214.3±6.0). The results indicate the viability of the application of LMT in studies with rats since it detected alterations imposed by exercise.
publishDate 2008
dc.date.none.fl_str_mv 2008-04-29
2022-04-28T20:40:12Z
2022-04-28T20:40:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Biology of Sport, v. 25, n. 1, p. 23-34, 2008.
0860-021X
http://hdl.handle.net/11449/225143
2-s2.0-42549168074
identifier_str_mv Biology of Sport, v. 25, n. 1, p. 23-34, 2008.
0860-021X
2-s2.0-42549168074
url http://hdl.handle.net/11449/225143
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biology of Sport
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 23-34
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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