Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/231951 |
Resumo: | The biological control of the plants diseases, caused by fungi, is carried out using others organisms (predator, parasite or pathogen). Among the possible agents of biocontrol, a fungus has been highlighting as promising and it is known as Clonostachys rosea, asexual form of Bionectria ochroleuca. For that, it is necessary the in vitro production of spores of this fungus. In this study were tested several culture media to select those with better conidia production. The study was conducted at Plant Protection Division, in the Plant Production Department, FCA - UNESP, Botucatu campus, São Paulo state, Brazil. We used the isolated CCR64 (EMBRAPA-CNPMA). The means of crops were: BDA; Oats-Agar; Mazeina-Agar; Rice-Agar; V8-5%; V8-10%; V8-20%; TJ-5%; TJ-10, TJ-20%. The sporulation of the fungus in different culture media was estimated at 8° days after of the incubation. The data were analyzed using method of comparing averages, using the Tukey test, at 5% probability, and the data processed, using (X +1)0.5 transformation. All culture media tested were able to produce conidia. It was found that the best culture media for production of conidia of Bionectria ochroleuca is the TJ-5%, followed by TJ-20%, with an sporulation average of 3,5×106 conidia/ml. |
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Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturasEvaluation of the Bionectria ochroleuca sporulation in different culture mediaBiological controlInoculantMultiplicationThe biological control of the plants diseases, caused by fungi, is carried out using others organisms (predator, parasite or pathogen). Among the possible agents of biocontrol, a fungus has been highlighting as promising and it is known as Clonostachys rosea, asexual form of Bionectria ochroleuca. For that, it is necessary the in vitro production of spores of this fungus. In this study were tested several culture media to select those with better conidia production. The study was conducted at Plant Protection Division, in the Plant Production Department, FCA - UNESP, Botucatu campus, São Paulo state, Brazil. We used the isolated CCR64 (EMBRAPA-CNPMA). The means of crops were: BDA; Oats-Agar; Mazeina-Agar; Rice-Agar; V8-5%; V8-10%; V8-20%; TJ-5%; TJ-10, TJ-20%. The sporulation of the fungus in different culture media was estimated at 8° days after of the incubation. The data were analyzed using method of comparing averages, using the Tukey test, at 5% probability, and the data processed, using (X +1)0.5 transformation. All culture media tested were able to produce conidia. It was found that the best culture media for production of conidia of Bionectria ochroleuca is the TJ-5%, followed by TJ-20%, with an sporulation average of 3,5×106 conidia/ml.Universidade Estadual Paulista - UNESP Faculdade de Ciências Agronômicas - FCA, campus Botucatu, Botucatu, SPUniversidade Estadual Paulista - UNESP Faculdade de Ciências Agronômicas - FCA, campus Botucatu, Botucatu, SPUniversidade Estadual Paulista (UNESP)Chagas, Haroldo Antunes [UNESP]Rosa, Daniel Dias [UNESP]Basseto, Marco Antonio [UNESP]Zanotto, Mauricio Dutra [UNESP]Furtado, Edson Luiz [UNESP]2022-04-29T08:48:19Z2022-04-29T08:48:19Z2010-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article334-338Bioscience Journal, v. 26, n. 3, p. 334-338, 2010.1516-37251981-3163http://hdl.handle.net/11449/2319512-s2.0-77957366655Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporBioscience Journalinfo:eu-repo/semantics/openAccess2024-04-30T19:29:13Zoai:repositorio.unesp.br:11449/231951Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T19:29:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas Evaluation of the Bionectria ochroleuca sporulation in different culture media |
title |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas |
spellingShingle |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas Chagas, Haroldo Antunes [UNESP] Biological control Inoculant Multiplication |
title_short |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas |
title_full |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas |
title_fullStr |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas |
title_full_unstemmed |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas |
title_sort |
Avaliação da esporulação de Bionectria ochroleuca em diferentes meios de culturas |
author |
Chagas, Haroldo Antunes [UNESP] |
author_facet |
Chagas, Haroldo Antunes [UNESP] Rosa, Daniel Dias [UNESP] Basseto, Marco Antonio [UNESP] Zanotto, Mauricio Dutra [UNESP] Furtado, Edson Luiz [UNESP] |
author_role |
author |
author2 |
Rosa, Daniel Dias [UNESP] Basseto, Marco Antonio [UNESP] Zanotto, Mauricio Dutra [UNESP] Furtado, Edson Luiz [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Chagas, Haroldo Antunes [UNESP] Rosa, Daniel Dias [UNESP] Basseto, Marco Antonio [UNESP] Zanotto, Mauricio Dutra [UNESP] Furtado, Edson Luiz [UNESP] |
dc.subject.por.fl_str_mv |
Biological control Inoculant Multiplication |
topic |
Biological control Inoculant Multiplication |
description |
The biological control of the plants diseases, caused by fungi, is carried out using others organisms (predator, parasite or pathogen). Among the possible agents of biocontrol, a fungus has been highlighting as promising and it is known as Clonostachys rosea, asexual form of Bionectria ochroleuca. For that, it is necessary the in vitro production of spores of this fungus. In this study were tested several culture media to select those with better conidia production. The study was conducted at Plant Protection Division, in the Plant Production Department, FCA - UNESP, Botucatu campus, São Paulo state, Brazil. We used the isolated CCR64 (EMBRAPA-CNPMA). The means of crops were: BDA; Oats-Agar; Mazeina-Agar; Rice-Agar; V8-5%; V8-10%; V8-20%; TJ-5%; TJ-10, TJ-20%. The sporulation of the fungus in different culture media was estimated at 8° days after of the incubation. The data were analyzed using method of comparing averages, using the Tukey test, at 5% probability, and the data processed, using (X +1)0.5 transformation. All culture media tested were able to produce conidia. It was found that the best culture media for production of conidia of Bionectria ochroleuca is the TJ-5%, followed by TJ-20%, with an sporulation average of 3,5×106 conidia/ml. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05-01 2022-04-29T08:48:19Z 2022-04-29T08:48:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Bioscience Journal, v. 26, n. 3, p. 334-338, 2010. 1516-3725 1981-3163 http://hdl.handle.net/11449/231951 2-s2.0-77957366655 |
identifier_str_mv |
Bioscience Journal, v. 26, n. 3, p. 334-338, 2010. 1516-3725 1981-3163 2-s2.0-77957366655 |
url |
http://hdl.handle.net/11449/231951 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Bioscience Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
334-338 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965370881146880 |