Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00580-020-03113-z http://hdl.handle.net/11449/206710 |
Resumo: | The aim of this study was to investigate glycoconjugate immunostaining of the biotinylated lectins: UEA - Ulex europaeus (gorse), PNA - Arachis hypogaea (peanut), HPA - Helix pomatia (Roman snail), and DBA - Dolichos biflorus, in normal mammary glands (control; n = 7), mammary neoplasms (n = 111), and mammary neoplasm metastasis (n = 10). One hundred twenty-eight mammary gland tissue samples were used; 7 samples without history of neoplasms (control). The remaining samples (121) were mammary neoplasms or metastasis. In all cases (UEA, HPA, PNA, and DBA), the binding to normal epithelium was uniform. The immunohistochemical marking was in the membrane and cytoplasm of epithelial cells in most of the cases. Large variations of binding between cell membranes, cytoplasm, and nuclei occurred in the neoplastic and metastatic tissues. Though lacking statistical differences, UEA, PNA, HPA, and DBA lectins revealed different marking patterns between tissues, being metastatic foci less marked in comparison with normal and neoplastic mammary gland tissues. In addition, there were no significant differences in the immunostaining of lectins between tumors where HPA was the most expressed in papillary carcinoma I, PNA in papillary carcinoma II, DBA in carcinosarcoma, and HPA and DBA in mixed carcinoma. It was concluded that the lectins have diagnostic potential and they have been useful for differentiation between normal and neoplastic breast tissue of metastatic foci in mammary tumor in dogs. |
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Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasmsDiagnosis of neoplasmsLectin histochemistryOncologyTumorThe aim of this study was to investigate glycoconjugate immunostaining of the biotinylated lectins: UEA - Ulex europaeus (gorse), PNA - Arachis hypogaea (peanut), HPA - Helix pomatia (Roman snail), and DBA - Dolichos biflorus, in normal mammary glands (control; n = 7), mammary neoplasms (n = 111), and mammary neoplasm metastasis (n = 10). One hundred twenty-eight mammary gland tissue samples were used; 7 samples without history of neoplasms (control). The remaining samples (121) were mammary neoplasms or metastasis. In all cases (UEA, HPA, PNA, and DBA), the binding to normal epithelium was uniform. The immunohistochemical marking was in the membrane and cytoplasm of epithelial cells in most of the cases. Large variations of binding between cell membranes, cytoplasm, and nuclei occurred in the neoplastic and metastatic tissues. Though lacking statistical differences, UEA, PNA, HPA, and DBA lectins revealed different marking patterns between tissues, being metastatic foci less marked in comparison with normal and neoplastic mammary gland tissues. In addition, there were no significant differences in the immunostaining of lectins between tumors where HPA was the most expressed in papillary carcinoma I, PNA in papillary carcinoma II, DBA in carcinosarcoma, and HPA and DBA in mixed carcinoma. It was concluded that the lectins have diagnostic potential and they have been useful for differentiation between normal and neoplastic breast tissue of metastatic foci in mammary tumor in dogs.Course of Veterinary Medicine Centro Universitário de Mineiros (Unifimes), MineirosFederal University of Jequitinhonha and Mucuri Valleys (UFVJM)Institute of Biodiversity and Forest Federal University of Western Pará (UFOPA)Instituto del Mar del Perú IMARPECourse of Veterinary Medicine University Center Ingá (UNINGÁ), MaringáDepartment of Animal Pathology Paulista State University (UNESP), JaboticabalDepartment of Animal Pathology Paulista State University (UNESP), JaboticabalCentro Universitário de Mineiros (Unifimes)Universidade Federal de Viçosa (UFV)Federal University of Western Pará (UFOPA)IMARPEUniversity Center Ingá (UNINGÁ)Universidade Estadual Paulista (Unesp)Bezerril, Juliana E.Marcusso, Paulo F.Claudiano, Gustavo S.Yunis-Aguinaga, JeffersonPetrillo, Thalita R.Alessi, Antonio C. [UNESP]2021-06-25T10:36:54Z2021-06-25T10:36:54Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1091-1097http://dx.doi.org/10.1007/s00580-020-03113-zComparative Clinical Pathology, v. 29, n. 6, p. 1091-1097, 2020.1618-565X1618-5641http://hdl.handle.net/11449/20671010.1007/s00580-020-03113-z2-s2.0-85093115892Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Clinical Pathologyinfo:eu-repo/semantics/openAccess2024-06-07T13:01:55Zoai:repositorio.unesp.br:11449/206710Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:25:04.107942Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms |
title |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms |
spellingShingle |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms Bezerril, Juliana E. Diagnosis of neoplasms Lectin histochemistry Oncology Tumor |
title_short |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms |
title_full |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms |
title_fullStr |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms |
title_full_unstemmed |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms |
title_sort |
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms |
author |
Bezerril, Juliana E. |
author_facet |
Bezerril, Juliana E. Marcusso, Paulo F. Claudiano, Gustavo S. Yunis-Aguinaga, Jefferson Petrillo, Thalita R. Alessi, Antonio C. [UNESP] |
author_role |
author |
author2 |
Marcusso, Paulo F. Claudiano, Gustavo S. Yunis-Aguinaga, Jefferson Petrillo, Thalita R. Alessi, Antonio C. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Centro Universitário de Mineiros (Unifimes) Universidade Federal de Viçosa (UFV) Federal University of Western Pará (UFOPA) IMARPE University Center Ingá (UNINGÁ) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bezerril, Juliana E. Marcusso, Paulo F. Claudiano, Gustavo S. Yunis-Aguinaga, Jefferson Petrillo, Thalita R. Alessi, Antonio C. [UNESP] |
dc.subject.por.fl_str_mv |
Diagnosis of neoplasms Lectin histochemistry Oncology Tumor |
topic |
Diagnosis of neoplasms Lectin histochemistry Oncology Tumor |
description |
The aim of this study was to investigate glycoconjugate immunostaining of the biotinylated lectins: UEA - Ulex europaeus (gorse), PNA - Arachis hypogaea (peanut), HPA - Helix pomatia (Roman snail), and DBA - Dolichos biflorus, in normal mammary glands (control; n = 7), mammary neoplasms (n = 111), and mammary neoplasm metastasis (n = 10). One hundred twenty-eight mammary gland tissue samples were used; 7 samples without history of neoplasms (control). The remaining samples (121) were mammary neoplasms or metastasis. In all cases (UEA, HPA, PNA, and DBA), the binding to normal epithelium was uniform. The immunohistochemical marking was in the membrane and cytoplasm of epithelial cells in most of the cases. Large variations of binding between cell membranes, cytoplasm, and nuclei occurred in the neoplastic and metastatic tissues. Though lacking statistical differences, UEA, PNA, HPA, and DBA lectins revealed different marking patterns between tissues, being metastatic foci less marked in comparison with normal and neoplastic mammary gland tissues. In addition, there were no significant differences in the immunostaining of lectins between tumors where HPA was the most expressed in papillary carcinoma I, PNA in papillary carcinoma II, DBA in carcinosarcoma, and HPA and DBA in mixed carcinoma. It was concluded that the lectins have diagnostic potential and they have been useful for differentiation between normal and neoplastic breast tissue of metastatic foci in mammary tumor in dogs. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T10:36:54Z 2021-06-25T10:36:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00580-020-03113-z Comparative Clinical Pathology, v. 29, n. 6, p. 1091-1097, 2020. 1618-565X 1618-5641 http://hdl.handle.net/11449/206710 10.1007/s00580-020-03113-z 2-s2.0-85093115892 |
url |
http://dx.doi.org/10.1007/s00580-020-03113-z http://hdl.handle.net/11449/206710 |
identifier_str_mv |
Comparative Clinical Pathology, v. 29, n. 6, p. 1091-1097, 2020. 1618-565X 1618-5641 10.1007/s00580-020-03113-z 2-s2.0-85093115892 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Clinical Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1091-1097 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128647302742016 |