Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2022.103219 http://hdl.handle.net/11449/249535 |
Resumo: | In our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms’ viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log10 GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log10 GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations. |
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Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitisChlorin e6PeriodontitisPhotodynamic therapyPhotodynamic TherapyPhotosensitizing agentsIn our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms’ viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log10 GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log10 GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations.Department of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Chemistry Federal University of São Carlos (UFSCar), SPDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPUniversidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Garcia de Carvalho, Gabriel [UNESP]Pacheco Mateo, Rafaela [UNESP]Costa e Silva, RodrigoMaquera Huacho, Patricia Milagros [UNESP]de Souza Rastelli, Alessandra Nara [UNESP]de Oliveira, Kleber ThiagoChierici Marcantonio, Rosemary Adriana [UNESP]Zandim-Barcelos, Daniela Leal [UNESP]Palomari Spolidorio, Denise Madalena [UNESP]2023-07-29T16:02:23Z2023-07-29T16:02:23Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2022.103219Photodiagnosis and Photodynamic Therapy, v. 41.1873-15971572-1000http://hdl.handle.net/11449/24953510.1016/j.pdpdt.2022.1032192-s2.0-85145744023Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2023-07-29T16:02:23Zoai:repositorio.unesp.br:11449/249535Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:02:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis |
title |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis |
spellingShingle |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis Garcia de Carvalho, Gabriel [UNESP] Chlorin e6 Periodontitis Photodynamic therapy Photodynamic Therapy Photosensitizing agents |
title_short |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis |
title_full |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis |
title_fullStr |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis |
title_full_unstemmed |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis |
title_sort |
Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis |
author |
Garcia de Carvalho, Gabriel [UNESP] |
author_facet |
Garcia de Carvalho, Gabriel [UNESP] Pacheco Mateo, Rafaela [UNESP] Costa e Silva, Rodrigo Maquera Huacho, Patricia Milagros [UNESP] de Souza Rastelli, Alessandra Nara [UNESP] de Oliveira, Kleber Thiago Chierici Marcantonio, Rosemary Adriana [UNESP] Zandim-Barcelos, Daniela Leal [UNESP] Palomari Spolidorio, Denise Madalena [UNESP] |
author_role |
author |
author2 |
Pacheco Mateo, Rafaela [UNESP] Costa e Silva, Rodrigo Maquera Huacho, Patricia Milagros [UNESP] de Souza Rastelli, Alessandra Nara [UNESP] de Oliveira, Kleber Thiago Chierici Marcantonio, Rosemary Adriana [UNESP] Zandim-Barcelos, Daniela Leal [UNESP] Palomari Spolidorio, Denise Madalena [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Garcia de Carvalho, Gabriel [UNESP] Pacheco Mateo, Rafaela [UNESP] Costa e Silva, Rodrigo Maquera Huacho, Patricia Milagros [UNESP] de Souza Rastelli, Alessandra Nara [UNESP] de Oliveira, Kleber Thiago Chierici Marcantonio, Rosemary Adriana [UNESP] Zandim-Barcelos, Daniela Leal [UNESP] Palomari Spolidorio, Denise Madalena [UNESP] |
dc.subject.por.fl_str_mv |
Chlorin e6 Periodontitis Photodynamic therapy Photodynamic Therapy Photosensitizing agents |
topic |
Chlorin e6 Periodontitis Photodynamic therapy Photodynamic Therapy Photosensitizing agents |
description |
In our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms’ viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log10 GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log10 GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:02:23Z 2023-07-29T16:02:23Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2022.103219 Photodiagnosis and Photodynamic Therapy, v. 41. 1873-1597 1572-1000 http://hdl.handle.net/11449/249535 10.1016/j.pdpdt.2022.103219 2-s2.0-85145744023 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2022.103219 http://hdl.handle.net/11449/249535 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 41. 1873-1597 1572-1000 10.1016/j.pdpdt.2022.103219 2-s2.0-85145744023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965228184633344 |