Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis

Detalhes bibliográficos
Autor(a) principal: Garcia de Carvalho, Gabriel [UNESP]
Data de Publicação: 2023
Outros Autores: Pacheco Mateo, Rafaela [UNESP], Costa e Silva, Rodrigo, Maquera Huacho, Patricia Milagros [UNESP], de Souza Rastelli, Alessandra Nara [UNESP], de Oliveira, Kleber Thiago, Chierici Marcantonio, Rosemary Adriana [UNESP], Zandim-Barcelos, Daniela Leal [UNESP], Palomari Spolidorio, Denise Madalena [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2022.103219
http://hdl.handle.net/11449/249535
Resumo: In our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms’ viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log10 GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log10 GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations.
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spelling Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitisChlorin e6PeriodontitisPhotodynamic therapyPhotodynamic TherapyPhotosensitizing agentsIn our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms’ viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log10 GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log10 GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations.Department of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Chemistry Federal University of São Carlos (UFSCar), SPDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680, SPUniversidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Garcia de Carvalho, Gabriel [UNESP]Pacheco Mateo, Rafaela [UNESP]Costa e Silva, RodrigoMaquera Huacho, Patricia Milagros [UNESP]de Souza Rastelli, Alessandra Nara [UNESP]de Oliveira, Kleber ThiagoChierici Marcantonio, Rosemary Adriana [UNESP]Zandim-Barcelos, Daniela Leal [UNESP]Palomari Spolidorio, Denise Madalena [UNESP]2023-07-29T16:02:23Z2023-07-29T16:02:23Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2022.103219Photodiagnosis and Photodynamic Therapy, v. 41.1873-15971572-1000http://hdl.handle.net/11449/24953510.1016/j.pdpdt.2022.1032192-s2.0-85145744023Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2023-07-29T16:02:23Zoai:repositorio.unesp.br:11449/249535Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:02:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
title Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
spellingShingle Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
Garcia de Carvalho, Gabriel [UNESP]
Chlorin e6
Periodontitis
Photodynamic therapy
Photodynamic Therapy
Photosensitizing agents
title_short Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
title_full Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
title_fullStr Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
title_full_unstemmed Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
title_sort Chlorin-based photosensitizer under blue or red-light irradiation against multi-species biofilms related to periodontitis
author Garcia de Carvalho, Gabriel [UNESP]
author_facet Garcia de Carvalho, Gabriel [UNESP]
Pacheco Mateo, Rafaela [UNESP]
Costa e Silva, Rodrigo
Maquera Huacho, Patricia Milagros [UNESP]
de Souza Rastelli, Alessandra Nara [UNESP]
de Oliveira, Kleber Thiago
Chierici Marcantonio, Rosemary Adriana [UNESP]
Zandim-Barcelos, Daniela Leal [UNESP]
Palomari Spolidorio, Denise Madalena [UNESP]
author_role author
author2 Pacheco Mateo, Rafaela [UNESP]
Costa e Silva, Rodrigo
Maquera Huacho, Patricia Milagros [UNESP]
de Souza Rastelli, Alessandra Nara [UNESP]
de Oliveira, Kleber Thiago
Chierici Marcantonio, Rosemary Adriana [UNESP]
Zandim-Barcelos, Daniela Leal [UNESP]
Palomari Spolidorio, Denise Madalena [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Garcia de Carvalho, Gabriel [UNESP]
Pacheco Mateo, Rafaela [UNESP]
Costa e Silva, Rodrigo
Maquera Huacho, Patricia Milagros [UNESP]
de Souza Rastelli, Alessandra Nara [UNESP]
de Oliveira, Kleber Thiago
Chierici Marcantonio, Rosemary Adriana [UNESP]
Zandim-Barcelos, Daniela Leal [UNESP]
Palomari Spolidorio, Denise Madalena [UNESP]
dc.subject.por.fl_str_mv Chlorin e6
Periodontitis
Photodynamic therapy
Photodynamic Therapy
Photosensitizing agents
topic Chlorin e6
Periodontitis
Photodynamic therapy
Photodynamic Therapy
Photosensitizing agents
description In our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms’ viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log10 GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log10 GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:02:23Z
2023-07-29T16:02:23Z
2023-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2022.103219
Photodiagnosis and Photodynamic Therapy, v. 41.
1873-1597
1572-1000
http://hdl.handle.net/11449/249535
10.1016/j.pdpdt.2022.103219
2-s2.0-85145744023
url http://dx.doi.org/10.1016/j.pdpdt.2022.103219
http://hdl.handle.net/11449/249535
identifier_str_mv Photodiagnosis and Photodynamic Therapy, v. 41.
1873-1597
1572-1000
10.1016/j.pdpdt.2022.103219
2-s2.0-85145744023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis and Photodynamic Therapy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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