Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbrc.2020.01.025 http://hdl.handle.net/11449/201466 |
Resumo: | Although studies have focused on extracellular matrix (ECM) remodeling during the formation and functioning of adult ovaries, there is no comprehensive understanding of the mechanisms controlling preantral follicle development in fetal bovine ovaries during gestation. Thus, to gain insights into ECM remodeling during initial ovarian development, we used fetal ovaries to quantify the fractal dimension (FD), total collagen, and relative mRNA abundance of genes related to ECM remodeling (COL1A1, COL1A2, COL4A1, MMP2, MMP9, MMP14, TIMP1, and TIMP2). For this, pairs of fetal ovaries were obtained from cows in a local abattoir at days 60, 90, 120, and 150 of gestation; one of each pair was submitted to RNA extraction for target transcript analysis, and the other was used for total collagen and FD evaluation. From day 120 total collagen appeared to occupy a greater area in the fetal ovary. The fractal analysis with picrosirius red staining shows higher at day 150 when compared with that on day 60. On the contrary, we found an inverse pattern when we used the hematoxylin and eosin staining approach. Concerning target gene expression, the relative abundances of COL1A1, COL4A1, MMP2, MMP14, TIMP1, and TIMP2 mRNA were higher on day 150 when compared with that on day 60. We conclude that fractal analysis reflects the morphological changes occurring during structural organization of the fetal ovary and that the expression of genes related to ECM remodeling is modulated throughout gestation in bovine fetal ovaries. |
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Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovaryCattleCollagenGene expressionMetalloproteinasesPicrosiriusPreantral folliclesAlthough studies have focused on extracellular matrix (ECM) remodeling during the formation and functioning of adult ovaries, there is no comprehensive understanding of the mechanisms controlling preantral follicle development in fetal bovine ovaries during gestation. Thus, to gain insights into ECM remodeling during initial ovarian development, we used fetal ovaries to quantify the fractal dimension (FD), total collagen, and relative mRNA abundance of genes related to ECM remodeling (COL1A1, COL1A2, COL4A1, MMP2, MMP9, MMP14, TIMP1, and TIMP2). For this, pairs of fetal ovaries were obtained from cows in a local abattoir at days 60, 90, 120, and 150 of gestation; one of each pair was submitted to RNA extraction for target transcript analysis, and the other was used for total collagen and FD evaluation. From day 120 total collagen appeared to occupy a greater area in the fetal ovary. The fractal analysis with picrosirius red staining shows higher at day 150 when compared with that on day 60. On the contrary, we found an inverse pattern when we used the hematoxylin and eosin staining approach. Concerning target gene expression, the relative abundances of COL1A1, COL4A1, MMP2, MMP14, TIMP1, and TIMP2 mRNA were higher on day 150 when compared with that on day 60. We conclude that fractal analysis reflects the morphological changes occurring during structural organization of the fetal ovary and that the expression of genes related to ECM remodeling is modulated throughout gestation in bovine fetal ovaries.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)University of Western São Paulo (Unoeste)Department of Pharmacology Institute of Biosciences São Paulo State University (Unesp)Department of Morphology Institute of Biosciences São Paulo State University (Unesp), São PauloDepartment of Pharmacology Institute of Biosciences São Paulo State University (Unesp)Department of Morphology Institute of Biosciences São Paulo State University (Unesp), São PauloCAPES: 001FAPESP: 2013/11480-3FAPESP: 2015/04505-5FAPESP: 2018/06674-7University of Western São Paulo (Unoeste)Universidade Estadual Paulista (Unesp)Franchi, Fernanda Fagali [UNESP]Hernandes, Mariana PelichoCoalho Ferreira, Amanda LeticiaVieira de Lima, Victor Augustode Oliveira Mendes, LeonardoMusa de Aquino, Ariana [UNESP]Scarano, Wellerson Rodrigo [UNESP]César de Souza Castilho, Anthony2020-12-12T02:33:14Z2020-12-12T02:33:14Z2020-03-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article823-828http://dx.doi.org/10.1016/j.bbrc.2020.01.025Biochemical and Biophysical Research Communications, v. 523, n. 4, p. 823-828, 2020.1090-21040006-291Xhttp://hdl.handle.net/11449/20146610.1016/j.bbrc.2020.01.0252-s2.0-85077742538Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemical and Biophysical Research Communicationsinfo:eu-repo/semantics/openAccess2021-10-22T19:45:02Zoai:repositorio.unesp.br:11449/201466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:06:08.254614Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary |
title |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary |
spellingShingle |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary Franchi, Fernanda Fagali [UNESP] Cattle Collagen Gene expression Metalloproteinases Picrosirius Preantral follicles |
title_short |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary |
title_full |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary |
title_fullStr |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary |
title_full_unstemmed |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary |
title_sort |
Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary |
author |
Franchi, Fernanda Fagali [UNESP] |
author_facet |
Franchi, Fernanda Fagali [UNESP] Hernandes, Mariana Pelicho Coalho Ferreira, Amanda Leticia Vieira de Lima, Victor Augusto de Oliveira Mendes, Leonardo Musa de Aquino, Ariana [UNESP] Scarano, Wellerson Rodrigo [UNESP] César de Souza Castilho, Anthony |
author_role |
author |
author2 |
Hernandes, Mariana Pelicho Coalho Ferreira, Amanda Leticia Vieira de Lima, Victor Augusto de Oliveira Mendes, Leonardo Musa de Aquino, Ariana [UNESP] Scarano, Wellerson Rodrigo [UNESP] César de Souza Castilho, Anthony |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Western São Paulo (Unoeste) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Franchi, Fernanda Fagali [UNESP] Hernandes, Mariana Pelicho Coalho Ferreira, Amanda Leticia Vieira de Lima, Victor Augusto de Oliveira Mendes, Leonardo Musa de Aquino, Ariana [UNESP] Scarano, Wellerson Rodrigo [UNESP] César de Souza Castilho, Anthony |
dc.subject.por.fl_str_mv |
Cattle Collagen Gene expression Metalloproteinases Picrosirius Preantral follicles |
topic |
Cattle Collagen Gene expression Metalloproteinases Picrosirius Preantral follicles |
description |
Although studies have focused on extracellular matrix (ECM) remodeling during the formation and functioning of adult ovaries, there is no comprehensive understanding of the mechanisms controlling preantral follicle development in fetal bovine ovaries during gestation. Thus, to gain insights into ECM remodeling during initial ovarian development, we used fetal ovaries to quantify the fractal dimension (FD), total collagen, and relative mRNA abundance of genes related to ECM remodeling (COL1A1, COL1A2, COL4A1, MMP2, MMP9, MMP14, TIMP1, and TIMP2). For this, pairs of fetal ovaries were obtained from cows in a local abattoir at days 60, 90, 120, and 150 of gestation; one of each pair was submitted to RNA extraction for target transcript analysis, and the other was used for total collagen and FD evaluation. From day 120 total collagen appeared to occupy a greater area in the fetal ovary. The fractal analysis with picrosirius red staining shows higher at day 150 when compared with that on day 60. On the contrary, we found an inverse pattern when we used the hematoxylin and eosin staining approach. Concerning target gene expression, the relative abundances of COL1A1, COL4A1, MMP2, MMP14, TIMP1, and TIMP2 mRNA were higher on day 150 when compared with that on day 60. We conclude that fractal analysis reflects the morphological changes occurring during structural organization of the fetal ovary and that the expression of genes related to ECM remodeling is modulated throughout gestation in bovine fetal ovaries. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:33:14Z 2020-12-12T02:33:14Z 2020-03-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbrc.2020.01.025 Biochemical and Biophysical Research Communications, v. 523, n. 4, p. 823-828, 2020. 1090-2104 0006-291X http://hdl.handle.net/11449/201466 10.1016/j.bbrc.2020.01.025 2-s2.0-85077742538 |
url |
http://dx.doi.org/10.1016/j.bbrc.2020.01.025 http://hdl.handle.net/11449/201466 |
identifier_str_mv |
Biochemical and Biophysical Research Communications, v. 523, n. 4, p. 823-828, 2020. 1090-2104 0006-291X 10.1016/j.bbrc.2020.01.025 2-s2.0-85077742538 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical and Biophysical Research Communications |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
823-828 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128236768460800 |