Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-019-41312-z http://hdl.handle.net/11449/190200 |
Resumo: | Acute-phase protein (APPs) serum levels have been studied in many human diseases, and their components contribute to host defense during the evolution of infectious diseases by acting as part of the innate immune system. Based on the importance of establishing new experimental models, the present investigation evaluated the modulation of APPs following inflammatory stimulus by the inoculation of Aeromonas hydrophila in tilapias. Fish were sampled 6 and 24 hours post-infection. Tilapias presented increase of positive APPs such as ceruloplasmin, haptoglobin, alpha-2-macroglobulin and complement C3, as well as decrease of negative APPs such as albumin and transferrin. The protein response of tilapias during the course of bacterial infection showed correlation with the kinetics of cellular accumulation in the inflamed focus with significant increase of granulocytes, thrombocytes, lymphocytes and macrophages. However, granulocytes were the predominant cells, associated with increment in the reactive oxygen species (ROS) production. Showing responses similar to those observed in humans, the modulation of APPs and the kinetics of cellular accumulation in the exudate demonstrate the feasibility of this alternative experimental model for advances and studies to understand changes in pathophysiological mechanisms of acute inflammatory reaction due to bacterial infection. |
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Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-modelAcute-phase protein (APPs) serum levels have been studied in many human diseases, and their components contribute to host defense during the evolution of infectious diseases by acting as part of the innate immune system. Based on the importance of establishing new experimental models, the present investigation evaluated the modulation of APPs following inflammatory stimulus by the inoculation of Aeromonas hydrophila in tilapias. Fish were sampled 6 and 24 hours post-infection. Tilapias presented increase of positive APPs such as ceruloplasmin, haptoglobin, alpha-2-macroglobulin and complement C3, as well as decrease of negative APPs such as albumin and transferrin. The protein response of tilapias during the course of bacterial infection showed correlation with the kinetics of cellular accumulation in the inflamed focus with significant increase of granulocytes, thrombocytes, lymphocytes and macrophages. However, granulocytes were the predominant cells, associated with increment in the reactive oxygen species (ROS) production. Showing responses similar to those observed in humans, the modulation of APPs and the kinetics of cellular accumulation in the exudate demonstrate the feasibility of this alternative experimental model for advances and studies to understand changes in pathophysiological mechanisms of acute inflammatory reaction due to bacterial infection.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Preventive Veterinary Medicine São Paulo State University (UNESP)Department of Pharmacology and Morphology ICB-UFMGImmunoregulation Unit of the Special Laboratory of Applied Toxicology (CEPID/FAPESP) Butantan InstituteLaboratório de Bioquímica de Peptídeos UNIFESPVeterinary College Federal University of RondoniaLaboratory of Animal Pharmacology and Toxicology Brasil UniversityDepartment of Preventive Veterinary Medicine São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Butantan InstituteUniversidade Federal de São Paulo (UNIFESP)Federal University of RondoniaBrasil UniversityCharlie-Silva, Ives [UNESP]Klein, AndreGomes, Juliana M. M.Prado, Ed J. R. [UNESP]Moraes, Alessandra C. [UNESP]Eto, Silas F. [UNESP]Fernandes, Dayanne C. [UNESP]Fagliari, José J. [UNESP]Junior, José D. CorrêaLima, CarlaLopes-Ferreira, MônicaConceição, KatiaManrique, Wilson G.Belo, Marco A. A. [UNESP]2019-10-06T17:05:31Z2019-10-06T17:05:31Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-019-41312-zScientific Reports, v. 9, n. 1, 2019.2045-2322http://hdl.handle.net/11449/19020010.1038/s41598-019-41312-z2-s2.0-85063055485Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-06-06T14:10:35Zoai:repositorio.unesp.br:11449/190200Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-06-06T14:10:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model |
title |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model |
spellingShingle |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model Charlie-Silva, Ives [UNESP] |
title_short |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model |
title_full |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model |
title_fullStr |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model |
title_full_unstemmed |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model |
title_sort |
Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model |
author |
Charlie-Silva, Ives [UNESP] |
author_facet |
Charlie-Silva, Ives [UNESP] Klein, Andre Gomes, Juliana M. M. Prado, Ed J. R. [UNESP] Moraes, Alessandra C. [UNESP] Eto, Silas F. [UNESP] Fernandes, Dayanne C. [UNESP] Fagliari, José J. [UNESP] Junior, José D. Corrêa Lima, Carla Lopes-Ferreira, Mônica Conceição, Katia Manrique, Wilson G. Belo, Marco A. A. [UNESP] |
author_role |
author |
author2 |
Klein, Andre Gomes, Juliana M. M. Prado, Ed J. R. [UNESP] Moraes, Alessandra C. [UNESP] Eto, Silas F. [UNESP] Fernandes, Dayanne C. [UNESP] Fagliari, José J. [UNESP] Junior, José D. Corrêa Lima, Carla Lopes-Ferreira, Mônica Conceição, Katia Manrique, Wilson G. Belo, Marco A. A. [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) Butantan Institute Universidade Federal de São Paulo (UNIFESP) Federal University of Rondonia Brasil University |
dc.contributor.author.fl_str_mv |
Charlie-Silva, Ives [UNESP] Klein, Andre Gomes, Juliana M. M. Prado, Ed J. R. [UNESP] Moraes, Alessandra C. [UNESP] Eto, Silas F. [UNESP] Fernandes, Dayanne C. [UNESP] Fagliari, José J. [UNESP] Junior, José D. Corrêa Lima, Carla Lopes-Ferreira, Mônica Conceição, Katia Manrique, Wilson G. Belo, Marco A. A. [UNESP] |
description |
Acute-phase protein (APPs) serum levels have been studied in many human diseases, and their components contribute to host defense during the evolution of infectious diseases by acting as part of the innate immune system. Based on the importance of establishing new experimental models, the present investigation evaluated the modulation of APPs following inflammatory stimulus by the inoculation of Aeromonas hydrophila in tilapias. Fish were sampled 6 and 24 hours post-infection. Tilapias presented increase of positive APPs such as ceruloplasmin, haptoglobin, alpha-2-macroglobulin and complement C3, as well as decrease of negative APPs such as albumin and transferrin. The protein response of tilapias during the course of bacterial infection showed correlation with the kinetics of cellular accumulation in the inflamed focus with significant increase of granulocytes, thrombocytes, lymphocytes and macrophages. However, granulocytes were the predominant cells, associated with increment in the reactive oxygen species (ROS) production. Showing responses similar to those observed in humans, the modulation of APPs and the kinetics of cellular accumulation in the exudate demonstrate the feasibility of this alternative experimental model for advances and studies to understand changes in pathophysiological mechanisms of acute inflammatory reaction due to bacterial infection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:05:31Z 2019-10-06T17:05:31Z 2019-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-019-41312-z Scientific Reports, v. 9, n. 1, 2019. 2045-2322 http://hdl.handle.net/11449/190200 10.1038/s41598-019-41312-z 2-s2.0-85063055485 |
url |
http://dx.doi.org/10.1038/s41598-019-41312-z http://hdl.handle.net/11449/190200 |
identifier_str_mv |
Scientific Reports, v. 9, n. 1, 2019. 2045-2322 10.1038/s41598-019-41312-z 2-s2.0-85063055485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1826304327275249664 |