Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats

Detalhes bibliográficos
Autor(a) principal: Fontana, Carla Raquel [UNESP]
Data de Publicação: 2018
Outros Autores: Grecco, Clovis, Bagnato, Vanderlei Salvador, de Freitas, Laura Marise [UNESP], Boussios, Constantinos I., Soukos, Nikolaos S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/cre2.98
http://hdl.handle.net/11449/170677
Resumo: The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature-induced periodontitis model was used in 30 rats. Twenty-seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease-associated microbiota.
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spelling Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in ratsbiofilm, periodontal pathogensmolecular diagnosticssampling techniqueThe prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature-induced periodontitis model was used in 30 rats. Twenty-seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease-associated microbiota.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista (Unesp) Faculdade de Ciências FarmacêuticasInstituto de Física-Grupo de Óptica–Universidade de Sao Paulo USPLaboratory for Information and Decision Systems Massachusetts Institute of Technology MITDepartment of Physics 111 Dana Research CenterUniversidade Estadual Paulista (Unesp) Faculdade de Ciências FarmacêuticasFAPESP: 2016/05345-4Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)MIT111 Dana Research CenterFontana, Carla Raquel [UNESP]Grecco, ClovisBagnato, Vanderlei Salvadorde Freitas, Laura Marise [UNESP]Boussios, Constantinos I.Soukos, Nikolaos S.2018-12-11T16:51:57Z2018-12-11T16:51:57Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19-24application/pdfhttp://dx.doi.org/10.1002/cre2.98Clinical and Experimental Dental Research, v. 4, n. 1, p. 19-24, 2018.2057-4347http://hdl.handle.net/11449/17067710.1002/cre2.982-s2.0-850421509742-s2.0-85042150974.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical and Experimental Dental Research0,167info:eu-repo/semantics/openAccess2023-12-03T06:13:38Zoai:repositorio.unesp.br:11449/170677Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:22:36.960301Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
title Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
spellingShingle Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
Fontana, Carla Raquel [UNESP]
biofilm, periodontal pathogens
molecular diagnostics
sampling technique
title_short Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
title_full Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
title_fullStr Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
title_full_unstemmed Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
title_sort Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats
author Fontana, Carla Raquel [UNESP]
author_facet Fontana, Carla Raquel [UNESP]
Grecco, Clovis
Bagnato, Vanderlei Salvador
de Freitas, Laura Marise [UNESP]
Boussios, Constantinos I.
Soukos, Nikolaos S.
author_role author
author2 Grecco, Clovis
Bagnato, Vanderlei Salvador
de Freitas, Laura Marise [UNESP]
Boussios, Constantinos I.
Soukos, Nikolaos S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
MIT
111 Dana Research Center
dc.contributor.author.fl_str_mv Fontana, Carla Raquel [UNESP]
Grecco, Clovis
Bagnato, Vanderlei Salvador
de Freitas, Laura Marise [UNESP]
Boussios, Constantinos I.
Soukos, Nikolaos S.
dc.subject.por.fl_str_mv biofilm, periodontal pathogens
molecular diagnostics
sampling technique
topic biofilm, periodontal pathogens
molecular diagnostics
sampling technique
description The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature-induced periodontitis model was used in 30 rats. Twenty-seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease-associated microbiota.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:51:57Z
2018-12-11T16:51:57Z
2018-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/cre2.98
Clinical and Experimental Dental Research, v. 4, n. 1, p. 19-24, 2018.
2057-4347
http://hdl.handle.net/11449/170677
10.1002/cre2.98
2-s2.0-85042150974
2-s2.0-85042150974.pdf
url http://dx.doi.org/10.1002/cre2.98
http://hdl.handle.net/11449/170677
identifier_str_mv Clinical and Experimental Dental Research, v. 4, n. 1, p. 19-24, 2018.
2057-4347
10.1002/cre2.98
2-s2.0-85042150974
2-s2.0-85042150974.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical and Experimental Dental Research
0,167
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19-24
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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