Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2015.09.021 http://hdl.handle.net/11449/160991 |
Resumo: | The digestible amino acid (AA) determination of ingredients, or experimental diets, are essential in obtaining the real levels of AA that are being studied, as well as of other indispensable AA, because the lack of a single indispensable AA may limit pig growth performance. Two experiments were conducted to determine the optimum levels and the ideal ratio of standardized ileal digestible (SID) Trp and Lys for barrows from 15 to 30 kg of live weight. The first experiment (Exp. 1) was conducted to determine the SID AA content of the basal diet used in the growth performance study (Exp. 2). Ten crossbred barrows (21.5 +/- 1.6 kg live weight) were used in Exp. 1. Pigs were fed a basal diet and a protein free diet to determine the SID of Trp, Lys, and all other indispensable AA of the basal diet. In Exp. 2, 64 barrows (15.0 +/- 1.6 kg) were allotted to 16 diets in a 4 x 4 factorial arrangement of treatments (1.55, 1.85, 2.15, and 2.45 g SID Trp and 9.72, 11.12, 12.52, and 13.92 g SID Lys/kg) with 4 replications per treatment In both Exp. 1 and 2, each pig was considered as an experimental unit. Growth performance, Longissimus muscle (LM), backfat thickness, and plasma urea nitrogen (PUN) were evaluated. Average daily feed intake and average daily gain increased linearly (P < 0.05) with increasing levels of SID Trp. There was a quadratic effect (P=0.04) of dietary SID Trp and Lys levels on the gain:feed, and the optimal levels were estimated to be 2.1 (0.65 g/Mcal ME) and 11.98 g/kg (3.71 g/Mcal ME), respectively, resulting in a SID Trp:Lys of 17.5:100. A quadratic effect of SID Trp (P=0.021) and Lys (P=0.026) levels on LM was observed, and the optimum levels were 2.08 and 11.36 g/kg diet, respectively, with a Trp:Lys of 183:100. The amount of PUN decreased linearly (P=0.004) as SID Lys increased. For pigs used in our study (between 15 and 30 kg live weight), the SID Trp and SID Lys requirements are 2.10 and 11.98 g/kg, respectively. The corresponding Trp:Lys are 17.5:100. (C) 2015 Elsevier B.V. All rights reserved. |
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Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weightLysinePigsResponse surfaceRequirementsStandardized ileal amino acid digestibilityTryptophanThe digestible amino acid (AA) determination of ingredients, or experimental diets, are essential in obtaining the real levels of AA that are being studied, as well as of other indispensable AA, because the lack of a single indispensable AA may limit pig growth performance. Two experiments were conducted to determine the optimum levels and the ideal ratio of standardized ileal digestible (SID) Trp and Lys for barrows from 15 to 30 kg of live weight. The first experiment (Exp. 1) was conducted to determine the SID AA content of the basal diet used in the growth performance study (Exp. 2). Ten crossbred barrows (21.5 +/- 1.6 kg live weight) were used in Exp. 1. Pigs were fed a basal diet and a protein free diet to determine the SID of Trp, Lys, and all other indispensable AA of the basal diet. In Exp. 2, 64 barrows (15.0 +/- 1.6 kg) were allotted to 16 diets in a 4 x 4 factorial arrangement of treatments (1.55, 1.85, 2.15, and 2.45 g SID Trp and 9.72, 11.12, 12.52, and 13.92 g SID Lys/kg) with 4 replications per treatment In both Exp. 1 and 2, each pig was considered as an experimental unit. Growth performance, Longissimus muscle (LM), backfat thickness, and plasma urea nitrogen (PUN) were evaluated. Average daily feed intake and average daily gain increased linearly (P < 0.05) with increasing levels of SID Trp. There was a quadratic effect (P=0.04) of dietary SID Trp and Lys levels on the gain:feed, and the optimal levels were estimated to be 2.1 (0.65 g/Mcal ME) and 11.98 g/kg (3.71 g/Mcal ME), respectively, resulting in a SID Trp:Lys of 17.5:100. A quadratic effect of SID Trp (P=0.021) and Lys (P=0.026) levels on LM was observed, and the optimum levels were 2.08 and 11.36 g/kg diet, respectively, with a Trp:Lys of 183:100. The amount of PUN decreased linearly (P=0.004) as SID Lys increased. For pigs used in our study (between 15 and 30 kg live weight), the SID Trp and SID Lys requirements are 2.10 and 11.98 g/kg, respectively. The corresponding Trp:Lys are 17.5:100. (C) 2015 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Mato Grosso Sul, Dept Zootecnia, BR-79200000 Aquidauana, MS, BrazilUniv Estadual Maringa, Dept Zootecnia, BR-87020900 Maringa, Parana, BrazilUniv Tecnol Fed Parana, Dept Zootecnia, BR-85660000 Dois Vizinhos, PR, BrazilUniv Estadual Paulista, Dept Zootecnia, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Oeste Parana, Dept Zootecnia, BR-85960000 Marechal Candido Rodondo, PR, BrazilN Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USAUniv Estadual Paulista, Dept Zootecnia, BR-14884900 Jaboticabal, SP, BrazilElsevier B.V.Universidade Estadual de Mato Grosso do Sul (UEMS)Universidade Estadual de Maringá (UEM)Univ Tecnol Fed ParanaUniversidade Estadual Paulista (Unesp)Univ Estadual Oeste ParanaN Carolina State UnivPasquetti, T. J.Pozza, P. C.Moreira, I.Santos, T. C.Diaz-Huepa, L. M.Castilha, L. D.Perondi, D. [UNESP]Carvalho, P. L. O.Kim, S. W.2018-11-26T16:18:43Z2018-11-26T16:18:43Z2015-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article114-120application/pdfhttp://dx.doi.org/10.1016/j.livsci.2015.09.021Livestock Science. Amsterdam: Elsevier Science Bv, v. 181, p. 114-120, 2015.1871-1413http://hdl.handle.net/11449/16099110.1016/j.livsci.2015.09.021WOS:000365362800018WOS:000365362800018.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science0,730info:eu-repo/semantics/openAccess2023-11-20T06:15:13Zoai:repositorio.unesp.br:11449/160991Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-20T06:15:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight |
title |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight |
spellingShingle |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight Pasquetti, T. J. Lysine Pigs Response surface Requirements Standardized ileal amino acid digestibility Tryptophan |
title_short |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight |
title_full |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight |
title_fullStr |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight |
title_full_unstemmed |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight |
title_sort |
Simultaneous determination of standardized ileal digestible tryptophan and lysine for barrows from 15 to 30 kg live weight |
author |
Pasquetti, T. J. |
author_facet |
Pasquetti, T. J. Pozza, P. C. Moreira, I. Santos, T. C. Diaz-Huepa, L. M. Castilha, L. D. Perondi, D. [UNESP] Carvalho, P. L. O. Kim, S. W. |
author_role |
author |
author2 |
Pozza, P. C. Moreira, I. Santos, T. C. Diaz-Huepa, L. M. Castilha, L. D. Perondi, D. [UNESP] Carvalho, P. L. O. Kim, S. W. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Mato Grosso do Sul (UEMS) Universidade Estadual de Maringá (UEM) Univ Tecnol Fed Parana Universidade Estadual Paulista (Unesp) Univ Estadual Oeste Parana N Carolina State Univ |
dc.contributor.author.fl_str_mv |
Pasquetti, T. J. Pozza, P. C. Moreira, I. Santos, T. C. Diaz-Huepa, L. M. Castilha, L. D. Perondi, D. [UNESP] Carvalho, P. L. O. Kim, S. W. |
dc.subject.por.fl_str_mv |
Lysine Pigs Response surface Requirements Standardized ileal amino acid digestibility Tryptophan |
topic |
Lysine Pigs Response surface Requirements Standardized ileal amino acid digestibility Tryptophan |
description |
The digestible amino acid (AA) determination of ingredients, or experimental diets, are essential in obtaining the real levels of AA that are being studied, as well as of other indispensable AA, because the lack of a single indispensable AA may limit pig growth performance. Two experiments were conducted to determine the optimum levels and the ideal ratio of standardized ileal digestible (SID) Trp and Lys for barrows from 15 to 30 kg of live weight. The first experiment (Exp. 1) was conducted to determine the SID AA content of the basal diet used in the growth performance study (Exp. 2). Ten crossbred barrows (21.5 +/- 1.6 kg live weight) were used in Exp. 1. Pigs were fed a basal diet and a protein free diet to determine the SID of Trp, Lys, and all other indispensable AA of the basal diet. In Exp. 2, 64 barrows (15.0 +/- 1.6 kg) were allotted to 16 diets in a 4 x 4 factorial arrangement of treatments (1.55, 1.85, 2.15, and 2.45 g SID Trp and 9.72, 11.12, 12.52, and 13.92 g SID Lys/kg) with 4 replications per treatment In both Exp. 1 and 2, each pig was considered as an experimental unit. Growth performance, Longissimus muscle (LM), backfat thickness, and plasma urea nitrogen (PUN) were evaluated. Average daily feed intake and average daily gain increased linearly (P < 0.05) with increasing levels of SID Trp. There was a quadratic effect (P=0.04) of dietary SID Trp and Lys levels on the gain:feed, and the optimal levels were estimated to be 2.1 (0.65 g/Mcal ME) and 11.98 g/kg (3.71 g/Mcal ME), respectively, resulting in a SID Trp:Lys of 17.5:100. A quadratic effect of SID Trp (P=0.021) and Lys (P=0.026) levels on LM was observed, and the optimum levels were 2.08 and 11.36 g/kg diet, respectively, with a Trp:Lys of 183:100. The amount of PUN decreased linearly (P=0.004) as SID Lys increased. For pigs used in our study (between 15 and 30 kg live weight), the SID Trp and SID Lys requirements are 2.10 and 11.98 g/kg, respectively. The corresponding Trp:Lys are 17.5:100. (C) 2015 Elsevier B.V. All rights reserved. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11-01 2018-11-26T16:18:43Z 2018-11-26T16:18:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2015.09.021 Livestock Science. Amsterdam: Elsevier Science Bv, v. 181, p. 114-120, 2015. 1871-1413 http://hdl.handle.net/11449/160991 10.1016/j.livsci.2015.09.021 WOS:000365362800018 WOS:000365362800018.pdf |
url |
http://dx.doi.org/10.1016/j.livsci.2015.09.021 http://hdl.handle.net/11449/160991 |
identifier_str_mv |
Livestock Science. Amsterdam: Elsevier Science Bv, v. 181, p. 114-120, 2015. 1871-1413 10.1016/j.livsci.2015.09.021 WOS:000365362800018 WOS:000365362800018.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science 0,730 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
114-120 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965008604430336 |