Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata

Detalhes bibliográficos
Autor(a) principal: Paulan, Silvana de Cássia [UNESP]
Data de Publicação: 2013
Outros Autores: Gonzáles, Rutilia Marisela Hernándes, Peralta, Laura Adalid, Vicentini-Oliveira, Josy Campanhã [UNESP], Biondi, Germano Francisco [UNESP], Conde, Edda Sciuto, Parkhouse, Robert Michael Evans, Nunes, Caris Maroni [UNESP]
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1984-29612013005000026
http://hdl.handle.net/11449/75020
Resumo: Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method (X + 2DP for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.
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spelling Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginataUsefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovinesELISAExcretion-secretionHP10 antigen assaySanitary inspectionVesicular fluidBovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method (X + 2DP for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Apoio Produção e Saúde Animal Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba, SPInstituto de Investigaciones Biomédicas Universidad Nacional Autónoma de México, Ciudad de Mexico DFEscola de Medicina Veterinária e Zootecnia Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Botucatu, SPInstituto Gulbenkian de Ciências, OeirasDepartamento de Apoio Produção e Saúde Animal Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba, SPEscola de Medicina Veterinária e Zootecnia Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Botucatu, SPCNPq: 482681/2009-8FAPESP: 09/12719-4Universidade Estadual Paulista (Unesp)Universidad Nacional Autónoma de MéxicoInstituto Gulbenkian de CiênciasPaulan, Silvana de Cássia [UNESP]Gonzáles, Rutilia Marisela HernándesPeralta, Laura AdalidVicentini-Oliveira, Josy Campanhã [UNESP]Biondi, Germano Francisco [UNESP]Conde, Edda SciutoParkhouse, Robert Michael EvansNunes, Caris Maroni [UNESP]2014-05-27T11:28:48Z2014-05-27T11:28:48Z2013-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article270-275application/pdfhttp://dx.doi.org/10.1590/S1984-29612013005000026Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 2, p. 270-275, 2013.0103-846Xhttp://hdl.handle.net/11449/7502010.1590/S1984-29612013005000026S1984-29612013005000026WOS:0003225568000152-s2.0-848792524732-s2.0-84879252473.pdf1892359871207408Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Parasitologia Veterináriainfo:eu-repo/semantics/openAccess2024-09-04T19:15:25Zoai:repositorio.unesp.br:11449/75020Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
Usefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovines
title Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
spellingShingle Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
Paulan, Silvana de Cássia [UNESP]
ELISA
Excretion-secretion
HP10 antigen assay
Sanitary inspection
Vesicular fluid
title_short Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
title_full Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
title_fullStr Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
title_full_unstemmed Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
title_sort Utilização de teste sorológico ELISA para a detecção de bovinos naturalmente infectados por Taenia saginata
author Paulan, Silvana de Cássia [UNESP]
author_facet Paulan, Silvana de Cássia [UNESP]
Gonzáles, Rutilia Marisela Hernándes
Peralta, Laura Adalid
Vicentini-Oliveira, Josy Campanhã [UNESP]
Biondi, Germano Francisco [UNESP]
Conde, Edda Sciuto
Parkhouse, Robert Michael Evans
Nunes, Caris Maroni [UNESP]
author_role author
author2 Gonzáles, Rutilia Marisela Hernándes
Peralta, Laura Adalid
Vicentini-Oliveira, Josy Campanhã [UNESP]
Biondi, Germano Francisco [UNESP]
Conde, Edda Sciuto
Parkhouse, Robert Michael Evans
Nunes, Caris Maroni [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidad Nacional Autónoma de México
Instituto Gulbenkian de Ciências
dc.contributor.author.fl_str_mv Paulan, Silvana de Cássia [UNESP]
Gonzáles, Rutilia Marisela Hernándes
Peralta, Laura Adalid
Vicentini-Oliveira, Josy Campanhã [UNESP]
Biondi, Germano Francisco [UNESP]
Conde, Edda Sciuto
Parkhouse, Robert Michael Evans
Nunes, Caris Maroni [UNESP]
dc.subject.por.fl_str_mv ELISA
Excretion-secretion
HP10 antigen assay
Sanitary inspection
Vesicular fluid
topic ELISA
Excretion-secretion
HP10 antigen assay
Sanitary inspection
Vesicular fluid
description Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method (X + 2DP for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-01
2014-05-27T11:28:48Z
2014-05-27T11:28:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612013005000026
Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 2, p. 270-275, 2013.
0103-846X
http://hdl.handle.net/11449/75020
10.1590/S1984-29612013005000026
S1984-29612013005000026
WOS:000322556800015
2-s2.0-84879252473
2-s2.0-84879252473.pdf
1892359871207408
url http://dx.doi.org/10.1590/S1984-29612013005000026
http://hdl.handle.net/11449/75020
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 2, p. 270-275, 2013.
0103-846X
10.1590/S1984-29612013005000026
S1984-29612013005000026
WOS:000322556800015
2-s2.0-84879252473
2-s2.0-84879252473.pdf
1892359871207408
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 270-275
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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