Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S096719941900073X http://hdl.handle.net/11449/209550 |
Resumo: | In vitroembryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use ofl-carnitine duringin vitromaturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM ofl-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented withl-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% inin vitromaturation medium for production of bubaline embryos, and supplementation with 5 mMl-carnitine does not increase embryo production. |
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Repositório Institucional da UNESP |
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Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes?BuffaloesEmbryo developmentFetal bovine serumLipid metabolism modulatorNeutral lipidsIn vitroembryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use ofl-carnitine duringin vitromaturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM ofl-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented withl-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% inin vitromaturation medium for production of bubaline embryos, and supplementation with 5 mMl-carnitine does not increase embryo production.Empresa Brasileira de Pesquisa Agropecuaria - EmbrapaEmbrapa Eastern Amazon, Belem, Para, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Vet Med Dept, Pirassununga, SP, BrazilEmbrapa Dairy Cattle, Juiz De Fora, MG, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuaria - Embrapa: SEG: 02.13.06.020.00.02.001Cambridge Univ PressEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Figueiro, Marivaldo Rodrigues [UNESP]Garcia, Joaquim Mansano [UNESP]Lima, Marina Ragagnin de [UNESP]del Collado, MaiteSaraiva, Naiara Zoccal2021-06-25T12:22:01Z2021-06-25T12:22:01Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article109-115http://dx.doi.org/10.1017/S096719941900073XZygote. New York: Cambridge Univ Press, v. 28, n. 2, p. 109-115, 2020.0967-1994http://hdl.handle.net/11449/20955010.1017/S096719941900073XWOS:000578224800005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygoteinfo:eu-repo/semantics/openAccess2024-06-06T18:09:01Zoai:repositorio.unesp.br:11449/209550Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-06T18:09:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? |
title |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? |
spellingShingle |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? Figueiro, Marivaldo Rodrigues [UNESP] Buffaloes Embryo development Fetal bovine serum Lipid metabolism modulator Neutral lipids |
title_short |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? |
title_full |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? |
title_fullStr |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? |
title_full_unstemmed |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? |
title_sort |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use ofl-carnitine forin vitromaturation of bubaline oocytes? |
author |
Figueiro, Marivaldo Rodrigues [UNESP] |
author_facet |
Figueiro, Marivaldo Rodrigues [UNESP] Garcia, Joaquim Mansano [UNESP] Lima, Marina Ragagnin de [UNESP] del Collado, Maite Saraiva, Naiara Zoccal |
author_role |
author |
author2 |
Garcia, Joaquim Mansano [UNESP] Lima, Marina Ragagnin de [UNESP] del Collado, Maite Saraiva, Naiara Zoccal |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Figueiro, Marivaldo Rodrigues [UNESP] Garcia, Joaquim Mansano [UNESP] Lima, Marina Ragagnin de [UNESP] del Collado, Maite Saraiva, Naiara Zoccal |
dc.subject.por.fl_str_mv |
Buffaloes Embryo development Fetal bovine serum Lipid metabolism modulator Neutral lipids |
topic |
Buffaloes Embryo development Fetal bovine serum Lipid metabolism modulator Neutral lipids |
description |
In vitroembryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use ofl-carnitine duringin vitromaturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM ofl-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented withl-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% inin vitromaturation medium for production of bubaline embryos, and supplementation with 5 mMl-carnitine does not increase embryo production. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-01 2021-06-25T12:22:01Z 2021-06-25T12:22:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S096719941900073X Zygote. New York: Cambridge Univ Press, v. 28, n. 2, p. 109-115, 2020. 0967-1994 http://hdl.handle.net/11449/209550 10.1017/S096719941900073X WOS:000578224800005 |
url |
http://dx.doi.org/10.1017/S096719941900073X http://hdl.handle.net/11449/209550 |
identifier_str_mv |
Zygote. New York: Cambridge Univ Press, v. 28, n. 2, p. 109-115, 2020. 0967-1994 10.1017/S096719941900073X WOS:000578224800005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zygote |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
109-115 |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045212154494976 |