Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2225/vol15-issue5-fulltext-5 http://hdl.handle.net/11449/40609 |
Resumo: | Background: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH were 40 degrees C and 5.0, respectively. The enzyme was fully stable from 40 degrees C to 60 degrees C during 1 hr. The activity was enhanced by Mn2+ (33-39%) and NH4+ (15%). The purified tannase hydrolyzed tannic acid and methyl gallate with Km of 0.76 mM and 0.72 mM, respectively, and Vmax of 0.92 U/mg protein and 0.68 U/mg protein, respectively. The analysis of a partial sequence of the tannase encoding gene showed an open read frame of 567 bp and a sequence of 199 amino acids were predicted. TLC analysis revealed the presence of gallic acid as a tannic acid hydrolysis product. Conclusion: The extracellular tannase produced by A. ochraceus showed distinctive characteristics such as monomeric structure and activation by Mn2+, suggesting a new kind of fungal tannases with biotechnological potential. Further, it was the first time that a partial gene sequence for A. ochraceus tannase was described. |
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Repositório Institucional da UNESP |
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spelling |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceusAspergillus ochraceusgallic acidtannasetannic acidtannin acyl hydrolaseBackground: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH were 40 degrees C and 5.0, respectively. The enzyme was fully stable from 40 degrees C to 60 degrees C during 1 hr. The activity was enhanced by Mn2+ (33-39%) and NH4+ (15%). The purified tannase hydrolyzed tannic acid and methyl gallate with Km of 0.76 mM and 0.72 mM, respectively, and Vmax of 0.92 U/mg protein and 0.68 U/mg protein, respectively. The analysis of a partial sequence of the tannase encoding gene showed an open read frame of 567 bp and a sequence of 199 amino acids were predicted. TLC analysis revealed the presence of gallic acid as a tannic acid hydrolysis product. Conclusion: The extracellular tannase produced by A. ochraceus showed distinctive characteristics such as monomeric structure and activation by Mn2+, suggesting a new kind of fungal tannases with biotechnological potential. Further, it was the first time that a partial gene sequence for A. ochraceus tannase was described.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Inst Quim, UNESP, Araraquara, SP, BrazilUniv São Paulo, Fac Filosofia Ciencias & Letras, Dept Biol, BR-05508 São Paulo, BrazilUniv Estadual Paulista, Inst Quim, UNESP, Araraquara, SP, BrazilUniversidade Católica de ValparaísoUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Goncalves, Heloisa Bressan [UNESP]Riul, Alana Jacomini [UNESP]Quiapim, Andrea CarlaJorge, Joao AtilioSouza Guimaraes, Luis Henrique2014-05-20T15:31:29Z2014-05-20T15:31:29Z2012-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.2225/vol15-issue5-fulltext-5Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 5, p. 12, 2012.0717-3458http://hdl.handle.net/11449/4060910.2225/vol15-issue5-fulltext-5WOS:000314273600008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengElectronic Journal of Biotechnology1.8810,537info:eu-repo/semantics/openAccess2021-10-23T10:41:07Zoai:repositorio.unesp.br:11449/40609Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:48:54.466858Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus |
title |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus |
spellingShingle |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus Goncalves, Heloisa Bressan [UNESP] Aspergillus ochraceus gallic acid tannase tannic acid tannin acyl hydrolase |
title_short |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus |
title_full |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus |
title_fullStr |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus |
title_full_unstemmed |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus |
title_sort |
Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus |
author |
Goncalves, Heloisa Bressan [UNESP] |
author_facet |
Goncalves, Heloisa Bressan [UNESP] Riul, Alana Jacomini [UNESP] Quiapim, Andrea Carla Jorge, Joao Atilio Souza Guimaraes, Luis Henrique |
author_role |
author |
author2 |
Riul, Alana Jacomini [UNESP] Quiapim, Andrea Carla Jorge, Joao Atilio Souza Guimaraes, Luis Henrique |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Goncalves, Heloisa Bressan [UNESP] Riul, Alana Jacomini [UNESP] Quiapim, Andrea Carla Jorge, Joao Atilio Souza Guimaraes, Luis Henrique |
dc.subject.por.fl_str_mv |
Aspergillus ochraceus gallic acid tannase tannic acid tannin acyl hydrolase |
topic |
Aspergillus ochraceus gallic acid tannase tannic acid tannin acyl hydrolase |
description |
Background: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH were 40 degrees C and 5.0, respectively. The enzyme was fully stable from 40 degrees C to 60 degrees C during 1 hr. The activity was enhanced by Mn2+ (33-39%) and NH4+ (15%). The purified tannase hydrolyzed tannic acid and methyl gallate with Km of 0.76 mM and 0.72 mM, respectively, and Vmax of 0.92 U/mg protein and 0.68 U/mg protein, respectively. The analysis of a partial sequence of the tannase encoding gene showed an open read frame of 567 bp and a sequence of 199 amino acids were predicted. TLC analysis revealed the presence of gallic acid as a tannic acid hydrolysis product. Conclusion: The extracellular tannase produced by A. ochraceus showed distinctive characteristics such as monomeric structure and activation by Mn2+, suggesting a new kind of fungal tannases with biotechnological potential. Further, it was the first time that a partial gene sequence for A. ochraceus tannase was described. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-15 2014-05-20T15:31:29Z 2014-05-20T15:31:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2225/vol15-issue5-fulltext-5 Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 5, p. 12, 2012. 0717-3458 http://hdl.handle.net/11449/40609 10.2225/vol15-issue5-fulltext-5 WOS:000314273600008 |
url |
http://dx.doi.org/10.2225/vol15-issue5-fulltext-5 http://hdl.handle.net/11449/40609 |
identifier_str_mv |
Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 5, p. 12, 2012. 0717-3458 10.2225/vol15-issue5-fulltext-5 WOS:000314273600008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Electronic Journal of Biotechnology 1.881 0,537 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Universidade Católica de Valparaíso |
publisher.none.fl_str_mv |
Universidade Católica de Valparaíso |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129464519884800 |