Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus

Detalhes bibliográficos
Autor(a) principal: Goncalves, Heloisa Bressan [UNESP]
Data de Publicação: 2012
Outros Autores: Riul, Alana Jacomini [UNESP], Quiapim, Andrea Carla, Jorge, Joao Atilio, Souza Guimaraes, Luis Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2225/vol15-issue5-fulltext-5
http://hdl.handle.net/11449/40609
Resumo: Background: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH were 40 degrees C and 5.0, respectively. The enzyme was fully stable from 40 degrees C to 60 degrees C during 1 hr. The activity was enhanced by Mn2+ (33-39%) and NH4+ (15%). The purified tannase hydrolyzed tannic acid and methyl gallate with Km of 0.76 mM and 0.72 mM, respectively, and Vmax of 0.92 U/mg protein and 0.68 U/mg protein, respectively. The analysis of a partial sequence of the tannase encoding gene showed an open read frame of 567 bp and a sequence of 199 amino acids were predicted. TLC analysis revealed the presence of gallic acid as a tannic acid hydrolysis product. Conclusion: The extracellular tannase produced by A. ochraceus showed distinctive characteristics such as monomeric structure and activation by Mn2+, suggesting a new kind of fungal tannases with biotechnological potential. Further, it was the first time that a partial gene sequence for A. ochraceus tannase was described.
id UNSP_4eb7e2743abbe5db73bb503973cae42e
oai_identifier_str oai:repositorio.unesp.br:11449/40609
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceusAspergillus ochraceusgallic acidtannasetannic acidtannin acyl hydrolaseBackground: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH were 40 degrees C and 5.0, respectively. The enzyme was fully stable from 40 degrees C to 60 degrees C during 1 hr. The activity was enhanced by Mn2+ (33-39%) and NH4+ (15%). The purified tannase hydrolyzed tannic acid and methyl gallate with Km of 0.76 mM and 0.72 mM, respectively, and Vmax of 0.92 U/mg protein and 0.68 U/mg protein, respectively. The analysis of a partial sequence of the tannase encoding gene showed an open read frame of 567 bp and a sequence of 199 amino acids were predicted. TLC analysis revealed the presence of gallic acid as a tannic acid hydrolysis product. Conclusion: The extracellular tannase produced by A. ochraceus showed distinctive characteristics such as monomeric structure and activation by Mn2+, suggesting a new kind of fungal tannases with biotechnological potential. Further, it was the first time that a partial gene sequence for A. ochraceus tannase was described.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Inst Quim, UNESP, Araraquara, SP, BrazilUniv São Paulo, Fac Filosofia Ciencias & Letras, Dept Biol, BR-05508 São Paulo, BrazilUniv Estadual Paulista, Inst Quim, UNESP, Araraquara, SP, BrazilUniversidade Católica de ValparaísoUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Goncalves, Heloisa Bressan [UNESP]Riul, Alana Jacomini [UNESP]Quiapim, Andrea CarlaJorge, Joao AtilioSouza Guimaraes, Luis Henrique2014-05-20T15:31:29Z2014-05-20T15:31:29Z2012-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.2225/vol15-issue5-fulltext-5Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 5, p. 12, 2012.0717-3458http://hdl.handle.net/11449/4060910.2225/vol15-issue5-fulltext-5WOS:000314273600008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengElectronic Journal of Biotechnology1.8810,537info:eu-repo/semantics/openAccess2021-10-23T10:41:07Zoai:repositorio.unesp.br:11449/40609Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:48:54.466858Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
title Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
spellingShingle Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
Goncalves, Heloisa Bressan [UNESP]
Aspergillus ochraceus
gallic acid
tannase
tannic acid
tannin acyl hydrolase
title_short Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
title_full Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
title_fullStr Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
title_full_unstemmed Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
title_sort Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus
author Goncalves, Heloisa Bressan [UNESP]
author_facet Goncalves, Heloisa Bressan [UNESP]
Riul, Alana Jacomini [UNESP]
Quiapim, Andrea Carla
Jorge, Joao Atilio
Souza Guimaraes, Luis Henrique
author_role author
author2 Riul, Alana Jacomini [UNESP]
Quiapim, Andrea Carla
Jorge, Joao Atilio
Souza Guimaraes, Luis Henrique
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Goncalves, Heloisa Bressan [UNESP]
Riul, Alana Jacomini [UNESP]
Quiapim, Andrea Carla
Jorge, Joao Atilio
Souza Guimaraes, Luis Henrique
dc.subject.por.fl_str_mv Aspergillus ochraceus
gallic acid
tannase
tannic acid
tannin acyl hydrolase
topic Aspergillus ochraceus
gallic acid
tannase
tannic acid
tannin acyl hydrolase
description Background: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH were 40 degrees C and 5.0, respectively. The enzyme was fully stable from 40 degrees C to 60 degrees C during 1 hr. The activity was enhanced by Mn2+ (33-39%) and NH4+ (15%). The purified tannase hydrolyzed tannic acid and methyl gallate with Km of 0.76 mM and 0.72 mM, respectively, and Vmax of 0.92 U/mg protein and 0.68 U/mg protein, respectively. The analysis of a partial sequence of the tannase encoding gene showed an open read frame of 567 bp and a sequence of 199 amino acids were predicted. TLC analysis revealed the presence of gallic acid as a tannic acid hydrolysis product. Conclusion: The extracellular tannase produced by A. ochraceus showed distinctive characteristics such as monomeric structure and activation by Mn2+, suggesting a new kind of fungal tannases with biotechnological potential. Further, it was the first time that a partial gene sequence for A. ochraceus tannase was described.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-15
2014-05-20T15:31:29Z
2014-05-20T15:31:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2225/vol15-issue5-fulltext-5
Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 5, p. 12, 2012.
0717-3458
http://hdl.handle.net/11449/40609
10.2225/vol15-issue5-fulltext-5
WOS:000314273600008
url http://dx.doi.org/10.2225/vol15-issue5-fulltext-5
http://hdl.handle.net/11449/40609
identifier_str_mv Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 5, p. 12, 2012.
0717-3458
10.2225/vol15-issue5-fulltext-5
WOS:000314273600008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Electronic Journal of Biotechnology
1.881
0,537
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Universidade Católica de Valparaíso
publisher.none.fl_str_mv Universidade Católica de Valparaíso
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129464519884800