In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1177/0748233716674398 http://hdl.handle.net/11449/169792 |
Resumo: | A novel composite material has been developed from natural rubber and leather waste, and a corresponding patent has been filed. This new material may be incorporated into textile and footwear products. However, as leather waste contains chromium, the biocompatibility of this new material and its safety for use in humans must be investigated. The aim of the present study was to investigate the presence of chromium in this new material, determine the amount of each form of chromium present (trivalent or hexavalent), and evaluate the potential cytotoxic and genotoxic effects of the novel composite in two cell lines. The cellular viability was quantified using the MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction method and neutral red uptake assay, and genotoxic damage was analyzed using the comet assay. Our findings indicated that the extracts obtained from the composite were severely cytotoxic to both cell lines tested, and additionally highly genotoxic to MRC-5 cells. These biological responses do not appear to be attributable to the presence of chromium, as the trivalent form was predominantly found to be present in the extracts, indicating that hexavalent chromium is not formed during the production of the novel composite. The incorporation of this new material in applications that do not involve direct contact with the human skin is thus indicated, and it is suggested that the chain of production of this material be studied in order to improve its biocompatibility so that it may safely be used in the textile and footwear industries. |
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In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applicationsbiocompatibilitycell lineChromiumleathernatural rubberA novel composite material has been developed from natural rubber and leather waste, and a corresponding patent has been filed. This new material may be incorporated into textile and footwear products. However, as leather waste contains chromium, the biocompatibility of this new material and its safety for use in humans must be investigated. The aim of the present study was to investigate the presence of chromium in this new material, determine the amount of each form of chromium present (trivalent or hexavalent), and evaluate the potential cytotoxic and genotoxic effects of the novel composite in two cell lines. The cellular viability was quantified using the MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction method and neutral red uptake assay, and genotoxic damage was analyzed using the comet assay. Our findings indicated that the extracts obtained from the composite were severely cytotoxic to both cell lines tested, and additionally highly genotoxic to MRC-5 cells. These biological responses do not appear to be attributable to the presence of chromium, as the trivalent form was predominantly found to be present in the extracts, indicating that hexavalent chromium is not formed during the production of the novel composite. The incorporation of this new material in applications that do not involve direct contact with the human skin is thus indicated, and it is suggested that the chain of production of this material be studied in order to improve its biocompatibility so that it may safely be used in the textile and footwear industries.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculty of Science and Technology Department of Physics Chemistry and Biology UNESP, CP 467Faculty of Science and Technology Department of Physics Chemistry and Biology UNESP, CP 467FAPESP: 2013/24884-5Universidade Estadual Paulista (Unesp)Cavalcante, Dalita G.S.M. [UNESP]Gomes, Andressa S. [UNESP]Dos Reis, Elton A.P. [UNESP]Danna, Caroline S. [UNESP]Kerche-Silva, Leandra E. [UNESP]Yoshihara, Eidi [UNESP]Job, Aldo E. [UNESP]2018-12-11T16:47:37Z2018-12-11T16:47:37Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article478-486application/pdfhttp://dx.doi.org/10.1177/0748233716674398Toxicology and Industrial Health, v. 33, n. 6, p. 478-486, 2017.1477-03930748-2337http://hdl.handle.net/11449/16979210.1177/07482337166743982-s2.0-850200717222-s2.0-85020071722.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicology and Industrial Health0,3710,371info:eu-repo/semantics/openAccess2023-11-23T06:12:14Zoai:repositorio.unesp.br:11449/169792Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-23T06:12:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications |
title |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications |
spellingShingle |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications Cavalcante, Dalita G.S.M. [UNESP] biocompatibility cell line Chromium leather natural rubber |
title_short |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications |
title_full |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications |
title_fullStr |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications |
title_full_unstemmed |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications |
title_sort |
In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications |
author |
Cavalcante, Dalita G.S.M. [UNESP] |
author_facet |
Cavalcante, Dalita G.S.M. [UNESP] Gomes, Andressa S. [UNESP] Dos Reis, Elton A.P. [UNESP] Danna, Caroline S. [UNESP] Kerche-Silva, Leandra E. [UNESP] Yoshihara, Eidi [UNESP] Job, Aldo E. [UNESP] |
author_role |
author |
author2 |
Gomes, Andressa S. [UNESP] Dos Reis, Elton A.P. [UNESP] Danna, Caroline S. [UNESP] Kerche-Silva, Leandra E. [UNESP] Yoshihara, Eidi [UNESP] Job, Aldo E. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cavalcante, Dalita G.S.M. [UNESP] Gomes, Andressa S. [UNESP] Dos Reis, Elton A.P. [UNESP] Danna, Caroline S. [UNESP] Kerche-Silva, Leandra E. [UNESP] Yoshihara, Eidi [UNESP] Job, Aldo E. [UNESP] |
dc.subject.por.fl_str_mv |
biocompatibility cell line Chromium leather natural rubber |
topic |
biocompatibility cell line Chromium leather natural rubber |
description |
A novel composite material has been developed from natural rubber and leather waste, and a corresponding patent has been filed. This new material may be incorporated into textile and footwear products. However, as leather waste contains chromium, the biocompatibility of this new material and its safety for use in humans must be investigated. The aim of the present study was to investigate the presence of chromium in this new material, determine the amount of each form of chromium present (trivalent or hexavalent), and evaluate the potential cytotoxic and genotoxic effects of the novel composite in two cell lines. The cellular viability was quantified using the MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction method and neutral red uptake assay, and genotoxic damage was analyzed using the comet assay. Our findings indicated that the extracts obtained from the composite were severely cytotoxic to both cell lines tested, and additionally highly genotoxic to MRC-5 cells. These biological responses do not appear to be attributable to the presence of chromium, as the trivalent form was predominantly found to be present in the extracts, indicating that hexavalent chromium is not formed during the production of the novel composite. The incorporation of this new material in applications that do not involve direct contact with the human skin is thus indicated, and it is suggested that the chain of production of this material be studied in order to improve its biocompatibility so that it may safely be used in the textile and footwear industries. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-12-11T16:47:37Z 2018-12-11T16:47:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1177/0748233716674398 Toxicology and Industrial Health, v. 33, n. 6, p. 478-486, 2017. 1477-0393 0748-2337 http://hdl.handle.net/11449/169792 10.1177/0748233716674398 2-s2.0-85020071722 2-s2.0-85020071722.pdf |
url |
http://dx.doi.org/10.1177/0748233716674398 http://hdl.handle.net/11449/169792 |
identifier_str_mv |
Toxicology and Industrial Health, v. 33, n. 6, p. 478-486, 2017. 1477-0393 0748-2337 10.1177/0748233716674398 2-s2.0-85020071722 2-s2.0-85020071722.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology and Industrial Health 0,371 0,371 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
478-486 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803649793217527808 |