Production of the Quinone-Methide Triterpene Maytenin by In Vitro Adventitious Roots of Peritassa campestris (Cambess.) ACSm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MS/MS

• Autor(a) principal: Paz, Tiago Antunes [UNESP]
• Data de Publicação: 2013
• Outros Autores: Santos, Vania A. F. F. M. dos [UNESP], Inacio, Marielle Cascaes [UNESP], Pina, Edieidia Souza, Soares Pereira, Ana Maria, Furlan, Maysa [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1155/2013/485837; http://hdl.handle.net/11449/113195

Establishment of adventitious root cultures of Peritassa campestris (Celastraceae) was achieved from seed cotyledons cultured in semisolid Woody Plant Medium (WPM) supplemented with 2% sucrose, 0.01% PVP, and 4.0mg L-1 IBA. Culture period on accumulation of biomass and quinone-methide triterpene maytenin in adventitious root were investigated. The accumulation of maytenin in these roots was compared with its accumulation in the roots of seedlings grown in a greenhouse (one year old). A rapid detection and identification of maytenin by direct injection into an atmospheric-pressure chemical ionization ion trap tandem mass spectrometer (APCI-IT-MS/MS) were performed without prior chromatographic separation. In vitro, the greatest accumulation of biomass occurred within 60 days of culture. The highest level of maytenin-972.11 mu g.g(-1) dry weight-was detected at seven days of cultivation; this value was 5.55-fold higher than that found in the roots of seedlings grown in a greenhouse.