EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4132/jptm.2022.04.22 http://hdl.handle.net/11449/241570 |
Resumo: | Background: Despite the advances in glioblastoma (GBM) treatment, the average life span of patients is 14 months. Therefore, it is urgent to identity biomarkers of prognosis, treatment response, or development of novel treatment strategies. We previously described the association of high epidermal growth factor-like domain multiple 7 (EGFL7) expression and unfavorable outcome of pilocytic astrocytoma patients. The present study aims to analyze the prognostic potential of EGFL7 in GBM isocitrate dehydrogenase (IDH)-wildtype, using immunohistochemistry and in silico approaches. Methods: Spearman's correlation analysis of The Cancer Genome Atlas RNA sequencing data was performed. The genes strongly correlated to EGFL7 expression were submitted to enrichment gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Additionally, EGFL7 expression was associated with patient overall survival. The expression of EGFL7 was analyzed through immunohistochemistry in 74 GBM IDH-wildtype patients' samples, and was associated with clinicopathological data and overall survival. Results: In silico analysis found 78 genes strongly correlated to EGFL7 expression. These genes were enriched in 40 biological processes and eight KEGG pathways, including angiogenesis/vasculogenesis, cell adhesion, and phosphoinositide 3-kinase-Akt, Notch, and Rap1 signaling pathways. The immunostaining showed high EGFL7 expression in 39 cases (52.7%). High immunolabelling was significantly associated with low Karnofsky Performance Status and poor overall survival. Cox analysis showed that GBMs IDH-wildtype with high EGFL7 expression presented a higher risk of death compared to low expression (hazard ratio, 1.645; 95% confidence interval, 1.021 to 2.650; p = .041). Conclusions: This study gives insights regarding the genes that are correlated with EGFL7, as well as biological processes and signaling pathways, which should be further investigated in order to elucidate their role in glioblastoma biology. |
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EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcomeEGFL7Glioblastoma IDH-wildtypeNotch pathwayPI3K-Akt pathwayRap1 pathwayBackground: Despite the advances in glioblastoma (GBM) treatment, the average life span of patients is 14 months. Therefore, it is urgent to identity biomarkers of prognosis, treatment response, or development of novel treatment strategies. We previously described the association of high epidermal growth factor-like domain multiple 7 (EGFL7) expression and unfavorable outcome of pilocytic astrocytoma patients. The present study aims to analyze the prognostic potential of EGFL7 in GBM isocitrate dehydrogenase (IDH)-wildtype, using immunohistochemistry and in silico approaches. Methods: Spearman's correlation analysis of The Cancer Genome Atlas RNA sequencing data was performed. The genes strongly correlated to EGFL7 expression were submitted to enrichment gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Additionally, EGFL7 expression was associated with patient overall survival. The expression of EGFL7 was analyzed through immunohistochemistry in 74 GBM IDH-wildtype patients' samples, and was associated with clinicopathological data and overall survival. Results: In silico analysis found 78 genes strongly correlated to EGFL7 expression. These genes were enriched in 40 biological processes and eight KEGG pathways, including angiogenesis/vasculogenesis, cell adhesion, and phosphoinositide 3-kinase-Akt, Notch, and Rap1 signaling pathways. The immunostaining showed high EGFL7 expression in 39 cases (52.7%). High immunolabelling was significantly associated with low Karnofsky Performance Status and poor overall survival. Cox analysis showed that GBMs IDH-wildtype with high EGFL7 expression presented a higher risk of death compared to low expression (hazard ratio, 1.645; 95% confidence interval, 1.021 to 2.650; p = .041). Conclusions: This study gives insights regarding the genes that are correlated with EGFL7, as well as biological processes and signaling pathways, which should be further investigated in order to elucidate their role in glioblastoma biology.Molecular Oncology Research Center Barretos Cancer Hospital, BarretosBarretos School of Health Sciences Dr. Paulo Prata - FACISB, BarretosOhio State University Department of Radiation OncologyDepartment of Pathology School of Medicine of Ribeirao Preto University of Sao Paulo, Ribeirao PretoUNESP - Univ. Estadual Paulista School of Medicine Department of Pathology, BotucatuDepartment of Neurosurgery Barretos Cancer Hospital, BarretosDepartment of Pathology Barretos Cancer Hospital, BarretosLife and Health Sciences Research Institute (ICVS) School of Medicine University of MinhoICVS/3B's - PT Government Associate Laboratory, GuimaraesUNESP - Univ. Estadual Paulista School of Medicine Department of Pathology, BotucatuBarretos Cancer HospitalDr. Paulo Prata - FACISBOhio State UniversityUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)University of MinhoICVS/3B's - PT Government Associate LaboratoryDa Costa, Bruno Henrique BressanBecker, Aline PaixaoNeder, LucianoGoncalves, Paola Gyuliane [UNESP]De Oliveira, Cristiane [UNESP]Polverini, Allan DiasClara, Carlos AfonsoTeixeira, Gustavo RamosReis, Rui ManuelBidinotto, Lucas Tadeu [UNESP]2023-03-01T21:10:46Z2023-03-01T21:10:46Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article205-211http://dx.doi.org/10.4132/jptm.2022.04.22Journal of Pathology and Translational Medicine, v. 56, n. 4, p. 205-211, 2022.2383-78452383-7837http://hdl.handle.net/11449/24157010.4132/jptm.2022.04.222-s2.0-85136908523Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Pathology and Translational Medicineinfo:eu-repo/semantics/openAccess2023-03-01T21:10:47Zoai:repositorio.unesp.br:11449/241570Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T21:10:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome |
title |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome |
spellingShingle |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome Da Costa, Bruno Henrique Bressan EGFL7 Glioblastoma IDH-wildtype Notch pathway PI3K-Akt pathway Rap1 pathway |
title_short |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome |
title_full |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome |
title_fullStr |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome |
title_full_unstemmed |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome |
title_sort |
EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome |
author |
Da Costa, Bruno Henrique Bressan |
author_facet |
Da Costa, Bruno Henrique Bressan Becker, Aline Paixao Neder, Luciano Goncalves, Paola Gyuliane [UNESP] De Oliveira, Cristiane [UNESP] Polverini, Allan Dias Clara, Carlos Afonso Teixeira, Gustavo Ramos Reis, Rui Manuel Bidinotto, Lucas Tadeu [UNESP] |
author_role |
author |
author2 |
Becker, Aline Paixao Neder, Luciano Goncalves, Paola Gyuliane [UNESP] De Oliveira, Cristiane [UNESP] Polverini, Allan Dias Clara, Carlos Afonso Teixeira, Gustavo Ramos Reis, Rui Manuel Bidinotto, Lucas Tadeu [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Barretos Cancer Hospital Dr. Paulo Prata - FACISB Ohio State University Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) University of Minho ICVS/3B's - PT Government Associate Laboratory |
dc.contributor.author.fl_str_mv |
Da Costa, Bruno Henrique Bressan Becker, Aline Paixao Neder, Luciano Goncalves, Paola Gyuliane [UNESP] De Oliveira, Cristiane [UNESP] Polverini, Allan Dias Clara, Carlos Afonso Teixeira, Gustavo Ramos Reis, Rui Manuel Bidinotto, Lucas Tadeu [UNESP] |
dc.subject.por.fl_str_mv |
EGFL7 Glioblastoma IDH-wildtype Notch pathway PI3K-Akt pathway Rap1 pathway |
topic |
EGFL7 Glioblastoma IDH-wildtype Notch pathway PI3K-Akt pathway Rap1 pathway |
description |
Background: Despite the advances in glioblastoma (GBM) treatment, the average life span of patients is 14 months. Therefore, it is urgent to identity biomarkers of prognosis, treatment response, or development of novel treatment strategies. We previously described the association of high epidermal growth factor-like domain multiple 7 (EGFL7) expression and unfavorable outcome of pilocytic astrocytoma patients. The present study aims to analyze the prognostic potential of EGFL7 in GBM isocitrate dehydrogenase (IDH)-wildtype, using immunohistochemistry and in silico approaches. Methods: Spearman's correlation analysis of The Cancer Genome Atlas RNA sequencing data was performed. The genes strongly correlated to EGFL7 expression were submitted to enrichment gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Additionally, EGFL7 expression was associated with patient overall survival. The expression of EGFL7 was analyzed through immunohistochemistry in 74 GBM IDH-wildtype patients' samples, and was associated with clinicopathological data and overall survival. Results: In silico analysis found 78 genes strongly correlated to EGFL7 expression. These genes were enriched in 40 biological processes and eight KEGG pathways, including angiogenesis/vasculogenesis, cell adhesion, and phosphoinositide 3-kinase-Akt, Notch, and Rap1 signaling pathways. The immunostaining showed high EGFL7 expression in 39 cases (52.7%). High immunolabelling was significantly associated with low Karnofsky Performance Status and poor overall survival. Cox analysis showed that GBMs IDH-wildtype with high EGFL7 expression presented a higher risk of death compared to low expression (hazard ratio, 1.645; 95% confidence interval, 1.021 to 2.650; p = .041). Conclusions: This study gives insights regarding the genes that are correlated with EGFL7, as well as biological processes and signaling pathways, which should be further investigated in order to elucidate their role in glioblastoma biology. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-01T21:10:46Z 2023-03-01T21:10:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4132/jptm.2022.04.22 Journal of Pathology and Translational Medicine, v. 56, n. 4, p. 205-211, 2022. 2383-7845 2383-7837 http://hdl.handle.net/11449/241570 10.4132/jptm.2022.04.22 2-s2.0-85136908523 |
url |
http://dx.doi.org/10.4132/jptm.2022.04.22 http://hdl.handle.net/11449/241570 |
identifier_str_mv |
Journal of Pathology and Translational Medicine, v. 56, n. 4, p. 205-211, 2022. 2383-7845 2383-7837 10.4132/jptm.2022.04.22 2-s2.0-85136908523 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Pathology and Translational Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
205-211 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964605028499456 |