Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| DOI: | 10.1016/j.mcp.2018.09.001 |
| Texto Completo: | http://dx.doi.org/10.1016/j.mcp.2018.09.001 http://hdl.handle.net/11449/186438 |
Resumo: | This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healthy animals, respectively, evidencing a trend toward different expressions of this gene in the studied groups. Our finding suggests that LPO and CSF3 genes are an important defense mechanism against mastitis in dairy buffaloes, and may be putative genes for selecting healthier animals in buffalo breeding programs. |
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Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitisCytokineGene expressionImmunityMammary glandPeroxidaseThis study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healthy animals, respectively, evidencing a trend toward different expressions of this gene in the studied groups. Our finding suggests that LPO and CSF3 genes are an important defense mechanism against mastitis in dairy buffaloes, and may be putative genes for selecting healthier animals in buffalo breeding programs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Sci, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Sci, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2014/25309-7Elsevier B.V.Universidade Estadual Paulista (Unesp)Silva Stella, Aline Aparecida [UNESP]Simielli Fonseca, Larissa Fernanda [UNESP]Jovino Gimenez, Daniele Fernanda [UNESP]Tanamati, Fernanda [UNESP]Nascimento, Andre Vieira do [UNESP]Cardoso, Diercles Francisco [UNESP]Tonhati, Humberto [UNESP]2019-10-04T23:10:43Z2019-10-04T23:10:43Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article39-42http://dx.doi.org/10.1016/j.mcp.2018.09.001Molecular And Cellular Probes. London: Academic Press Ltd- Elsevier Science Ltd, v. 41, p. 39-42, 2018.0890-8508http://hdl.handle.net/11449/18643810.1016/j.mcp.2018.09.001WOS:000447224700007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular And Cellular Probesinfo:eu-repo/semantics/openAccess2024-06-07T18:41:31Zoai:repositorio.unesp.br:11449/186438Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:07:14.505718Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis |
| title |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis |
| spellingShingle |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis Silva Stella, Aline Aparecida [UNESP] Cytokine Gene expression Immunity Mammary gland Peroxidase Silva Stella, Aline Aparecida [UNESP] Cytokine Gene expression Immunity Mammary gland Peroxidase |
| title_short |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis |
| title_full |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis |
| title_fullStr |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis |
| title_full_unstemmed |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis |
| title_sort |
Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis |
| author |
Silva Stella, Aline Aparecida [UNESP] |
| author_facet |
Silva Stella, Aline Aparecida [UNESP] Silva Stella, Aline Aparecida [UNESP] Simielli Fonseca, Larissa Fernanda [UNESP] Jovino Gimenez, Daniele Fernanda [UNESP] Tanamati, Fernanda [UNESP] Nascimento, Andre Vieira do [UNESP] Cardoso, Diercles Francisco [UNESP] Tonhati, Humberto [UNESP] Simielli Fonseca, Larissa Fernanda [UNESP] Jovino Gimenez, Daniele Fernanda [UNESP] Tanamati, Fernanda [UNESP] Nascimento, Andre Vieira do [UNESP] Cardoso, Diercles Francisco [UNESP] Tonhati, Humberto [UNESP] |
| author_role |
author |
| author2 |
Simielli Fonseca, Larissa Fernanda [UNESP] Jovino Gimenez, Daniele Fernanda [UNESP] Tanamati, Fernanda [UNESP] Nascimento, Andre Vieira do [UNESP] Cardoso, Diercles Francisco [UNESP] Tonhati, Humberto [UNESP] |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
| dc.contributor.author.fl_str_mv |
Silva Stella, Aline Aparecida [UNESP] Simielli Fonseca, Larissa Fernanda [UNESP] Jovino Gimenez, Daniele Fernanda [UNESP] Tanamati, Fernanda [UNESP] Nascimento, Andre Vieira do [UNESP] Cardoso, Diercles Francisco [UNESP] Tonhati, Humberto [UNESP] |
| dc.subject.por.fl_str_mv |
Cytokine Gene expression Immunity Mammary gland Peroxidase |
| topic |
Cytokine Gene expression Immunity Mammary gland Peroxidase |
| description |
This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healthy animals, respectively, evidencing a trend toward different expressions of this gene in the studied groups. Our finding suggests that LPO and CSF3 genes are an important defense mechanism against mastitis in dairy buffaloes, and may be putative genes for selecting healthier animals in buffalo breeding programs. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-10-01 2019-10-04T23:10:43Z 2019-10-04T23:10:43Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mcp.2018.09.001 Molecular And Cellular Probes. London: Academic Press Ltd- Elsevier Science Ltd, v. 41, p. 39-42, 2018. 0890-8508 http://hdl.handle.net/11449/186438 10.1016/j.mcp.2018.09.001 WOS:000447224700007 |
| url |
http://dx.doi.org/10.1016/j.mcp.2018.09.001 http://hdl.handle.net/11449/186438 |
| identifier_str_mv |
Molecular And Cellular Probes. London: Academic Press Ltd- Elsevier Science Ltd, v. 41, p. 39-42, 2018. 0890-8508 10.1016/j.mcp.2018.09.001 WOS:000447224700007 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Molecular And Cellular Probes |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
39-42 |
| dc.publisher.none.fl_str_mv |
Elsevier B.V. |
| publisher.none.fl_str_mv |
Elsevier B.V. |
| dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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|
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1822182325567356928 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.mcp.2018.09.001 |