Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis

Detalhes bibliográficos
Autor(a) principal: Silva Stella, Aline Aparecida [UNESP]
Data de Publicação: 2018
Outros Autores: Simielli Fonseca, Larissa Fernanda [UNESP], Jovino Gimenez, Daniele Fernanda [UNESP], Tanamati, Fernanda [UNESP], Nascimento, Andre Vieira do [UNESP], Cardoso, Diercles Francisco [UNESP], Tonhati, Humberto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.mcp.2018.09.001
http://hdl.handle.net/11449/186438
Resumo: This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healthy animals, respectively, evidencing a trend toward different expressions of this gene in the studied groups. Our finding suggests that LPO and CSF3 genes are an important defense mechanism against mastitis in dairy buffaloes, and may be putative genes for selecting healthier animals in buffalo breeding programs.
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spelling Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitisCytokineGene expressionImmunityMammary glandPeroxidaseThis study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healthy animals, respectively, evidencing a trend toward different expressions of this gene in the studied groups. Our finding suggests that LPO and CSF3 genes are an important defense mechanism against mastitis in dairy buffaloes, and may be putative genes for selecting healthier animals in buffalo breeding programs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Sci, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Sci, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2014/25309-7Elsevier B.V.Universidade Estadual Paulista (Unesp)Silva Stella, Aline Aparecida [UNESP]Simielli Fonseca, Larissa Fernanda [UNESP]Jovino Gimenez, Daniele Fernanda [UNESP]Tanamati, Fernanda [UNESP]Nascimento, Andre Vieira do [UNESP]Cardoso, Diercles Francisco [UNESP]Tonhati, Humberto [UNESP]2019-10-04T23:10:43Z2019-10-04T23:10:43Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article39-42http://dx.doi.org/10.1016/j.mcp.2018.09.001Molecular And Cellular Probes. London: Academic Press Ltd- Elsevier Science Ltd, v. 41, p. 39-42, 2018.0890-8508http://hdl.handle.net/11449/18643810.1016/j.mcp.2018.09.001WOS:000447224700007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular And Cellular Probesinfo:eu-repo/semantics/openAccess2021-10-23T18:56:51Zoai:repositorio.unesp.br:11449/186438Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T18:56:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
title Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
spellingShingle Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
Silva Stella, Aline Aparecida [UNESP]
Cytokine
Gene expression
Immunity
Mammary gland
Peroxidase
title_short Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
title_full Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
title_fullStr Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
title_full_unstemmed Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
title_sort Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
author Silva Stella, Aline Aparecida [UNESP]
author_facet Silva Stella, Aline Aparecida [UNESP]
Simielli Fonseca, Larissa Fernanda [UNESP]
Jovino Gimenez, Daniele Fernanda [UNESP]
Tanamati, Fernanda [UNESP]
Nascimento, Andre Vieira do [UNESP]
Cardoso, Diercles Francisco [UNESP]
Tonhati, Humberto [UNESP]
author_role author
author2 Simielli Fonseca, Larissa Fernanda [UNESP]
Jovino Gimenez, Daniele Fernanda [UNESP]
Tanamati, Fernanda [UNESP]
Nascimento, Andre Vieira do [UNESP]
Cardoso, Diercles Francisco [UNESP]
Tonhati, Humberto [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva Stella, Aline Aparecida [UNESP]
Simielli Fonseca, Larissa Fernanda [UNESP]
Jovino Gimenez, Daniele Fernanda [UNESP]
Tanamati, Fernanda [UNESP]
Nascimento, Andre Vieira do [UNESP]
Cardoso, Diercles Francisco [UNESP]
Tonhati, Humberto [UNESP]
dc.subject.por.fl_str_mv Cytokine
Gene expression
Immunity
Mammary gland
Peroxidase
topic Cytokine
Gene expression
Immunity
Mammary gland
Peroxidase
description This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healthy animals, respectively, evidencing a trend toward different expressions of this gene in the studied groups. Our finding suggests that LPO and CSF3 genes are an important defense mechanism against mastitis in dairy buffaloes, and may be putative genes for selecting healthier animals in buffalo breeding programs.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-01
2019-10-04T23:10:43Z
2019-10-04T23:10:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.mcp.2018.09.001
Molecular And Cellular Probes. London: Academic Press Ltd- Elsevier Science Ltd, v. 41, p. 39-42, 2018.
0890-8508
http://hdl.handle.net/11449/186438
10.1016/j.mcp.2018.09.001
WOS:000447224700007
url http://dx.doi.org/10.1016/j.mcp.2018.09.001
http://hdl.handle.net/11449/186438
identifier_str_mv Molecular And Cellular Probes. London: Academic Press Ltd- Elsevier Science Ltd, v. 41, p. 39-42, 2018.
0890-8508
10.1016/j.mcp.2018.09.001
WOS:000447224700007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular And Cellular Probes
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 39-42
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964992209944576

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