Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-021-16352-6 http://hdl.handle.net/11449/222450 |
Resumo: | The insecticide fipronil and the herbicide 2,4-D are the most applied pesticides in sugarcane crops leading to aquatic contamination. The whole-body bioconcentration of fipronil and 2,4-D, single and in mixture, was evaluated in Danio rerio after 96-h exposure. The activities of catalase (CAT) and glutathione S-transferase(GST) in whole body and in the gills and the acetylcholinesterase (AChE) in muscle were determined. The gill histopathology and the morphology of the pavement (PVC) and the mitochondria-rich(MRC) cells at gill surface were analyzed. Bioconcentration occurred after exposure to fipronil (2.69 L kg−1) and 2,4-D (1.73 L kg−1) single and in mixture of fipronil (3.10 L kg−1) and 2,4-D (1.27 L kg−1). Whole-body CAT activity was unchanged, and its activity decreased in the gills after exposure to fipronil and increased after exposure to 2,4-D and mixture. GST and AChE increased after single exposure to each pesticide and mixture of both. Fish exposed to mixture increased the MRC fractional area (MRCFA) which suggested possible ionic regulation disturbance and reduced the microridge of the PVC surface. Synergistic interactions occurred in the CAT activity and MRCFA after exposure to mixture of pesticides. The results indicate that the recommended application dose of fipronil and 2,4-D, single or in mixture, for sugarcane crops affects this fish species altering its homeostasis. |
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Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio.AcetylcholinesteraseBioconcentrationCatalaseGlutathione S-transferaseHistopathologyMitochondria-rich cellsThe insecticide fipronil and the herbicide 2,4-D are the most applied pesticides in sugarcane crops leading to aquatic contamination. The whole-body bioconcentration of fipronil and 2,4-D, single and in mixture, was evaluated in Danio rerio after 96-h exposure. The activities of catalase (CAT) and glutathione S-transferase(GST) in whole body and in the gills and the acetylcholinesterase (AChE) in muscle were determined. The gill histopathology and the morphology of the pavement (PVC) and the mitochondria-rich(MRC) cells at gill surface were analyzed. Bioconcentration occurred after exposure to fipronil (2.69 L kg−1) and 2,4-D (1.73 L kg−1) single and in mixture of fipronil (3.10 L kg−1) and 2,4-D (1.27 L kg−1). Whole-body CAT activity was unchanged, and its activity decreased in the gills after exposure to fipronil and increased after exposure to 2,4-D and mixture. GST and AChE increased after single exposure to each pesticide and mixture of both. Fish exposed to mixture increased the MRC fractional area (MRCFA) which suggested possible ionic regulation disturbance and reduced the microridge of the PVC surface. Synergistic interactions occurred in the CAT activity and MRCFA after exposure to mixture of pesticides. The results indicate that the recommended application dose of fipronil and 2,4-D, single or in mixture, for sugarcane crops affects this fish species altering its homeostasis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Programa de Pós-graduação em Ecologia e Recursos Naturais Universidade Federal de São Carlos, Rodovia Washington Luiz, km 235, São PauloDepartamento de Ciências Fisiológicas Universidade Federal de São Carlos (UFSCar), Rodovia Washington Luiz, km 235, SPPrograma de Pós-graduação em Ciências da Engenharia Ambiental Escola de Engenharia de São Carlos (NEEA/CRHEA/SHS) Universidade de São Paulo, Av. Trabalhador São-Carlense, 400, SPPrograma Interinstitucional de Pós-graduação em Ciências Fisiológicas Universidade Federal de São Carlos/Universidade Estadual de São Paulo, Rodovia Washington Luiz, km 235, São PauloFaculdade de Ciências Químicas Universidade Nacional de CórdobaInstituto de Química Universidade de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, SPInstituto de Ciências e Tecnologia de Alimentos de Córdoba CONICETPrograma Interinstitucional de Pós-graduação em Ciências Fisiológicas Universidade Federal de São Carlos/Universidade Estadual de São Paulo, Rodovia Washington Luiz, km 235, São PauloFAPESP: 2015/18790-3CNPq: 306818/2020-5CAPES: Finance code 001Universidade Federal de São Carlos (UFSCar)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Nacional de CórdobaUniversidade Estadual de Campinas (UNICAMP)CONICETViana, Natália Prudêncioda Silva, Laís Conceição MenezesPortruneli, NatáliaSoares, Michelly Pereira [UNESP]Cardoso, Israel Luz [UNESP]Bonansea, Rocío InésGoulart, Bianca VelosoMontagner, Cassiana CarolinaEspíndola, Evaldo Luiz GaetaWunderlin, Daniel AlbertoFernandes, Marisa Narciso2022-04-28T19:44:46Z2022-04-28T19:44:46Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11685-11698http://dx.doi.org/10.1007/s11356-021-16352-6Environmental Science and Pollution Research, v. 29, n. 8, p. 11685-11698, 2022.1614-74990944-1344http://hdl.handle.net/11449/22245010.1007/s11356-021-16352-62-s2.0-85115204206Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccess2022-04-28T19:44:46Zoai:repositorio.unesp.br:11449/222450Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:54:58.849649Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. |
title |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. |
spellingShingle |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. Viana, Natália Prudêncio Acetylcholinesterase Bioconcentration Catalase Glutathione S-transferase Histopathology Mitochondria-rich cells |
title_short |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. |
title_full |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. |
title_fullStr |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. |
title_full_unstemmed |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. |
title_sort |
Bioconcentration and toxicological impacts of fipronil and 2,4-D commercial formulations (single and in mixture) in the tropical fish, Danio rerio. |
author |
Viana, Natália Prudêncio |
author_facet |
Viana, Natália Prudêncio da Silva, Laís Conceição Menezes Portruneli, Natália Soares, Michelly Pereira [UNESP] Cardoso, Israel Luz [UNESP] Bonansea, Rocío Inés Goulart, Bianca Veloso Montagner, Cassiana Carolina Espíndola, Evaldo Luiz Gaeta Wunderlin, Daniel Alberto Fernandes, Marisa Narciso |
author_role |
author |
author2 |
da Silva, Laís Conceição Menezes Portruneli, Natália Soares, Michelly Pereira [UNESP] Cardoso, Israel Luz [UNESP] Bonansea, Rocío Inés Goulart, Bianca Veloso Montagner, Cassiana Carolina Espíndola, Evaldo Luiz Gaeta Wunderlin, Daniel Alberto Fernandes, Marisa Narciso |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Universidade Nacional de Córdoba Universidade Estadual de Campinas (UNICAMP) CONICET |
dc.contributor.author.fl_str_mv |
Viana, Natália Prudêncio da Silva, Laís Conceição Menezes Portruneli, Natália Soares, Michelly Pereira [UNESP] Cardoso, Israel Luz [UNESP] Bonansea, Rocío Inés Goulart, Bianca Veloso Montagner, Cassiana Carolina Espíndola, Evaldo Luiz Gaeta Wunderlin, Daniel Alberto Fernandes, Marisa Narciso |
dc.subject.por.fl_str_mv |
Acetylcholinesterase Bioconcentration Catalase Glutathione S-transferase Histopathology Mitochondria-rich cells |
topic |
Acetylcholinesterase Bioconcentration Catalase Glutathione S-transferase Histopathology Mitochondria-rich cells |
description |
The insecticide fipronil and the herbicide 2,4-D are the most applied pesticides in sugarcane crops leading to aquatic contamination. The whole-body bioconcentration of fipronil and 2,4-D, single and in mixture, was evaluated in Danio rerio after 96-h exposure. The activities of catalase (CAT) and glutathione S-transferase(GST) in whole body and in the gills and the acetylcholinesterase (AChE) in muscle were determined. The gill histopathology and the morphology of the pavement (PVC) and the mitochondria-rich(MRC) cells at gill surface were analyzed. Bioconcentration occurred after exposure to fipronil (2.69 L kg−1) and 2,4-D (1.73 L kg−1) single and in mixture of fipronil (3.10 L kg−1) and 2,4-D (1.27 L kg−1). Whole-body CAT activity was unchanged, and its activity decreased in the gills after exposure to fipronil and increased after exposure to 2,4-D and mixture. GST and AChE increased after single exposure to each pesticide and mixture of both. Fish exposed to mixture increased the MRC fractional area (MRCFA) which suggested possible ionic regulation disturbance and reduced the microridge of the PVC surface. Synergistic interactions occurred in the CAT activity and MRCFA after exposure to mixture of pesticides. The results indicate that the recommended application dose of fipronil and 2,4-D, single or in mixture, for sugarcane crops affects this fish species altering its homeostasis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:44:46Z 2022-04-28T19:44:46Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-021-16352-6 Environmental Science and Pollution Research, v. 29, n. 8, p. 11685-11698, 2022. 1614-7499 0944-1344 http://hdl.handle.net/11449/222450 10.1007/s11356-021-16352-6 2-s2.0-85115204206 |
url |
http://dx.doi.org/10.1007/s11356-021-16352-6 http://hdl.handle.net/11449/222450 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 29, n. 8, p. 11685-11698, 2022. 1614-7499 0944-1344 10.1007/s11356-021-16352-6 2-s2.0-85115204206 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11685-11698 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129138576326656 |