Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0067 http://hdl.handle.net/11449/196941 |
Resumo: | Background: Extracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (Bubalus bubalis) sera infected with Theileria spp. Methods: Infected animals were identified through optical microscopy and PCR. EVs were isolated from buffalo sera by size-exclusion chromatography and characterized using western blotting analysis, nanoparticle tracking analysis and transmission electron microscopy. Subsequently, the proteins from isolated vesicles were characterized by mass spectrometry. Results: EVs from buffalo sera have shown sizes in the 124-140 nm range and 306 proteins were characterized. The protein-protein interaction analysis has evidenced biological processes and molecular function associated with signal transduction, binding, regulation of metabolic processes, transport, catalytic activity and response to acute stress. Five proteins have been shown to be differentially expressed between the control group and that infected with Theileria spp., all acting in the oxidative stress pathway. Conclusions: EVs from buffaloes infected with Theileria spp. were successfully isolated and characterized. This is an advance in the knowledge of host-parasite relationship that contributes to the understanding of host immune response and theileriosis evasion mechanisms. These findings may pave the way for searching new EVs candidate-markers for a better production of safe biological products derived from buffaloes. |
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Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloesExtracellular vesiclesTheileria spp.Protozoan parasitesNanoparticle tracking analysisProteomic analysisBackground: Extracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (Bubalus bubalis) sera infected with Theileria spp. Methods: Infected animals were identified through optical microscopy and PCR. EVs were isolated from buffalo sera by size-exclusion chromatography and characterized using western blotting analysis, nanoparticle tracking analysis and transmission electron microscopy. Subsequently, the proteins from isolated vesicles were characterized by mass spectrometry. Results: EVs from buffalo sera have shown sizes in the 124-140 nm range and 306 proteins were characterized. The protein-protein interaction analysis has evidenced biological processes and molecular function associated with signal transduction, binding, regulation of metabolic processes, transport, catalytic activity and response to acute stress. Five proteins have been shown to be differentially expressed between the control group and that infected with Theileria spp., all acting in the oxidative stress pathway. Conclusions: EVs from buffaloes infected with Theileria spp. were successfully isolated and characterized. This is an advance in the knowledge of host-parasite relationship that contributes to the understanding of host immune response and theileriosis evasion mechanisms. These findings may pave the way for searching new EVs candidate-markers for a better production of safe biological products derived from buffaloes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ UNESP, Botucatu Med Sch FMB, Grad Program Trop Dis, Botucatu, SP, BrazilFed Univ Sao Carlos UFSCar, Dept Physiol Sci, Lab Biochem & Mol Biol, Sao Carlos, SP, BrazilUniv Zagreb, Clin Internal Dis, ERA Chair Team VetMedZg, Fac Vet Med, Zagreb, CroatiaUniv Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP, BrazilAdolfo Lutz Inst, Ctr Reg Labs 2, Bauru, SP, BrazilPaulista Agcy Agribusiness Technol APTA, Bauru, SP, BrazilUniv Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Glasgow, Lanark, ScotlandSao Paulo State Univ UNESP, Grad Program Clin Res, Botucatu Med Sch FMB, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch FMB, Grad Program Trop Dis, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Grad Program Clin Res, Botucatu Med Sch FMB, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP, BrazilFAPESP: 2014/13299-7CNPq: 458919/2014-4CNPq: 563582/2010-3CAPES: 23038.006285/2011-21CAPES: 23038.008557/2010CNPq: 441463/2019-3Cevap-sao Paulo State Univ-unespUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Univ ZagrebUniversidade Estadual de Campinas (UNICAMP)Adolfo Lutz InstPaulista Agcy Agribusiness Technol APTAUniv GlasgowPontes, Leticia Gomes de [UNESP]Altei, Wanessa FernandaGalan, AsierBilic, PetraGuillemin, NicolasKules, JosipaHorvatic, AnitaMorais Ribeiro, Ligia Nunes dePaula, Eneida deRichini Pereira, Virginia BodelaoLucheis, Simone BaldiniMrljak, VladimirEckersall, Peter DavidFerreira, Rui Seabra [UNESP]Santos, Lucilene Delazari dos [UNESP]2020-12-10T20:01:08Z2020-12-10T20:01:08Z2020-05-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0067Journal Of Venomous Animals And Toxins Including Tropical Diseases. Botucatu: Cevap-sao Paulo State Univ-unesp, v. 26, 14 p., 2020.http://hdl.handle.net/11449/19694110.1590/1678-9199-JVATITD-2019-0067S1678-91992020000100314WOS:000537881000001S1678-91992020000100314.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Venomous Animals And Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-08-15T15:22:24Zoai:repositorio.unesp.br:11449/196941Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:22:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes |
title |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes |
spellingShingle |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes Pontes, Leticia Gomes de [UNESP] Extracellular vesicles Theileria spp. Protozoan parasites Nanoparticle tracking analysis Proteomic analysis |
title_short |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes |
title_full |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes |
title_fullStr |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes |
title_full_unstemmed |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes |
title_sort |
Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes |
author |
Pontes, Leticia Gomes de [UNESP] |
author_facet |
Pontes, Leticia Gomes de [UNESP] Altei, Wanessa Fernanda Galan, Asier Bilic, Petra Guillemin, Nicolas Kules, Josipa Horvatic, Anita Morais Ribeiro, Ligia Nunes de Paula, Eneida de Richini Pereira, Virginia Bodelao Lucheis, Simone Baldini Mrljak, Vladimir Eckersall, Peter David Ferreira, Rui Seabra [UNESP] Santos, Lucilene Delazari dos [UNESP] |
author_role |
author |
author2 |
Altei, Wanessa Fernanda Galan, Asier Bilic, Petra Guillemin, Nicolas Kules, Josipa Horvatic, Anita Morais Ribeiro, Ligia Nunes de Paula, Eneida de Richini Pereira, Virginia Bodelao Lucheis, Simone Baldini Mrljak, Vladimir Eckersall, Peter David Ferreira, Rui Seabra [UNESP] Santos, Lucilene Delazari dos [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) Univ Zagreb Universidade Estadual de Campinas (UNICAMP) Adolfo Lutz Inst Paulista Agcy Agribusiness Technol APTA Univ Glasgow |
dc.contributor.author.fl_str_mv |
Pontes, Leticia Gomes de [UNESP] Altei, Wanessa Fernanda Galan, Asier Bilic, Petra Guillemin, Nicolas Kules, Josipa Horvatic, Anita Morais Ribeiro, Ligia Nunes de Paula, Eneida de Richini Pereira, Virginia Bodelao Lucheis, Simone Baldini Mrljak, Vladimir Eckersall, Peter David Ferreira, Rui Seabra [UNESP] Santos, Lucilene Delazari dos [UNESP] |
dc.subject.por.fl_str_mv |
Extracellular vesicles Theileria spp. Protozoan parasites Nanoparticle tracking analysis Proteomic analysis |
topic |
Extracellular vesicles Theileria spp. Protozoan parasites Nanoparticle tracking analysis Proteomic analysis |
description |
Background: Extracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (Bubalus bubalis) sera infected with Theileria spp. Methods: Infected animals were identified through optical microscopy and PCR. EVs were isolated from buffalo sera by size-exclusion chromatography and characterized using western blotting analysis, nanoparticle tracking analysis and transmission electron microscopy. Subsequently, the proteins from isolated vesicles were characterized by mass spectrometry. Results: EVs from buffalo sera have shown sizes in the 124-140 nm range and 306 proteins were characterized. The protein-protein interaction analysis has evidenced biological processes and molecular function associated with signal transduction, binding, regulation of metabolic processes, transport, catalytic activity and response to acute stress. Five proteins have been shown to be differentially expressed between the control group and that infected with Theileria spp., all acting in the oxidative stress pathway. Conclusions: EVs from buffaloes infected with Theileria spp. were successfully isolated and characterized. This is an advance in the knowledge of host-parasite relationship that contributes to the understanding of host immune response and theileriosis evasion mechanisms. These findings may pave the way for searching new EVs candidate-markers for a better production of safe biological products derived from buffaloes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:01:08Z 2020-12-10T20:01:08Z 2020-05-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0067 Journal Of Venomous Animals And Toxins Including Tropical Diseases. Botucatu: Cevap-sao Paulo State Univ-unesp, v. 26, 14 p., 2020. http://hdl.handle.net/11449/196941 10.1590/1678-9199-JVATITD-2019-0067 S1678-91992020000100314 WOS:000537881000001 S1678-91992020000100314.pdf |
url |
http://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0067 http://hdl.handle.net/11449/196941 |
identifier_str_mv |
Journal Of Venomous Animals And Toxins Including Tropical Diseases. Botucatu: Cevap-sao Paulo State Univ-unesp, v. 26, 14 p., 2020. 10.1590/1678-9199-JVATITD-2019-0067 S1678-91992020000100314 WOS:000537881000001 S1678-91992020000100314.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Venomous Animals And Toxins Including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 application/pdf |
dc.publisher.none.fl_str_mv |
Cevap-sao Paulo State Univ-unesp |
publisher.none.fl_str_mv |
Cevap-sao Paulo State Univ-unesp |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128103476625408 |