Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cbpc.2019.05.005 http://hdl.handle.net/11449/185951 |
Resumo: | This study investigates the combined effects of waterborne copper exposure and acute temperature change on oxygen consumption and the oxidative stress biomarkers, glutathione S-transferase (GST) and glutathione peroxidase (GPx), in the gills and hepatopancreas of the fiddler crab Minuca rapax. Crabs held at 25 degrees C were acclimated to 0 (control), 50, 250 or 500 mu g Cu L-1 for 21 days, and were then subjected to 15, 25 and 35 degrees C for 24 h. Aerial oxygen consumption rates of crabs in copper free media increased with increasing temperature from 15 to 35 degrees C, Q(10) values reaching approximate to 3. Crabs exposed to increasing copper concentrations exhibited variable responses, Q so values falling to approximate to 1.5. Copper had no effect on oxygen consumption at 25 degrees C. However, at 35 degrees C, rates decreased in a clear concentration-response manner in the copper exposed crabs, revealing impaired aerobic capability. At 15 degrees C, oxygen consumption rates increased with copper concentration, except, for a decrease at 500 mu g CuL-1. Gill GST activity was approximate to 2-fold that of the hepatopancreas, while hepatopancreas GPx activity was 3-fold that of the gills. Gill GST activities were reduced by copper exposure only at 25 degrees C while hepatopancreas GST activities were altered by copper at all temperatures. Hepatopancreas GST and GPx activities increased in crabs exposed to copper at 35 degrees C, revealing oxidative stress induction. Hepatopancreas GST and GPx activities were reduced in copper exposed crabs at 15 degrees C, suggesting a diminished capability to mitigate the effects of copper exposure at low temperature. These findings reveal that copper exposure increases oxygen consumption at low temperatures but decreases consumption at high temperature. Hepatopancreas GPx activities decreased at low temperature and increased at high temperature. These novel findings demonstrate that the interaction between copper exposure and temperature should be considered when evaluating biomarker activities in semi-terrestrial crabs. |
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Repositório Institucional da UNESP |
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Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae)Oxygen consumptionAnti-oxidant activitiesBiomarkersCopper exposureTemperature effectsGelasiminaeSemi-terrestrial fiddler crabThis study investigates the combined effects of waterborne copper exposure and acute temperature change on oxygen consumption and the oxidative stress biomarkers, glutathione S-transferase (GST) and glutathione peroxidase (GPx), in the gills and hepatopancreas of the fiddler crab Minuca rapax. Crabs held at 25 degrees C were acclimated to 0 (control), 50, 250 or 500 mu g Cu L-1 for 21 days, and were then subjected to 15, 25 and 35 degrees C for 24 h. Aerial oxygen consumption rates of crabs in copper free media increased with increasing temperature from 15 to 35 degrees C, Q(10) values reaching approximate to 3. Crabs exposed to increasing copper concentrations exhibited variable responses, Q so values falling to approximate to 1.5. Copper had no effect on oxygen consumption at 25 degrees C. However, at 35 degrees C, rates decreased in a clear concentration-response manner in the copper exposed crabs, revealing impaired aerobic capability. At 15 degrees C, oxygen consumption rates increased with copper concentration, except, for a decrease at 500 mu g CuL-1. Gill GST activity was approximate to 2-fold that of the hepatopancreas, while hepatopancreas GPx activity was 3-fold that of the gills. Gill GST activities were reduced by copper exposure only at 25 degrees C while hepatopancreas GST activities were altered by copper at all temperatures. Hepatopancreas GST and GPx activities increased in crabs exposed to copper at 35 degrees C, revealing oxidative stress induction. Hepatopancreas GST and GPx activities were reduced in copper exposed crabs at 15 degrees C, suggesting a diminished capability to mitigate the effects of copper exposure at low temperature. These findings reveal that copper exposure increases oxygen consumption at low temperatures but decreases consumption at high temperature. Hepatopancreas GPx activities decreased at low temperature and increased at high temperature. These novel findings demonstrate that the interaction between copper exposure and temperature should be considered when evaluating biomarker activities in semi-terrestrial crabs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Campus Sao Vicente, BR-11380972 Sao Vicente, SP, BrazilUniv Sao Paulo, Ctr Biol Marinha, BR-11600000 Sao Sebastiao, SP, BrazilUniv Reg Amazon IKIAM, Via Tena,Muyuna Kilometro 7, Tena, Napo, EcuadorUniv Estadual Paulista, Campus Sao Vicente, BR-11380972 Sao Vicente, SP, BrazilFAPESP: 2011/22537-0FAPESP: 2011/08065-9CNPq: 300662/2009-2CNPq: 308649/2011CAPES: 33002029031P8CAPES: 001Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ Reg Amazon IKIAMCapparelli, Mariana VellosaBordon, Isabella C. [UNESP]Araujo, Giuliana [UNESP]Gusso-Choueri, Paloma Kachel [UNESP]Souza Abessa, Denis Moledo de [UNESP]McNamara, John Campbell2019-10-04T12:40:00Z2019-10-04T12:40:00Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article35-41http://dx.doi.org/10.1016/j.cbpc.2019.05.005Comparative Biochemistry And Physiology C-toxicology & Pharmacology. New York: Elsevier Science Inc, v. 223, p. 35-41, 2019.1532-0456http://hdl.handle.net/11449/18595110.1016/j.cbpc.2019.05.005WOS:000477686100005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Biochemistry And Physiology C-toxicology & Pharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T19:23:36Zoai:repositorio.unesp.br:11449/185951Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:44:27.004478Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) |
title |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) |
spellingShingle |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) Capparelli, Mariana Vellosa Oxygen consumption Anti-oxidant activities Biomarkers Copper exposure Temperature effects Gelasiminae Semi-terrestrial fiddler crab |
title_short |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) |
title_full |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) |
title_fullStr |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) |
title_full_unstemmed |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) |
title_sort |
Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae) |
author |
Capparelli, Mariana Vellosa |
author_facet |
Capparelli, Mariana Vellosa Bordon, Isabella C. [UNESP] Araujo, Giuliana [UNESP] Gusso-Choueri, Paloma Kachel [UNESP] Souza Abessa, Denis Moledo de [UNESP] McNamara, John Campbell |
author_role |
author |
author2 |
Bordon, Isabella C. [UNESP] Araujo, Giuliana [UNESP] Gusso-Choueri, Paloma Kachel [UNESP] Souza Abessa, Denis Moledo de [UNESP] McNamara, John Campbell |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Reg Amazon IKIAM |
dc.contributor.author.fl_str_mv |
Capparelli, Mariana Vellosa Bordon, Isabella C. [UNESP] Araujo, Giuliana [UNESP] Gusso-Choueri, Paloma Kachel [UNESP] Souza Abessa, Denis Moledo de [UNESP] McNamara, John Campbell |
dc.subject.por.fl_str_mv |
Oxygen consumption Anti-oxidant activities Biomarkers Copper exposure Temperature effects Gelasiminae Semi-terrestrial fiddler crab |
topic |
Oxygen consumption Anti-oxidant activities Biomarkers Copper exposure Temperature effects Gelasiminae Semi-terrestrial fiddler crab |
description |
This study investigates the combined effects of waterborne copper exposure and acute temperature change on oxygen consumption and the oxidative stress biomarkers, glutathione S-transferase (GST) and glutathione peroxidase (GPx), in the gills and hepatopancreas of the fiddler crab Minuca rapax. Crabs held at 25 degrees C were acclimated to 0 (control), 50, 250 or 500 mu g Cu L-1 for 21 days, and were then subjected to 15, 25 and 35 degrees C for 24 h. Aerial oxygen consumption rates of crabs in copper free media increased with increasing temperature from 15 to 35 degrees C, Q(10) values reaching approximate to 3. Crabs exposed to increasing copper concentrations exhibited variable responses, Q so values falling to approximate to 1.5. Copper had no effect on oxygen consumption at 25 degrees C. However, at 35 degrees C, rates decreased in a clear concentration-response manner in the copper exposed crabs, revealing impaired aerobic capability. At 15 degrees C, oxygen consumption rates increased with copper concentration, except, for a decrease at 500 mu g CuL-1. Gill GST activity was approximate to 2-fold that of the hepatopancreas, while hepatopancreas GPx activity was 3-fold that of the gills. Gill GST activities were reduced by copper exposure only at 25 degrees C while hepatopancreas GST activities were altered by copper at all temperatures. Hepatopancreas GST and GPx activities increased in crabs exposed to copper at 35 degrees C, revealing oxidative stress induction. Hepatopancreas GST and GPx activities were reduced in copper exposed crabs at 15 degrees C, suggesting a diminished capability to mitigate the effects of copper exposure at low temperature. These findings reveal that copper exposure increases oxygen consumption at low temperatures but decreases consumption at high temperature. Hepatopancreas GPx activities decreased at low temperature and increased at high temperature. These novel findings demonstrate that the interaction between copper exposure and temperature should be considered when evaluating biomarker activities in semi-terrestrial crabs. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:40:00Z 2019-10-04T12:40:00Z 2019-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cbpc.2019.05.005 Comparative Biochemistry And Physiology C-toxicology & Pharmacology. New York: Elsevier Science Inc, v. 223, p. 35-41, 2019. 1532-0456 http://hdl.handle.net/11449/185951 10.1016/j.cbpc.2019.05.005 WOS:000477686100005 |
url |
http://dx.doi.org/10.1016/j.cbpc.2019.05.005 http://hdl.handle.net/11449/185951 |
identifier_str_mv |
Comparative Biochemistry And Physiology C-toxicology & Pharmacology. New York: Elsevier Science Inc, v. 223, p. 35-41, 2019. 1532-0456 10.1016/j.cbpc.2019.05.005 WOS:000477686100005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Biochemistry And Physiology C-toxicology & Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
35-41 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128270161412096 |