Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201601454 http://hdl.handle.net/11449/175005 |
Resumo: | Innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32×, 64×, 125× and 320× magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals. |
id |
UNSP_50ed250d7b431337b6cbaed31881e32f |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/175005 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cementBiocompatible materialsBone regenerationCalcium aluminate cementInflammationInnovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32×, 64×, 125× and 320× magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Clinics and Surgery Jaboticabal School of Agricultural and Veterinary Sciences UNESP - Universidade Estadual PaulistaDepartment of Pathology Ribeirão Preto School of Medicine USP - Universidade de São PauloDepartment of Physiology and Pathology Araraquara Dental School UNESP - Universidade Estadual PaulistaBinderware Biomaterials LtdaDepartment of Dental Materials and Prosthodontics Ribeirão Preto Dental School USP - Universidade de São PauloDepartment of Dentistry - Endodontics Division Health Sciences Center UFSC - Universidade Federal de Santa CatarinaDepartment of Clinics and Surgery Jaboticabal School of Agricultural and Veterinary Sciences UNESP - Universidade Estadual PaulistaDepartment of Physiology and Pathology Araraquara Dental School UNESP - Universidade Estadual PaulistaCNPq: #303599/2014-6CNPq: #443153/2014-0CNPq: #67/2008Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Binderware Biomaterials LtdaUniversidade Federal de Santa Catarina (UFSC)de Moraes, Paola Castro [UNESP]Marques, Isabela Cristina de SouzaBasso, Fernanda Gonçalves [UNESP]Rossetto, Hebert LuisPiresde-Souza, Fernanda de Carvalho PanzeriCosta, Carlos Alberto de Souza [UNESP]Garcia, Lucas da Fonseca Roberti2018-12-11T17:13:49Z2018-12-11T17:13:49Z2017-08-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article287-295application/pdfhttp://dx.doi.org/10.1590/0103-6440201601454Brazilian Dental Journal, v. 28, n. 3, p. 287-295, 2017.1806-47600103-6440http://hdl.handle.net/11449/17500510.1590/0103-6440201601454S0103-644020170003002872-s2.0-85026828838S0103-64402017000300287.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2024-06-06T14:10:22Zoai:repositorio.unesp.br:11449/175005Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-06T14:10:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement |
title |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement |
spellingShingle |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement de Moraes, Paola Castro [UNESP] Biocompatible materials Bone regeneration Calcium aluminate cement Inflammation |
title_short |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement |
title_full |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement |
title_fullStr |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement |
title_full_unstemmed |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement |
title_sort |
Repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement |
author |
de Moraes, Paola Castro [UNESP] |
author_facet |
de Moraes, Paola Castro [UNESP] Marques, Isabela Cristina de Souza Basso, Fernanda Gonçalves [UNESP] Rossetto, Hebert Luis Piresde-Souza, Fernanda de Carvalho Panzeri Costa, Carlos Alberto de Souza [UNESP] Garcia, Lucas da Fonseca Roberti |
author_role |
author |
author2 |
Marques, Isabela Cristina de Souza Basso, Fernanda Gonçalves [UNESP] Rossetto, Hebert Luis Piresde-Souza, Fernanda de Carvalho Panzeri Costa, Carlos Alberto de Souza [UNESP] Garcia, Lucas da Fonseca Roberti |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Binderware Biomaterials Ltda Universidade Federal de Santa Catarina (UFSC) |
dc.contributor.author.fl_str_mv |
de Moraes, Paola Castro [UNESP] Marques, Isabela Cristina de Souza Basso, Fernanda Gonçalves [UNESP] Rossetto, Hebert Luis Piresde-Souza, Fernanda de Carvalho Panzeri Costa, Carlos Alberto de Souza [UNESP] Garcia, Lucas da Fonseca Roberti |
dc.subject.por.fl_str_mv |
Biocompatible materials Bone regeneration Calcium aluminate cement Inflammation |
topic |
Biocompatible materials Bone regeneration Calcium aluminate cement Inflammation |
description |
Innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32×, 64×, 125× and 320× magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-02 2018-12-11T17:13:49Z 2018-12-11T17:13:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201601454 Brazilian Dental Journal, v. 28, n. 3, p. 287-295, 2017. 1806-4760 0103-6440 http://hdl.handle.net/11449/175005 10.1590/0103-6440201601454 S0103-64402017000300287 2-s2.0-85026828838 S0103-64402017000300287.pdf |
url |
http://dx.doi.org/10.1590/0103-6440201601454 http://hdl.handle.net/11449/175005 |
identifier_str_mv |
Brazilian Dental Journal, v. 28, n. 3, p. 287-295, 2017. 1806-4760 0103-6440 10.1590/0103-6440201601454 S0103-64402017000300287 2-s2.0-85026828838 S0103-64402017000300287.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
287-295 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045426883985408 |