Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2016000200002 http://hdl.handle.net/11449/168576 |
Resumo: | Dermatosparaxis is an autosomal recessive disorder of connective tissue; the disorder is clinically characterized by skin fragility and hyperextensibility. Dermatosparaxis in White Dorper sheep is caused by a single nucleotide polymorphism (SNP) (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. The aim of this study was to investigate the prevalence of this SNP in a White Dorper herd in São Paulo state, Brazil. In this study, we collected blood DNA samples from 303 White Dorper sheep and performed polymerase chain reaction to amplify the SNP region. The samples were sequenced to determine the presence of the SNP in the ADAMTS2 gene. The SNP prevalence in the studied population was 15.5%; this finding indicates that more effective control measures should be used to prevent the inheritance of SNP c.421G>T in the ADAMTS2 gene in Brazilian White Dorper herds. |
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Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in BrazilAutosomal recessiveCutaneous astheniaPCRSheepWhite dorperDermatosparaxis is an autosomal recessive disorder of connective tissue; the disorder is clinically characterized by skin fragility and hyperextensibility. Dermatosparaxis in White Dorper sheep is caused by a single nucleotide polymorphism (SNP) (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. The aim of this study was to investigate the prevalence of this SNP in a White Dorper herd in São Paulo state, Brazil. In this study, we collected blood DNA samples from 303 White Dorper sheep and performed polymerase chain reaction to amplify the SNP region. The samples were sequenced to determine the presence of the SNP in the ADAMTS2 gene. The SNP prevalence in the studied population was 15.5%; this finding indicates that more effective control measures should be used to prevent the inheritance of SNP c.421G>T in the ADAMTS2 gene in Brazilian White Dorper herds.Departamento de Clínicas Veterinárias Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Campus de Botucatu, Distrito de Rubiao Junior s/nDepartamento de Medicina Veterinária Escola de Veterinária Universidade Federal de Goiás (UFG), Campus Samambaia (Campus II), Cx. Postal 131Departamento de Clínicas Veterinárias Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Campus de Botucatu, Distrito de Rubiao Junior s/nUniversidade Estadual Paulista (Unesp)Universidade Federal de Goiás (UFG)Andrade, Danilo G.A. [UNESP]Dalanezi, Felipe M. [UNESP]Trecenti, Anelize S. [UNESP]Cunha, Paulo Henrique J.Borges, Alexandre S. [UNESP]Oliveira-Filho, José P. [UNESP]2018-12-11T16:42:00Z2018-12-11T16:42:00Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article73-76application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016000200002Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 73-76, 2016.1678-51500100-736Xhttp://hdl.handle.net/11449/16857610.1590/S0100-736X2016000200002S0100-736X20160002000732-s2.0-84963769368S0100-736X2016000200073.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-12-24T06:14:56Zoai:repositorio.unesp.br:11449/168576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-24T06:14:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil |
title |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil |
spellingShingle |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil Andrade, Danilo G.A. [UNESP] Autosomal recessive Cutaneous asthenia PCR Sheep White dorper |
title_short |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil |
title_full |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil |
title_fullStr |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil |
title_full_unstemmed |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil |
title_sort |
Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil |
author |
Andrade, Danilo G.A. [UNESP] |
author_facet |
Andrade, Danilo G.A. [UNESP] Dalanezi, Felipe M. [UNESP] Trecenti, Anelize S. [UNESP] Cunha, Paulo Henrique J. Borges, Alexandre S. [UNESP] Oliveira-Filho, José P. [UNESP] |
author_role |
author |
author2 |
Dalanezi, Felipe M. [UNESP] Trecenti, Anelize S. [UNESP] Cunha, Paulo Henrique J. Borges, Alexandre S. [UNESP] Oliveira-Filho, José P. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Goiás (UFG) |
dc.contributor.author.fl_str_mv |
Andrade, Danilo G.A. [UNESP] Dalanezi, Felipe M. [UNESP] Trecenti, Anelize S. [UNESP] Cunha, Paulo Henrique J. Borges, Alexandre S. [UNESP] Oliveira-Filho, José P. [UNESP] |
dc.subject.por.fl_str_mv |
Autosomal recessive Cutaneous asthenia PCR Sheep White dorper |
topic |
Autosomal recessive Cutaneous asthenia PCR Sheep White dorper |
description |
Dermatosparaxis is an autosomal recessive disorder of connective tissue; the disorder is clinically characterized by skin fragility and hyperextensibility. Dermatosparaxis in White Dorper sheep is caused by a single nucleotide polymorphism (SNP) (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. The aim of this study was to investigate the prevalence of this SNP in a White Dorper herd in São Paulo state, Brazil. In this study, we collected blood DNA samples from 303 White Dorper sheep and performed polymerase chain reaction to amplify the SNP region. The samples were sequenced to determine the presence of the SNP in the ADAMTS2 gene. The SNP prevalence in the studied population was 15.5%; this finding indicates that more effective control measures should be used to prevent the inheritance of SNP c.421G>T in the ADAMTS2 gene in Brazilian White Dorper herds. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-01 2018-12-11T16:42:00Z 2018-12-11T16:42:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2016000200002 Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 73-76, 2016. 1678-5150 0100-736X http://hdl.handle.net/11449/168576 10.1590/S0100-736X2016000200002 S0100-736X2016000200073 2-s2.0-84963769368 S0100-736X2016000200073.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2016000200002 http://hdl.handle.net/11449/168576 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 73-76, 2016. 1678-5150 0100-736X 10.1590/S0100-736X2016000200002 S0100-736X2016000200073 2-s2.0-84963769368 S0100-736X2016000200073.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
73-76 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803047089322590208 |