Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1365-3083.2011.02510.x http://hdl.handle.net/11449/18287 |
Resumo: | This work investigated the effect of previous Mycobacterium avium exposure on the protective ability of the DNA vaccine pVAXhsp65 against inflammation in the pulmonary parenchyma. BALB/c mice were presensitized with heat-killed M. avium and then immunized with three doses of pVAXhsp65 prior to challenge with Mycobacterium tuberculosis. M. avium sensitization induced high levels of spontaneous IL-5 production that were concomitant with a positive delayed-type hypersensitivity reaction; antigen-specific IFN-gamma production was also observed upon splenocyte stimulation. Prior exposure to M. avium resulted in altered cytokine and antibody production induced by immunization with pVAXhsp65; instead of a Th1 response, vaccinated mice previously exposed to M. avium developed a strong Th2 response. This switch to a Th2 response coincided with the loss of the anti-inflammatory effect of pVAXhsp65 vaccination previously observed in the pulmonary parenchyma of mice infected with M. tuberculosis. These results suggest that exposure to environmental mycobacteria can modulate immune responses induced by mycobacterial vaccines other than bacillus Calmette-Guerin. |
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Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary ParenchymaThis work investigated the effect of previous Mycobacterium avium exposure on the protective ability of the DNA vaccine pVAXhsp65 against inflammation in the pulmonary parenchyma. BALB/c mice were presensitized with heat-killed M. avium and then immunized with three doses of pVAXhsp65 prior to challenge with Mycobacterium tuberculosis. M. avium sensitization induced high levels of spontaneous IL-5 production that were concomitant with a positive delayed-type hypersensitivity reaction; antigen-specific IFN-gamma production was also observed upon splenocyte stimulation. Prior exposure to M. avium resulted in altered cytokine and antibody production induced by immunization with pVAXhsp65; instead of a Th1 response, vaccinated mice previously exposed to M. avium developed a strong Th2 response. This switch to a Th2 response coincided with the loss of the anti-inflammatory effect of pVAXhsp65 vaccination previously observed in the pulmonary parenchyma of mice infected with M. tuberculosis. These results suggest that exposure to environmental mycobacteria can modulate immune responses induced by mycobacterial vaccines other than bacillus Calmette-Guerin.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Microbiol & Immunol, Biosci Inst, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Dept Biochem & Immunol, BR-18618000 São Paulo, BrazilUFMT, Inst Biol & Nat Sci, Uberaba, MG, BrazilUniv Estadual Paulista, Dept Microbiol & Immunol, Biosci Inst, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Dept Biochem & Immunol, BR-18618000 São Paulo, BrazilWiley-BlackwellUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso (UFMT)Martins, D. R. [UNESP]Pelizon, A. C. [UNESP]Zorzella-Pezavento, S. F. G. [UNESP]Seger, J. [UNESP]Santos Junior, R. R. [UNESP]Fonseca, D. M. [UNESP]Justulin, L. A.Silva, C. L. [UNESP]Sartori, Alexandrina [UNESP]2014-05-20T13:51:13Z2014-05-20T13:51:13Z2011-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article293-300application/pdfhttp://dx.doi.org/10.1111/j.1365-3083.2011.02510.xScandinavian Journal of Immunology. Malden: Wiley-blackwell, v. 73, n. 4, p. 293-300, 2011.0300-9475http://hdl.handle.net/11449/1828710.1111/j.1365-3083.2011.02510.xWOS:000287871800004WOS000287871800004.pdf4977572416129527Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScandinavian Journal of Immunology2.3140,891info:eu-repo/semantics/openAccess2023-11-23T06:18:13Zoai:repositorio.unesp.br:11449/18287Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-23T06:18:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma |
title |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma |
spellingShingle |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma Martins, D. R. [UNESP] |
title_short |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma |
title_full |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma |
title_fullStr |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma |
title_full_unstemmed |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma |
title_sort |
Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma |
author |
Martins, D. R. [UNESP] |
author_facet |
Martins, D. R. [UNESP] Pelizon, A. C. [UNESP] Zorzella-Pezavento, S. F. G. [UNESP] Seger, J. [UNESP] Santos Junior, R. R. [UNESP] Fonseca, D. M. [UNESP] Justulin, L. A. Silva, C. L. [UNESP] Sartori, Alexandrina [UNESP] |
author_role |
author |
author2 |
Pelizon, A. C. [UNESP] Zorzella-Pezavento, S. F. G. [UNESP] Seger, J. [UNESP] Santos Junior, R. R. [UNESP] Fonseca, D. M. [UNESP] Justulin, L. A. Silva, C. L. [UNESP] Sartori, Alexandrina [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso (UFMT) |
dc.contributor.author.fl_str_mv |
Martins, D. R. [UNESP] Pelizon, A. C. [UNESP] Zorzella-Pezavento, S. F. G. [UNESP] Seger, J. [UNESP] Santos Junior, R. R. [UNESP] Fonseca, D. M. [UNESP] Justulin, L. A. Silva, C. L. [UNESP] Sartori, Alexandrina [UNESP] |
description |
This work investigated the effect of previous Mycobacterium avium exposure on the protective ability of the DNA vaccine pVAXhsp65 against inflammation in the pulmonary parenchyma. BALB/c mice were presensitized with heat-killed M. avium and then immunized with three doses of pVAXhsp65 prior to challenge with Mycobacterium tuberculosis. M. avium sensitization induced high levels of spontaneous IL-5 production that were concomitant with a positive delayed-type hypersensitivity reaction; antigen-specific IFN-gamma production was also observed upon splenocyte stimulation. Prior exposure to M. avium resulted in altered cytokine and antibody production induced by immunization with pVAXhsp65; instead of a Th1 response, vaccinated mice previously exposed to M. avium developed a strong Th2 response. This switch to a Th2 response coincided with the loss of the anti-inflammatory effect of pVAXhsp65 vaccination previously observed in the pulmonary parenchyma of mice infected with M. tuberculosis. These results suggest that exposure to environmental mycobacteria can modulate immune responses induced by mycobacterial vaccines other than bacillus Calmette-Guerin. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-01 2014-05-20T13:51:13Z 2014-05-20T13:51:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1365-3083.2011.02510.x Scandinavian Journal of Immunology. Malden: Wiley-blackwell, v. 73, n. 4, p. 293-300, 2011. 0300-9475 http://hdl.handle.net/11449/18287 10.1111/j.1365-3083.2011.02510.x WOS:000287871800004 WOS000287871800004.pdf 4977572416129527 |
url |
http://dx.doi.org/10.1111/j.1365-3083.2011.02510.x http://hdl.handle.net/11449/18287 |
identifier_str_mv |
Scandinavian Journal of Immunology. Malden: Wiley-blackwell, v. 73, n. 4, p. 293-300, 2011. 0300-9475 10.1111/j.1365-3083.2011.02510.x WOS:000287871800004 WOS000287871800004.pdf 4977572416129527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scandinavian Journal of Immunology 2.314 0,891 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
293-300 application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965047263330304 |