Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2341/12-464-L http://hdl.handle.net/11449/112988 |
Resumo: | The aim of this study was to evaluate the fluorescence intensity of different composite resins and compare those values with the fluorescence intensity of dental tissues. Different composite resins were used to make 10 discs (2 mm in depth and 4 mm in diameter) of each brand, divided into groups: 1) Z (Filtek Z350, 3M ESPE), 2) ES (Esthet-X, Dentsply), 3) A (Amelogen Plus, Ultradent), 4) DVS (Durafill-VS, Heraeus Kulzer) with 2 mm composite resin for enamel (A2), 5) OES ([Esthet-X] opaque-OA [1 mm] + enamel-A2 [1 mm]); 6) ODVSI ([Charisma-Opal/Durafill-VSI], opaque-OM (1 mm) + translucent [1mm]), and 7) DVSI ([Durafill- VSI] translucent [2 mm]). Dental tissue specimens were obtained from human anterior teeth cut in a mesiodistal direction to obtain enamel, dentin, and enamel/dentin samples (2 mm). The fluorescence intensity of specimens was directly measured using an optic fiber associated with a spectrometer (Ocean Optics USB 4000) and recorded in graphic form (Origin 8.0 program). Data were submitted to statistical analysis using Dunnet, Tukey, and Kruskall-Wallis tests. Light absorption of the composite resins was obtained in a spectral range from 250 to 450 nm, and that of dental tissues was between 250 and 300 nm. All composite resins were excited at 398 nm and exhibited maximum emissions of around 485 mu. Fluorescence intensity values for all of the resins showed statistically significant differences (measured in arbitrary units [AUs]), with the exception of groups Z and DVS. Group DVSI had the highest fluorescence intensity values (13539 AU), followed by ODVS (10440 AU), DVS (10146 AU), ES (3946 AU), OES (3841 AU), A (3540 AU), and Z (1146 AU). The fluorescence intensity values for the composite resins differed statistically from those of dental tissues (E=1380 AU; D=6262 AU; E/D=3251 AU). The opacity interfered with fluorescence intensity, and group Z demonstrated fluorescence intensity values closest to that of tooth enamel. It is concluded that the fluorescence intensity values were significantly different among the composite resins and compared with dental tissues. The direct spectrofluorimetric method represents a tool for evaluating the fluorescence of composite resins. |
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Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental TissuesThe aim of this study was to evaluate the fluorescence intensity of different composite resins and compare those values with the fluorescence intensity of dental tissues. Different composite resins were used to make 10 discs (2 mm in depth and 4 mm in diameter) of each brand, divided into groups: 1) Z (Filtek Z350, 3M ESPE), 2) ES (Esthet-X, Dentsply), 3) A (Amelogen Plus, Ultradent), 4) DVS (Durafill-VS, Heraeus Kulzer) with 2 mm composite resin for enamel (A2), 5) OES ([Esthet-X] opaque-OA [1 mm] + enamel-A2 [1 mm]); 6) ODVSI ([Charisma-Opal/Durafill-VSI], opaque-OM (1 mm) + translucent [1mm]), and 7) DVSI ([Durafill- VSI] translucent [2 mm]). Dental tissue specimens were obtained from human anterior teeth cut in a mesiodistal direction to obtain enamel, dentin, and enamel/dentin samples (2 mm). The fluorescence intensity of specimens was directly measured using an optic fiber associated with a spectrometer (Ocean Optics USB 4000) and recorded in graphic form (Origin 8.0 program). Data were submitted to statistical analysis using Dunnet, Tukey, and Kruskall-Wallis tests. Light absorption of the composite resins was obtained in a spectral range from 250 to 450 nm, and that of dental tissues was between 250 and 300 nm. All composite resins were excited at 398 nm and exhibited maximum emissions of around 485 mu. Fluorescence intensity values for all of the resins showed statistically significant differences (measured in arbitrary units [AUs]), with the exception of groups Z and DVS. Group DVSI had the highest fluorescence intensity values (13539 AU), followed by ODVS (10440 AU), DVS (10146 AU), ES (3946 AU), OES (3841 AU), A (3540 AU), and Z (1146 AU). The fluorescence intensity values for the composite resins differed statistically from those of dental tissues (E=1380 AU; D=6262 AU; E/D=3251 AU). The opacity interfered with fluorescence intensity, and group Z demonstrated fluorescence intensity values closest to that of tooth enamel. It is concluded that the fluorescence intensity values were significantly different among the composite resins and compared with dental tissues. The direct spectrofluorimetric method represents a tool for evaluating the fluorescence of composite resins.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Dept Restorat Dent, Sao Jose Dos Campos, BrazilUniv Pelotas, Ctr Chem Sci Pharmaceut & Food, Sao Jose Dos Campos, BrazilUniv Camilo Castelo Branco, Sao Jose Dos Campos, BrazilUniv Estadual Paulista, UNESP, Social Dent & Childrens Clin Dept, Sao Jose Dos Campos, BrazilSao Paulo State Univ, UNESP, Dept Restorat Dent, Sao Jose dos Campos Dent Sch, Sao Jose Dos Campos, BrazilSao Paulo State Univ, UNESP, Sao Jose dos Campos Dent Sch, Sao Jose Dos Campos, BrazilUniv Estadual Paulista, UNESP, Dept Restorat Dent, Sao Jose Dos Campos, BrazilUniv Estadual Paulista, UNESP, Social Dent & Childrens Clin Dept, Sao Jose Dos Campos, BrazilSao Paulo State Univ, UNESP, Dept Restorat Dent, Sao Jose dos Campos Dent Sch, Sao Jose Dos Campos, BrazilSao Paulo State Univ, UNESP, Sao Jose dos Campos Dent Sch, Sao Jose Dos Campos, BrazilFAPESP: 10/50834-7Operative Dentistry IncUniversidade Estadual Paulista (Unesp)Univ PelotasUniv Camilo Castelo BrancoSilva, T. M. da [UNESP]Oliveira, H. P. M. deSeverino, D.Balducci, I. [UNESP]Huhtala, M. F. R. L. [UNESP]Goncalves, S. E. P. [UNESP]2014-12-03T13:11:12Z2014-12-03T13:11:12Z2014-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article407-415application/pdfhttp://dx.doi.org/10.2341/12-464-LOperative Dentistry. Indianapolis: Operative Dentistry Inc, v. 39, n. 4, p. 407-415, 2014.0361-7734http://hdl.handle.net/11449/11298810.2341/12-464-LWOS:000338095100010WOS000338095100010.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOperative Dentistry2.1301,180info:eu-repo/semantics/openAccess2023-10-19T06:11:11Zoai:repositorio.unesp.br:11449/112988Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-19T06:11:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues |
title |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues |
spellingShingle |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues Silva, T. M. da [UNESP] |
title_short |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues |
title_full |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues |
title_fullStr |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues |
title_full_unstemmed |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues |
title_sort |
Direct Spectrometry: A New Alternative for Measuring the Fluorescence of Composite Resins and Dental Tissues |
author |
Silva, T. M. da [UNESP] |
author_facet |
Silva, T. M. da [UNESP] Oliveira, H. P. M. de Severino, D. Balducci, I. [UNESP] Huhtala, M. F. R. L. [UNESP] Goncalves, S. E. P. [UNESP] |
author_role |
author |
author2 |
Oliveira, H. P. M. de Severino, D. Balducci, I. [UNESP] Huhtala, M. F. R. L. [UNESP] Goncalves, S. E. P. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Pelotas Univ Camilo Castelo Branco |
dc.contributor.author.fl_str_mv |
Silva, T. M. da [UNESP] Oliveira, H. P. M. de Severino, D. Balducci, I. [UNESP] Huhtala, M. F. R. L. [UNESP] Goncalves, S. E. P. [UNESP] |
description |
The aim of this study was to evaluate the fluorescence intensity of different composite resins and compare those values with the fluorescence intensity of dental tissues. Different composite resins were used to make 10 discs (2 mm in depth and 4 mm in diameter) of each brand, divided into groups: 1) Z (Filtek Z350, 3M ESPE), 2) ES (Esthet-X, Dentsply), 3) A (Amelogen Plus, Ultradent), 4) DVS (Durafill-VS, Heraeus Kulzer) with 2 mm composite resin for enamel (A2), 5) OES ([Esthet-X] opaque-OA [1 mm] + enamel-A2 [1 mm]); 6) ODVSI ([Charisma-Opal/Durafill-VSI], opaque-OM (1 mm) + translucent [1mm]), and 7) DVSI ([Durafill- VSI] translucent [2 mm]). Dental tissue specimens were obtained from human anterior teeth cut in a mesiodistal direction to obtain enamel, dentin, and enamel/dentin samples (2 mm). The fluorescence intensity of specimens was directly measured using an optic fiber associated with a spectrometer (Ocean Optics USB 4000) and recorded in graphic form (Origin 8.0 program). Data were submitted to statistical analysis using Dunnet, Tukey, and Kruskall-Wallis tests. Light absorption of the composite resins was obtained in a spectral range from 250 to 450 nm, and that of dental tissues was between 250 and 300 nm. All composite resins were excited at 398 nm and exhibited maximum emissions of around 485 mu. Fluorescence intensity values for all of the resins showed statistically significant differences (measured in arbitrary units [AUs]), with the exception of groups Z and DVS. Group DVSI had the highest fluorescence intensity values (13539 AU), followed by ODVS (10440 AU), DVS (10146 AU), ES (3946 AU), OES (3841 AU), A (3540 AU), and Z (1146 AU). The fluorescence intensity values for the composite resins differed statistically from those of dental tissues (E=1380 AU; D=6262 AU; E/D=3251 AU). The opacity interfered with fluorescence intensity, and group Z demonstrated fluorescence intensity values closest to that of tooth enamel. It is concluded that the fluorescence intensity values were significantly different among the composite resins and compared with dental tissues. The direct spectrofluorimetric method represents a tool for evaluating the fluorescence of composite resins. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:11:12Z 2014-12-03T13:11:12Z 2014-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2341/12-464-L Operative Dentistry. Indianapolis: Operative Dentistry Inc, v. 39, n. 4, p. 407-415, 2014. 0361-7734 http://hdl.handle.net/11449/112988 10.2341/12-464-L WOS:000338095100010 WOS000338095100010.pdf |
url |
http://dx.doi.org/10.2341/12-464-L http://hdl.handle.net/11449/112988 |
identifier_str_mv |
Operative Dentistry. Indianapolis: Operative Dentistry Inc, v. 39, n. 4, p. 407-415, 2014. 0361-7734 10.2341/12-464-L WOS:000338095100010 WOS000338095100010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Operative Dentistry 2.130 1,180 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
407-415 application/pdf |
dc.publisher.none.fl_str_mv |
Operative Dentistry Inc |
publisher.none.fl_str_mv |
Operative Dentistry Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649464719638528 |