Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide bone regeneration

• Autor(a) principal: Saska, Sybele [UNESP]
• Data de Publicação: 2017
• Outros Autores: Teixeira, Lucas Novaes, de Castro Raucci, Larissa Moreira Spinola, Scarel-Caminaga, Raquel Mantuaneli [UNESP], Franchi, Leonardo Pereira, dos Santos, Raquel Alves, Santagneli, Silvia Helena [UNESP], Capela, Marisa Veiga [UNESP], de Oliveira, Paulo Tambasco, Takahashi, Catarina Satie, Gaspar, Ana Maria Minarelli [UNESP], Messaddeq, Younès [UNESP], Ribeiro, Sidney José Lima [UNESP], Marchetto, Reinaldo [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.ijbiomac.2017.05.086; http://hdl.handle.net/11449/178878
• topic: Bacterial cellulose; Biopolymer; Bone regeneration; Composite; Peptide; Tissue engineering

Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome. Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes. The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis. SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite. The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype. Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap. Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects. These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.