Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats
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Publication Date: | 2013 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1186/1742-4933-10-8 http://hdl.handle.net/11449/74628 |
Summary: | It is now commonly accepted that chronic inflammation associated with obesity during aging induces insulin resistance in the liver. In the present study, we investigated whether the improvement in insulin sensitivity and insulin signaling, mediated by acute exercise, could be associated with modulation of protein-tyrosine phosphatase 1B (PTP-1B) in the liver of old rats. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45 min rest period. Sixteen hours after the exercise, the rats were sacrificed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the fat mass was increased in old rats. The reduction in glucose disappearance rate (Kitt) observed in aged rats was restored 16 h after exercise. Aging increased the content of PTP-1B and attenuated insulin signaling in the liver of rats, a phenomenon that was reversed by exercise. Aging rats also increased the IRβ/PTP-1B and IRS-1/PTP-1B association in the liver when compared with young rats. Conversely, in the liver of exercised old rats, IRβ/PTP-1B and IRS-1/PTP-1B association was markedly decreased. Moreover, in the hepatic tissue of old rats, the insulin signalling was decreased and PEPCK and G6Pase levels were increased when compared with young rats. Interestingly, 16 h after acute exercise, the PEPCK and G6Pase protein level were decreased in the old exercised group. These results provide new insights into the mechanisms by which exercise restores insulin signalling in liver during aging. © 2013 Moura et al; licensee BioMed Central Ltd. |
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Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old ratsglucoseglucose 6 phosphataseinsulininsulin receptorinsulin receptor betainsulin receptor substrate 1protein tyrosine phosphatase 1Bunclassified drugaginganimal experimentcontrolled studydiet restrictionenzyme blood levelenzyme regulationepididymis fatexerciseglucose blood levelgroups by ageinsulin blood levelinsulin sensitivityinsulin tolerance testlivermolecular mechanicsnonhumanpriority journalratsignal transductionswimmingIt is now commonly accepted that chronic inflammation associated with obesity during aging induces insulin resistance in the liver. In the present study, we investigated whether the improvement in insulin sensitivity and insulin signaling, mediated by acute exercise, could be associated with modulation of protein-tyrosine phosphatase 1B (PTP-1B) in the liver of old rats. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45 min rest period. Sixteen hours after the exercise, the rats were sacrificed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the fat mass was increased in old rats. The reduction in glucose disappearance rate (Kitt) observed in aged rats was restored 16 h after exercise. Aging increased the content of PTP-1B and attenuated insulin signaling in the liver of rats, a phenomenon that was reversed by exercise. Aging rats also increased the IRβ/PTP-1B and IRS-1/PTP-1B association in the liver when compared with young rats. Conversely, in the liver of exercised old rats, IRβ/PTP-1B and IRS-1/PTP-1B association was markedly decreased. Moreover, in the hepatic tissue of old rats, the insulin signalling was decreased and PEPCK and G6Pase levels were increased when compared with young rats. Interestingly, 16 h after acute exercise, the PEPCK and G6Pase protein level were decreased in the old exercised group. These results provide new insights into the mechanisms by which exercise restores insulin signalling in liver during aging. © 2013 Moura et al; licensee BioMed Central Ltd.Universidade Estadual Paulista UNESP Curso de Pós-Graduação em Ciências da Motricidade Humana, Rio Claro, SPFaculdade de Ciências Aplicadas Universidade Estadual de Campinas Curso de Pós-Graduação em Nutrição, Esporte e Metabolismo. UNICAMP, Limeira, SPUniversidade do Extremo Sul Catarinense Laboratório de Bioquímica e Fisiologia, Santa Catarina, Criciúma, SCUniversidade de São Paulo Escola de Educação Física e Esporte USP, Ribeirão Preto, SPCurso de Ciências do Esporte FCA-UNICAMP, Rua Pedro Zaccaria, 1300, Jardim Santa Luzia, Limeira, SPUniversidade Estadual Paulista UNESP Curso de Pós-Graduação em Ciências da Motricidade Humana, Rio Claro, SPUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Laboratório de Bioquímica e FisiologiaUniversidade de São Paulo (USP)de Moura, Leandro Pereira [UNESP]Souza Pauli, Luciana SantosCintra, Dennys Esperde Souza, Claudio Teodoroda Silva, Adelino Sanchez RamosMarinho, Rodolfode Melo, Maria Alice Rostom [UNESP]Ropelle, Eduardo RochetePauli, José Rodrigo [UNESP]2014-05-27T11:28:33Z2014-05-27T11:28:33Z2013-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/1742-4933-10-8Immunity and Ageing, v. 10, n. 1, 2013.1742-4933http://hdl.handle.net/11449/7462810.1186/1742-4933-10-8WOS:0003272184000012-s2.0-848741503502-s2.0-84874150350.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengImmunity and Ageing4.0191,218info:eu-repo/semantics/openAccess2023-10-23T06:12:40Zoai:repositorio.unesp.br:11449/74628Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-23T06:12:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
spellingShingle |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats de Moura, Leandro Pereira [UNESP] glucose glucose 6 phosphatase insulin insulin receptor insulin receptor beta insulin receptor substrate 1 protein tyrosine phosphatase 1B unclassified drug aging animal experiment controlled study diet restriction enzyme blood level enzyme regulation epididymis fat exercise glucose blood level groups by age insulin blood level insulin sensitivity insulin tolerance test liver molecular mechanics nonhuman priority journal rat signal transduction swimming |
title_short |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_full |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_fullStr |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_full_unstemmed |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
title_sort |
Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats |
author |
de Moura, Leandro Pereira [UNESP] |
author_facet |
de Moura, Leandro Pereira [UNESP] Souza Pauli, Luciana Santos Cintra, Dennys Esper de Souza, Claudio Teodoro da Silva, Adelino Sanchez Ramos Marinho, Rodolfo de Melo, Maria Alice Rostom [UNESP] Ropelle, Eduardo Rochete Pauli, José Rodrigo [UNESP] |
author_role |
author |
author2 |
Souza Pauli, Luciana Santos Cintra, Dennys Esper de Souza, Claudio Teodoro da Silva, Adelino Sanchez Ramos Marinho, Rodolfo de Melo, Maria Alice Rostom [UNESP] Ropelle, Eduardo Rochete Pauli, José Rodrigo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Laboratório de Bioquímica e Fisiologia Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
de Moura, Leandro Pereira [UNESP] Souza Pauli, Luciana Santos Cintra, Dennys Esper de Souza, Claudio Teodoro da Silva, Adelino Sanchez Ramos Marinho, Rodolfo de Melo, Maria Alice Rostom [UNESP] Ropelle, Eduardo Rochete Pauli, José Rodrigo [UNESP] |
dc.subject.por.fl_str_mv |
glucose glucose 6 phosphatase insulin insulin receptor insulin receptor beta insulin receptor substrate 1 protein tyrosine phosphatase 1B unclassified drug aging animal experiment controlled study diet restriction enzyme blood level enzyme regulation epididymis fat exercise glucose blood level groups by age insulin blood level insulin sensitivity insulin tolerance test liver molecular mechanics nonhuman priority journal rat signal transduction swimming |
topic |
glucose glucose 6 phosphatase insulin insulin receptor insulin receptor beta insulin receptor substrate 1 protein tyrosine phosphatase 1B unclassified drug aging animal experiment controlled study diet restriction enzyme blood level enzyme regulation epididymis fat exercise glucose blood level groups by age insulin blood level insulin sensitivity insulin tolerance test liver molecular mechanics nonhuman priority journal rat signal transduction swimming |
description |
It is now commonly accepted that chronic inflammation associated with obesity during aging induces insulin resistance in the liver. In the present study, we investigated whether the improvement in insulin sensitivity and insulin signaling, mediated by acute exercise, could be associated with modulation of protein-tyrosine phosphatase 1B (PTP-1B) in the liver of old rats. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45 min rest period. Sixteen hours after the exercise, the rats were sacrificed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the fat mass was increased in old rats. The reduction in glucose disappearance rate (Kitt) observed in aged rats was restored 16 h after exercise. Aging increased the content of PTP-1B and attenuated insulin signaling in the liver of rats, a phenomenon that was reversed by exercise. Aging rats also increased the IRβ/PTP-1B and IRS-1/PTP-1B association in the liver when compared with young rats. Conversely, in the liver of exercised old rats, IRβ/PTP-1B and IRS-1/PTP-1B association was markedly decreased. Moreover, in the hepatic tissue of old rats, the insulin signalling was decreased and PEPCK and G6Pase levels were increased when compared with young rats. Interestingly, 16 h after acute exercise, the PEPCK and G6Pase protein level were decreased in the old exercised group. These results provide new insights into the mechanisms by which exercise restores insulin signalling in liver during aging. © 2013 Moura et al; licensee BioMed Central Ltd. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-25 2014-05-27T11:28:33Z 2014-05-27T11:28:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1742-4933-10-8 Immunity and Ageing, v. 10, n. 1, 2013. 1742-4933 http://hdl.handle.net/11449/74628 10.1186/1742-4933-10-8 WOS:000327218400001 2-s2.0-84874150350 2-s2.0-84874150350.pdf |
url |
http://dx.doi.org/10.1186/1742-4933-10-8 http://hdl.handle.net/11449/74628 |
identifier_str_mv |
Immunity and Ageing, v. 10, n. 1, 2013. 1742-4933 10.1186/1742-4933-10-8 WOS:000327218400001 2-s2.0-84874150350 2-s2.0-84874150350.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Immunity and Ageing 4.019 1,218 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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