Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fpls.2021.635784 http://hdl.handle.net/11449/229071 |
Resumo: | Flowering is of utmost relevance for the agricultural productivity of the sugarcane bioeconomy, but data and knowledge of the genetic mechanisms underlying its photoperiodic induction are still scarce. An understanding of the molecular mechanisms that regulate the transition from vegetative to reproductive growth in sugarcane could provide better control of flowering for breeding. This study aimed to investigate the transcriptome of +1 mature leaves of a sugarcane cultivar subjected to florally inductive and non-inductive photoperiodic treatments to identify gene expression patterns and molecular regulatory modules. We identified 7,083 differentially expressed (DE) genes, of which 5,623 showed significant identity to other plant genes. Functional group analysis showed differential regulation of important metabolic pathways involved in plant development, such as plant hormones (i.e., cytokinin, gibberellin, and abscisic acid), light reactions, and photorespiration. Gene ontology enrichment analysis revealed evidence of upregulated processes and functions related to the response to abiotic stress, photoprotection, photosynthesis, light harvesting, and pigment biosynthesis, whereas important categories related to growth and vegetative development of plants, such as plant organ morphogenesis, shoot system development, macromolecule metabolic process, and lignin biosynthesis, were downregulated. Also, out of 76 sugarcane transcripts considered putative orthologs to flowering genes from other plants (such as Arabidopsis thaliana, Oryza sativa, and Sorghum bicolor), 21 transcripts were DE. Nine DE genes related to flowering and response to photoperiod were analyzed either at mature or spindle leaves at two development stages corresponding to the early stage of induction and inflorescence primordia formation. Finally, we report a set of flowering-induced long non-coding RNAs and describe their level of conservation to other crops, many of which showed expression patterns correlated against those in the functionally grouped gene network. |
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Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditionsartificial inductionfloweringphotoperiodismsugarcanetranscriptomeFlowering is of utmost relevance for the agricultural productivity of the sugarcane bioeconomy, but data and knowledge of the genetic mechanisms underlying its photoperiodic induction are still scarce. An understanding of the molecular mechanisms that regulate the transition from vegetative to reproductive growth in sugarcane could provide better control of flowering for breeding. This study aimed to investigate the transcriptome of +1 mature leaves of a sugarcane cultivar subjected to florally inductive and non-inductive photoperiodic treatments to identify gene expression patterns and molecular regulatory modules. We identified 7,083 differentially expressed (DE) genes, of which 5,623 showed significant identity to other plant genes. Functional group analysis showed differential regulation of important metabolic pathways involved in plant development, such as plant hormones (i.e., cytokinin, gibberellin, and abscisic acid), light reactions, and photorespiration. Gene ontology enrichment analysis revealed evidence of upregulated processes and functions related to the response to abiotic stress, photoprotection, photosynthesis, light harvesting, and pigment biosynthesis, whereas important categories related to growth and vegetative development of plants, such as plant organ morphogenesis, shoot system development, macromolecule metabolic process, and lignin biosynthesis, were downregulated. Also, out of 76 sugarcane transcripts considered putative orthologs to flowering genes from other plants (such as Arabidopsis thaliana, Oryza sativa, and Sorghum bicolor), 21 transcripts were DE. Nine DE genes related to flowering and response to photoperiod were analyzed either at mature or spindle leaves at two development stages corresponding to the early stage of induction and inflorescence primordia formation. Finally, we report a set of flowering-induced long non-coding RNAs and describe their level of conservation to other crops, many of which showed expression patterns correlated against those in the functionally grouped gene network.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Biologia de Sistemas Departamento de Genética Evolução Microbiologia e Imunologia Instituto de Biologia Universidade Estadual de Campinas (UNICAMP)Departamento de Genética e Melhoramento de Plantas Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual de São Paulo (UNESP)Laboratório de Genômica de Plantas e Bioenergia (PGEMBL) Departamento de Biotecnologia Escola de Engenharia de Lorena (EEL) Universidade de São Paulo (USP)Instituto de Ciência e Tecnologia Universidade Federal de São Paulo (UNIFESP)Centro de Cana Instituto Agronômico de Campinas (IAC)School of Life Sciences The University of WarwickDepartamento de Genética e Melhoramento de Plantas Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual de São Paulo (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Instituto Agronômico de Campinas (IAC)The University of WarwickManechini, João Ricardo VieiraSantos, Paulo Henrique da Silva [UNESP]Romanel, ElissonBrito, Michael dos SantosScarpari, Maximiliano SallesJackson, StephenPinto, Luciana Rossini [UNESP]Vicentini, Renato2022-04-29T08:30:14Z2022-04-29T08:30:14Z2021-06-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fpls.2021.635784Frontiers in Plant Science, v. 12.1664-462Xhttp://hdl.handle.net/11449/22907110.3389/fpls.2021.6357842-s2.0-85109041766Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Plant Scienceinfo:eu-repo/semantics/openAccess2024-06-07T13:56:42Zoai:repositorio.unesp.br:11449/229071Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:43:47.894285Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions |
title |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions |
spellingShingle |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions Manechini, João Ricardo Vieira artificial induction flowering photoperiodism sugarcane transcriptome |
title_short |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions |
title_full |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions |
title_fullStr |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions |
title_full_unstemmed |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions |
title_sort |
Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions |
author |
Manechini, João Ricardo Vieira |
author_facet |
Manechini, João Ricardo Vieira Santos, Paulo Henrique da Silva [UNESP] Romanel, Elisson Brito, Michael dos Santos Scarpari, Maximiliano Salles Jackson, Stephen Pinto, Luciana Rossini [UNESP] Vicentini, Renato |
author_role |
author |
author2 |
Santos, Paulo Henrique da Silva [UNESP] Romanel, Elisson Brito, Michael dos Santos Scarpari, Maximiliano Salles Jackson, Stephen Pinto, Luciana Rossini [UNESP] Vicentini, Renato |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Instituto Agronômico de Campinas (IAC) The University of Warwick |
dc.contributor.author.fl_str_mv |
Manechini, João Ricardo Vieira Santos, Paulo Henrique da Silva [UNESP] Romanel, Elisson Brito, Michael dos Santos Scarpari, Maximiliano Salles Jackson, Stephen Pinto, Luciana Rossini [UNESP] Vicentini, Renato |
dc.subject.por.fl_str_mv |
artificial induction flowering photoperiodism sugarcane transcriptome |
topic |
artificial induction flowering photoperiodism sugarcane transcriptome |
description |
Flowering is of utmost relevance for the agricultural productivity of the sugarcane bioeconomy, but data and knowledge of the genetic mechanisms underlying its photoperiodic induction are still scarce. An understanding of the molecular mechanisms that regulate the transition from vegetative to reproductive growth in sugarcane could provide better control of flowering for breeding. This study aimed to investigate the transcriptome of +1 mature leaves of a sugarcane cultivar subjected to florally inductive and non-inductive photoperiodic treatments to identify gene expression patterns and molecular regulatory modules. We identified 7,083 differentially expressed (DE) genes, of which 5,623 showed significant identity to other plant genes. Functional group analysis showed differential regulation of important metabolic pathways involved in plant development, such as plant hormones (i.e., cytokinin, gibberellin, and abscisic acid), light reactions, and photorespiration. Gene ontology enrichment analysis revealed evidence of upregulated processes and functions related to the response to abiotic stress, photoprotection, photosynthesis, light harvesting, and pigment biosynthesis, whereas important categories related to growth and vegetative development of plants, such as plant organ morphogenesis, shoot system development, macromolecule metabolic process, and lignin biosynthesis, were downregulated. Also, out of 76 sugarcane transcripts considered putative orthologs to flowering genes from other plants (such as Arabidopsis thaliana, Oryza sativa, and Sorghum bicolor), 21 transcripts were DE. Nine DE genes related to flowering and response to photoperiod were analyzed either at mature or spindle leaves at two development stages corresponding to the early stage of induction and inflorescence primordia formation. Finally, we report a set of flowering-induced long non-coding RNAs and describe their level of conservation to other crops, many of which showed expression patterns correlated against those in the functionally grouped gene network. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-15 2022-04-29T08:30:14Z 2022-04-29T08:30:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fpls.2021.635784 Frontiers in Plant Science, v. 12. 1664-462X http://hdl.handle.net/11449/229071 10.3389/fpls.2021.635784 2-s2.0-85109041766 |
url |
http://dx.doi.org/10.3389/fpls.2021.635784 http://hdl.handle.net/11449/229071 |
identifier_str_mv |
Frontiers in Plant Science, v. 12. 1664-462X 10.3389/fpls.2021.635784 2-s2.0-85109041766 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Plant Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129239265837056 |