Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.4322/rbpv.01902006 http://hdl.handle.net/11449/3051 |
Resumo: | The aim of this study was to compare the detection of Ehrlichta cams morulae and DNA by nPCR in whole blood and spleen aspiration The sample Included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears E cants DNA was detected in 29/40 blood samples We verified that morulae detection is more efficient in cytological preparations from spleen aspiration on the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis |
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Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samplesDetecção de mórulas e DNA de Ehrlichia canis em sangue e em aspirado de baço em cães naturalmente infectadosEhrlichia canismorulaeSpleennPCR dogsThe aim of this study was to compare the detection of Ehrlichta cams morulae and DNA by nPCR in whole blood and spleen aspiration The sample Included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears E cants DNA was detected in 29/40 blood samples We verified that morulae detection is more efficient in cytological preparations from spleen aspiration on the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosisO objetivo desse estudo foi comparar a pesquisa de mórulas de Ehrlichia canis e a nPCR em sangue total e em aspirado de baço. Selecionaram-se 40 cães apresentando trombocitopenia associada a sinais e sintomas sugestivos de erliquiose canina. A pesquisa de mórula mostrou que dentre 35 amostras, 17 apresentaram mórulas nas preparações do baço, e duas nos esfregaços feitos a partir da papa leucocitária. O DNA de Ehrlichia canis foi detectado em 29 de 40 amostras de baço e em 30 de 40 no sangue. No presente estudo observou-se que a pesquisa de mórula é mais eficiente nas preparações citológicas obtidas da punção aspirativa do baço e que tanto a nPCR de baço quanto a de sangue foram eficientes no diagnóstico da doença.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, FCAV, Dept Clin & Cirurg Vet, BR-14884900 Jaboticabal, SP, BrazilUniv Uberaba UNIUBE, Uberaba, MG, BrazilUniv Estadual Paulista, FCAV, Med Vet Programa Posgrad, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, FCAV, Dept Patol Vet, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, FCAV, Dept Clin & Cirurg Vet, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, FCAV, Med Vet Programa Posgrad, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, FCAV, Dept Patol Vet, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 04/04383-2Brazilian Coll Veterinary ParasitologyUniversidade Estadual Paulista (Unesp)Univ Uberaba UNIUBEMaia Faria, Joice Lara [UNESP]Dagnone, Ana SilviaMunhoz, Thiago Demarchi [UNESP]Joao, Carolina Franchi [UNESP]Biscola Pereira, Wanderson AdrianoMachado, Rosangela Zacarias [UNESP]Tinucci-Costa, Mirela [UNESP]2014-05-20T13:16:05Z2014-05-20T13:16:05Z2010-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article98-102application/pdfhttp://dx.doi.org/10.4322/rbpv.01902006Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 19, n. 2, p. 98-102, 2010.0103-846Xhttp://hdl.handle.net/11449/3051S1984-29612010000200006WOS:000284796400006S1984-29612010000200006-en.pdf3254990612451836Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterináriainfo:eu-repo/semantics/openAccess2023-11-30T06:20:13Zoai:repositorio.unesp.br:11449/3051Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-30T06:20:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples Detecção de mórulas e DNA de Ehrlichia canis em sangue e em aspirado de baço em cães naturalmente infectados |
| title |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples |
| spellingShingle |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples Maia Faria, Joice Lara [UNESP] Ehrlichia canis morulae Spleen nPCR dogs |
| title_short |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples |
| title_full |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples |
| title_fullStr |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples |
| title_full_unstemmed |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples |
| title_sort |
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples |
| author |
Maia Faria, Joice Lara [UNESP] |
| author_facet |
Maia Faria, Joice Lara [UNESP] Dagnone, Ana Silvia Munhoz, Thiago Demarchi [UNESP] Joao, Carolina Franchi [UNESP] Biscola Pereira, Wanderson Adriano Machado, Rosangela Zacarias [UNESP] Tinucci-Costa, Mirela [UNESP] |
| author_role |
author |
| author2 |
Dagnone, Ana Silvia Munhoz, Thiago Demarchi [UNESP] Joao, Carolina Franchi [UNESP] Biscola Pereira, Wanderson Adriano Machado, Rosangela Zacarias [UNESP] Tinucci-Costa, Mirela [UNESP] |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Uberaba UNIUBE |
| dc.contributor.author.fl_str_mv |
Maia Faria, Joice Lara [UNESP] Dagnone, Ana Silvia Munhoz, Thiago Demarchi [UNESP] Joao, Carolina Franchi [UNESP] Biscola Pereira, Wanderson Adriano Machado, Rosangela Zacarias [UNESP] Tinucci-Costa, Mirela [UNESP] |
| dc.subject.por.fl_str_mv |
Ehrlichia canis morulae Spleen nPCR dogs |
| topic |
Ehrlichia canis morulae Spleen nPCR dogs |
| description |
The aim of this study was to compare the detection of Ehrlichta cams morulae and DNA by nPCR in whole blood and spleen aspiration The sample Included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears E cants DNA was detected in 29/40 blood samples We verified that morulae detection is more efficient in cytological preparations from spleen aspiration on the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010-04-01 2014-05-20T13:16:05Z 2014-05-20T13:16:05Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4322/rbpv.01902006 Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 19, n. 2, p. 98-102, 2010. 0103-846X http://hdl.handle.net/11449/3051 S1984-29612010000200006 WOS:000284796400006 S1984-29612010000200006-en.pdf 3254990612451836 |
| url |
http://dx.doi.org/10.4322/rbpv.01902006 http://hdl.handle.net/11449/3051 |
| identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 19, n. 2, p. 98-102, 2010. 0103-846X S1984-29612010000200006 WOS:000284796400006 S1984-29612010000200006-en.pdf 3254990612451836 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária |
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info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
98-102 application/pdf |
| dc.publisher.none.fl_str_mv |
Brazilian Coll Veterinary Parasitology |
| publisher.none.fl_str_mv |
Brazilian Coll Veterinary Parasitology |
| dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1799965125620269056 |