Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/97902 |
Resumo: | This project has as main objective the development of nanowires for application in biosensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they haver virtually the same dimensions as the molecules that should detect, these nanosensors could revolutionize the diagnoses way are made in medical and biological examinations. Therefores, in this research, we prepared and characterized multithreaded nanowires usind different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0,4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0,4 V. The analyitcal curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use of nanowires for the preparation of biosensors becomes and alternative to direct detection of glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential less than 0.4 V. The analytical curve for the determination o glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor... |
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Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturadosBiossensoresGlicoseMateriais nanoestruturadosBiosensorsThis project has as main objective the development of nanowires for application in biosensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they haver virtually the same dimensions as the molecules that should detect, these nanosensors could revolutionize the diagnoses way are made in medical and biological examinations. Therefores, in this research, we prepared and characterized multithreaded nanowires usind different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0,4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0,4 V. The analyitcal curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use of nanowires for the preparation of biosensors becomes and alternative to direct detection of glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential less than 0.4 V. The analytical curve for the determination o glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor...This project has as main objective the development of nanowires for application in bionsensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they have virtually the same dimensions as the molecules that should detect, these nanosensors could revolutioneze the diagnoses way are made in medical and biological examinations. Therefore, in this research, we prepared and characterized multithreaded nanowires using different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the bionsensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0.4 V. The analytical curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use... (Complete abstract click electronic access below)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 12/01402-2Universidade Estadual Paulista (Unesp)Pedrosa, Valber de Albuquerque [UNESP]Universidade Estadual Paulista (Unesp)Gerola, Gislaine Passarella [UNESP]2014-06-11T19:29:09Z2014-06-11T19:29:09Z2014-01-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis63 f. : il.application/pdfGEROLA, Gislaine Passarella. Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados. 2014. 63 f. , 2014.http://hdl.handle.net/11449/97902000734760gerola_gp_me_bauru.pdf33004056083P7Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-10-05T06:09:06Zoai:repositorio.unesp.br:11449/97902Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:06:55.273761Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados |
title |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados |
spellingShingle |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados Gerola, Gislaine Passarella [UNESP] Biossensores Glicose Materiais nanoestruturados Biosensors |
title_short |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados |
title_full |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados |
title_fullStr |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados |
title_full_unstemmed |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados |
title_sort |
Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados |
author |
Gerola, Gislaine Passarella [UNESP] |
author_facet |
Gerola, Gislaine Passarella [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pedrosa, Valber de Albuquerque [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gerola, Gislaine Passarella [UNESP] |
dc.subject.por.fl_str_mv |
Biossensores Glicose Materiais nanoestruturados Biosensors |
topic |
Biossensores Glicose Materiais nanoestruturados Biosensors |
description |
This project has as main objective the development of nanowires for application in biosensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they haver virtually the same dimensions as the molecules that should detect, these nanosensors could revolutionize the diagnoses way are made in medical and biological examinations. Therefores, in this research, we prepared and characterized multithreaded nanowires usind different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0,4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0,4 V. The analyitcal curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use of nanowires for the preparation of biosensors becomes and alternative to direct detection of glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential less than 0.4 V. The analytical curve for the determination o glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor... |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-11T19:29:09Z 2014-06-11T19:29:09Z 2014-01-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GEROLA, Gislaine Passarella. Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados. 2014. 63 f. , 2014. http://hdl.handle.net/11449/97902 000734760 gerola_gp_me_bauru.pdf 33004056083P7 |
identifier_str_mv |
GEROLA, Gislaine Passarella. Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados. 2014. 63 f. , 2014. 000734760 gerola_gp_me_bauru.pdf 33004056083P7 |
url |
http://hdl.handle.net/11449/97902 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
63 f. : il. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128317343137792 |