Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados

Detalhes bibliográficos
Autor(a) principal: Gerola, Gislaine Passarella [UNESP]
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/97902
Resumo: This project has as main objective the development of nanowires for application in biosensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they haver virtually the same dimensions as the molecules that should detect, these nanosensors could revolutionize the diagnoses way are made in medical and biological examinations. Therefores, in this research, we prepared and characterized multithreaded nanowires usind different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0,4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0,4 V. The analyitcal curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use of nanowires for the preparation of biosensors becomes and alternative to direct detection of glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential less than 0.4 V. The analytical curve for the determination o glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor...
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spelling Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturadosBiossensoresGlicoseMateriais nanoestruturadosBiosensorsThis project has as main objective the development of nanowires for application in biosensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they haver virtually the same dimensions as the molecules that should detect, these nanosensors could revolutionize the diagnoses way are made in medical and biological examinations. Therefores, in this research, we prepared and characterized multithreaded nanowires usind different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0,4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0,4 V. The analyitcal curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use of nanowires for the preparation of biosensors becomes and alternative to direct detection of glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential less than 0.4 V. The analytical curve for the determination o glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor...This project has as main objective the development of nanowires for application in bionsensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they have virtually the same dimensions as the molecules that should detect, these nanosensors could revolutioneze the diagnoses way are made in medical and biological examinations. Therefore, in this research, we prepared and characterized multithreaded nanowires using different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the bionsensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0.4 V. The analytical curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use... (Complete abstract click electronic access below)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 12/01402-2Universidade Estadual Paulista (Unesp)Pedrosa, Valber de Albuquerque [UNESP]Universidade Estadual Paulista (Unesp)Gerola, Gislaine Passarella [UNESP]2014-06-11T19:29:09Z2014-06-11T19:29:09Z2014-01-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis63 f. : il.application/pdfGEROLA, Gislaine Passarella. Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados. 2014. 63 f. , 2014.http://hdl.handle.net/11449/97902000734760gerola_gp_me_bauru.pdf33004056083P7Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-10-05T06:09:06Zoai:repositorio.unesp.br:11449/97902Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:06:55.273761Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
title Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
spellingShingle Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
Gerola, Gislaine Passarella [UNESP]
Biossensores
Glicose
Materiais nanoestruturados
Biosensors
title_short Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
title_full Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
title_fullStr Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
title_full_unstemmed Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
title_sort Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados
author Gerola, Gislaine Passarella [UNESP]
author_facet Gerola, Gislaine Passarella [UNESP]
author_role author
dc.contributor.none.fl_str_mv Pedrosa, Valber de Albuquerque [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Gerola, Gislaine Passarella [UNESP]
dc.subject.por.fl_str_mv Biossensores
Glicose
Materiais nanoestruturados
Biosensors
topic Biossensores
Glicose
Materiais nanoestruturados
Biosensors
description This project has as main objective the development of nanowires for application in biosensors. Nanomaterials have been highlighted by high sensitivity when applied to biological sensors. As they haver virtually the same dimensions as the molecules that should detect, these nanosensors could revolutionize the diagnoses way are made in medical and biological examinations. Therefores, in this research, we prepared and characterized multithreaded nanowires usind different types of metal and polymer. These nanowires were prepared by electrochemical deposition process and characterized by scanning electron microscopy (SEM). The synthesized nanowires have been changed for the attachment of the enzyme glucose oxidase. Several experimental parameters of biosensor was evaluated as the optimization of pH (at 6), as a function of the peak current obtained from glucose; the enzyme concentration of 0,4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential variation, where it was observed that the ideal is to use potential less than 0,4 V. The analyitcal curve for the determination of glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor determined was 3.7 x 10-7 mol L-1. The biosensor showed high selectivity only for detection of glucose and maintained a good response to glucose sensing (85%) in 30 days. Thus, the use of nanowires for the preparation of biosensors becomes and alternative to direct detection of glucose; the enzyme concentration of 0.4 mg ml-1 required to prepare the biosensor; as well as the study of electrochemical potential less than 0.4 V. The analytical curve for the determination o glucose showed a slope of 7.95 uA mol-1, with a correlation coefficient of 0.997 and detection limit of the biosensor...
publishDate 2014
dc.date.none.fl_str_mv 2014-06-11T19:29:09Z
2014-06-11T19:29:09Z
2014-01-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv GEROLA, Gislaine Passarella. Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados. 2014. 63 f. , 2014.
http://hdl.handle.net/11449/97902
000734760
gerola_gp_me_bauru.pdf
33004056083P7
identifier_str_mv GEROLA, Gislaine Passarella. Utilização da biointerface nanofios/enzimas para o desenvolvimento de biossensores nanoestruturados. 2014. 63 f. , 2014.
000734760
gerola_gp_me_bauru.pdf
33004056083P7
url http://hdl.handle.net/11449/97902
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 63 f. : il.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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