Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis

Detalhes bibliográficos
Autor(a) principal: Veriato, Thaís S.
Data de Publicação: 2023
Outros Autores: Fontoura, Inglid, Oliveira, Luciane D. [UNESP], Raniero, Leandro J., Castilho, Maiara L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s43440-023-00491-3
http://hdl.handle.net/11449/247379
Resumo: Background: Bacterial resistance is defined as a microorganism’s capacity to develop mechanisms for resisting a determined antimicrobial. Gram-positive bacteria, such as Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), are internationally recognized among the isolates with this resistance profile. In this context, the demand for new medicines has risen, and silver nanoparticles (AgNPs) have been highlighted, especially for their anti-bacterial effects. To develop a nano-antibiotic for treating these Gram-positive strains, we herein report synthesizing and characterizing a nano-antibiotic based on AgNPs functionalized with the complex vancomycin–cysteamine. Methods: AgNPs were produced using the bottom-up methodology and functionalized with vancomycin modified by the carbodiimide chemistry, forming Ag@vancomycin. Susceptibility tests were performed using S. aureus and E. faecalis strains to assess the bacteriostatic and bactericidal potential of the developed nano-antibiotic. Results: Fourier transform infrared spectroscopy measurements showed the efficacy of vancomycin chemical modification, and the characteristic bands of AgNPs functionalization with the antibiotic. The increase in the nano-antibiotic average hydrodynamic diameter observed by dynamic light scattering proved the presence of vancomycin at the surface of AgNPs. The data from the minimum inhibitory concentration and minimal bactericidal concentration assays tested on standard and clinical planktonic strains of S. aureus and E. faecalis presented excellent performance. Conclusion: The results indicate the promising development of a new nano-antibiotic in which the functionalization potentiates the bacteriostatic action of AgNPs and vancomycin with greater efficacy against Gram-positive strains.
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spelling Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalisEnterococcus faecalisSilver nanoparticlesStaphylococcus aureusVancomycinBackground: Bacterial resistance is defined as a microorganism’s capacity to develop mechanisms for resisting a determined antimicrobial. Gram-positive bacteria, such as Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), are internationally recognized among the isolates with this resistance profile. In this context, the demand for new medicines has risen, and silver nanoparticles (AgNPs) have been highlighted, especially for their anti-bacterial effects. To develop a nano-antibiotic for treating these Gram-positive strains, we herein report synthesizing and characterizing a nano-antibiotic based on AgNPs functionalized with the complex vancomycin–cysteamine. Methods: AgNPs were produced using the bottom-up methodology and functionalized with vancomycin modified by the carbodiimide chemistry, forming Ag@vancomycin. Susceptibility tests were performed using S. aureus and E. faecalis strains to assess the bacteriostatic and bactericidal potential of the developed nano-antibiotic. Results: Fourier transform infrared spectroscopy measurements showed the efficacy of vancomycin chemical modification, and the characteristic bands of AgNPs functionalization with the antibiotic. The increase in the nano-antibiotic average hydrodynamic diameter observed by dynamic light scattering proved the presence of vancomycin at the surface of AgNPs. The data from the minimum inhibitory concentration and minimal bactericidal concentration assays tested on standard and clinical planktonic strains of S. aureus and E. faecalis presented excellent performance. Conclusion: The results indicate the promising development of a new nano-antibiotic in which the functionalization potentiates the bacteriostatic action of AgNPs and vancomycin with greater efficacy against Gram-positive strains.Bionanotechnology Laboratory Research & Development Institute University of Vale do Paraíba, São PauloDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology Sao Paulo State University, São PauloNanosensors Laboratory Research & Development Institute University of Vale do Paraíba, São PauloDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology Sao Paulo State University, São PauloUniversity of Vale do ParaíbaUniversidade Estadual Paulista (UNESP)Veriato, Thaís S.Fontoura, InglidOliveira, Luciane D. [UNESP]Raniero, Leandro J.Castilho, Maiara L.2023-07-29T13:14:31Z2023-07-29T13:14:31Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s43440-023-00491-3Pharmacological Reports.2299-56841734-1140http://hdl.handle.net/11449/24737910.1007/s43440-023-00491-32-s2.0-85159322240Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmacological Reportsinfo:eu-repo/semantics/openAccess2023-07-29T13:14:31Zoai:repositorio.unesp.br:11449/247379Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:37:27.516129Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
spellingShingle Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
Veriato, Thaís S.
Enterococcus faecalis
Silver nanoparticles
Staphylococcus aureus
Vancomycin
title_short Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_full Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_fullStr Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_full_unstemmed Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
title_sort Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin–cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis
author Veriato, Thaís S.
author_facet Veriato, Thaís S.
Fontoura, Inglid
Oliveira, Luciane D. [UNESP]
Raniero, Leandro J.
Castilho, Maiara L.
author_role author
author2 Fontoura, Inglid
Oliveira, Luciane D. [UNESP]
Raniero, Leandro J.
Castilho, Maiara L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv University of Vale do Paraíba
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Veriato, Thaís S.
Fontoura, Inglid
Oliveira, Luciane D. [UNESP]
Raniero, Leandro J.
Castilho, Maiara L.
dc.subject.por.fl_str_mv Enterococcus faecalis
Silver nanoparticles
Staphylococcus aureus
Vancomycin
topic Enterococcus faecalis
Silver nanoparticles
Staphylococcus aureus
Vancomycin
description Background: Bacterial resistance is defined as a microorganism’s capacity to develop mechanisms for resisting a determined antimicrobial. Gram-positive bacteria, such as Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), are internationally recognized among the isolates with this resistance profile. In this context, the demand for new medicines has risen, and silver nanoparticles (AgNPs) have been highlighted, especially for their anti-bacterial effects. To develop a nano-antibiotic for treating these Gram-positive strains, we herein report synthesizing and characterizing a nano-antibiotic based on AgNPs functionalized with the complex vancomycin–cysteamine. Methods: AgNPs were produced using the bottom-up methodology and functionalized with vancomycin modified by the carbodiimide chemistry, forming Ag@vancomycin. Susceptibility tests were performed using S. aureus and E. faecalis strains to assess the bacteriostatic and bactericidal potential of the developed nano-antibiotic. Results: Fourier transform infrared spectroscopy measurements showed the efficacy of vancomycin chemical modification, and the characteristic bands of AgNPs functionalization with the antibiotic. The increase in the nano-antibiotic average hydrodynamic diameter observed by dynamic light scattering proved the presence of vancomycin at the surface of AgNPs. The data from the minimum inhibitory concentration and minimal bactericidal concentration assays tested on standard and clinical planktonic strains of S. aureus and E. faecalis presented excellent performance. Conclusion: The results indicate the promising development of a new nano-antibiotic in which the functionalization potentiates the bacteriostatic action of AgNPs and vancomycin with greater efficacy against Gram-positive strains.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:14:31Z
2023-07-29T13:14:31Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s43440-023-00491-3
Pharmacological Reports.
2299-5684
1734-1140
http://hdl.handle.net/11449/247379
10.1007/s43440-023-00491-3
2-s2.0-85159322240
url http://dx.doi.org/10.1007/s43440-023-00491-3
http://hdl.handle.net/11449/247379
identifier_str_mv Pharmacological Reports.
2299-5684
1734-1140
10.1007/s43440-023-00491-3
2-s2.0-85159322240
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pharmacological Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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