DNA damage in canine transmissible venereal tumor cells

Detalhes bibliográficos
Autor(a) principal: Amaral, Anne Santos do
Data de Publicação: 2011
Outros Autores: Ferreira, Isabelle [UNESP], Colodel, Márcia Moleta [UNESP], Salvadori, Daisy Maria Favero [UNESP], Rocha, Noeme Sousa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://revistas.ulusofona.pt/index.php/rlcmv/article/view/2388
http://hdl.handle.net/11449/140566
Resumo: The Transmissible Venereal Tumor (TVT) has been classified according to the predominant cell type as follows: lymphocytoid, plasmocytoid, and mixed. Various degrees of aggressiveness with large array of biological behaviour have been described according to the TVT cell lineages, the present study was designed to investigate the level of DNA damage in the three TVT cell types aiming a better understanding of mechanisms related to the aggressiveness of this neoplasia. A total of 35 dogs were evaluated, with no restriction regarding sex, age or breed, and with clinical and cytological diagnoses for TVT. Cell samples from the 35 tumoral neoplasmas were obtained by fine needle aspiration and taken to cytology and DNA damage analysis by the comet assay. From the 35 TVT cases, 12 (34.3%) were plasmocytoid, 11 (31.4%) lymphocytoid, and 12 (34.3%) mixed. Statistically significant (p<0.05) higher level of DNA damage was detected in lymphocytoid TVT cells when compared to the other two types.
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spelling DNA damage in canine transmissible venereal tumor cellsThe Transmissible Venereal Tumor (TVT) has been classified according to the predominant cell type as follows: lymphocytoid, plasmocytoid, and mixed. Various degrees of aggressiveness with large array of biological behaviour have been described according to the TVT cell lineages, the present study was designed to investigate the level of DNA damage in the three TVT cell types aiming a better understanding of mechanisms related to the aggressiveness of this neoplasia. A total of 35 dogs were evaluated, with no restriction regarding sex, age or breed, and with clinical and cytological diagnoses for TVT. Cell samples from the 35 tumoral neoplasmas were obtained by fine needle aspiration and taken to cytology and DNA damage analysis by the comet assay. From the 35 TVT cases, 12 (34.3%) were plasmocytoid, 11 (31.4%) lymphocytoid, and 12 (34.3%) mixed. Statistically significant (p<0.05) higher level of DNA damage was detected in lymphocytoid TVT cells when compared to the other two types.O Tumor Venéreo Transmissível (TVT) tem sido classificado de acordo com o tipo celular predominante da seguinte forma: linfocitóide, plasmocitóide e misto. Vários graus de agressividade com grande variedade de comportamento biológico, têm sido descritos de acordo com as morfologias das células do TVT. O presente estudo teve como objectivo investigar o nível de danos no DNA nos três tipos de células do TVT, visando uma melhor compreensão dos mecanismos relacionados com a agressividade dessa neoplasia. Um total de 35 cães, sem restrição quanto à idade, sexo ou raça, e com diagnóstico clínico e citológico de TVT foram avaliados. Amostras de células foram obtidas a partir de 35 tumores, por aspiração com agulha fina, e realizada citologia e análise dos danos no DNA, pelo Ensaio Cometa. Dos 35 casos de TVT, 12 (34,3%) foram plasmocitóide, 11 (31,4%) linfocitóide, e 12 (34,3%) misto. Estatísticamente (p <0,05) o TVT linfocitóide apresentou maior nível de danos no DNA quando comparado com os outros dois tipos.Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina (FMB), Departamento de Patologia, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Departamento de Clínica Veterinária, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina (FMB), Departamento de Patologia, Botucatu, SP, BrasilUniversidade Federal de Santa Maria (UFSM)Universidade Estadual Paulista (Unesp)Amaral, Anne Santos doFerreira, Isabelle [UNESP]Colodel, Márcia Moleta [UNESP]Salvadori, Daisy Maria Favero [UNESP]Rocha, Noeme Sousa [UNESP]2016-07-07T12:34:28Z2016-07-07T12:34:28Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-5application/pdfhttp://revistas.ulusofona.pt/index.php/rlcmv/article/view/2388Revista Lusófona de Ciência e Medicina Veterinária, v. 4, p. 1-5, 2011.1646-8805http://hdl.handle.net/11449/140566ISSN1646-8805-2011-04-01-05.pdf505111875298090360777359184692840000-0002-8188-8149Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Lusófona de Ciência e Medicina Veterináriainfo:eu-repo/semantics/openAccess2024-09-03T13:14:41Zoai:repositorio.unesp.br:11449/140566Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv DNA damage in canine transmissible venereal tumor cells
title DNA damage in canine transmissible venereal tumor cells
spellingShingle DNA damage in canine transmissible venereal tumor cells
Amaral, Anne Santos do
title_short DNA damage in canine transmissible venereal tumor cells
title_full DNA damage in canine transmissible venereal tumor cells
title_fullStr DNA damage in canine transmissible venereal tumor cells
title_full_unstemmed DNA damage in canine transmissible venereal tumor cells
title_sort DNA damage in canine transmissible venereal tumor cells
author Amaral, Anne Santos do
author_facet Amaral, Anne Santos do
Ferreira, Isabelle [UNESP]
Colodel, Márcia Moleta [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
Rocha, Noeme Sousa [UNESP]
author_role author
author2 Ferreira, Isabelle [UNESP]
Colodel, Márcia Moleta [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
Rocha, Noeme Sousa [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Santa Maria (UFSM)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Amaral, Anne Santos do
Ferreira, Isabelle [UNESP]
Colodel, Márcia Moleta [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
Rocha, Noeme Sousa [UNESP]
description The Transmissible Venereal Tumor (TVT) has been classified according to the predominant cell type as follows: lymphocytoid, plasmocytoid, and mixed. Various degrees of aggressiveness with large array of biological behaviour have been described according to the TVT cell lineages, the present study was designed to investigate the level of DNA damage in the three TVT cell types aiming a better understanding of mechanisms related to the aggressiveness of this neoplasia. A total of 35 dogs were evaluated, with no restriction regarding sex, age or breed, and with clinical and cytological diagnoses for TVT. Cell samples from the 35 tumoral neoplasmas were obtained by fine needle aspiration and taken to cytology and DNA damage analysis by the comet assay. From the 35 TVT cases, 12 (34.3%) were plasmocytoid, 11 (31.4%) lymphocytoid, and 12 (34.3%) mixed. Statistically significant (p<0.05) higher level of DNA damage was detected in lymphocytoid TVT cells when compared to the other two types.
publishDate 2011
dc.date.none.fl_str_mv 2011
2016-07-07T12:34:28Z
2016-07-07T12:34:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://revistas.ulusofona.pt/index.php/rlcmv/article/view/2388
Revista Lusófona de Ciência e Medicina Veterinária, v. 4, p. 1-5, 2011.
1646-8805
http://hdl.handle.net/11449/140566
ISSN1646-8805-2011-04-01-05.pdf
5051118752980903
6077735918469284
0000-0002-8188-8149
url http://revistas.ulusofona.pt/index.php/rlcmv/article/view/2388
http://hdl.handle.net/11449/140566
identifier_str_mv Revista Lusófona de Ciência e Medicina Veterinária, v. 4, p. 1-5, 2011.
1646-8805
ISSN1646-8805-2011-04-01-05.pdf
5051118752980903
6077735918469284
0000-0002-8188-8149
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Lusófona de Ciência e Medicina Veterinária
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-5
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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