Enfermedad neurológica bovina con diagnóstico no conclusivo en la región norte de Colombia: análisis histológico e inmunohistoquímico

• Autor(a) principal: Montoya-Flórez, Luis Mauricio
• Data de Publicação: 2015
• Outros Autores: Ocampo, Pablo Eduardo, Rocha, Noeme Sousa [UNESP], Pedraza, Francisco Javier
• Tipo de documento: Artigo
• Idioma: spa
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.15446/rfmvz.v62n1.49384; http://hdl.handle.net/11449/140542
• topic: Immunofluorescence; Inmunohistochemistry; Lyssavirus; Meningoencephalitis; Inmunofluorescencia; Inmunohistoquímica; Lyssavirus; Meningoencefalitis

Bovine neurological disease generates high economic losses in herds and some of its etiological agents are zoonotic, situations that make priority their study. The aim of this study was to identify antigens rabies virus and bovine herpesvirus-5 (BoHV-5) in cattle brain samples archived as cases of neurological disease with no conclusive diagnosis. 10 samples were selected from laboratory of the Colombian Agricultural Institute –ICA– at Monteria. Tissues were analyzed by histopathology and immunohistochemistry techniques. For immunohistochemical analysis, three antibodies were used: two against antigens of rabies virus at a dilution of 1:200, and one against antigens of BoHV-5 at a dilution of 1:100. Histologically lesions i nine cases lesions that varied in type and severity were observed; antigens detection of rabies virus could be demonstrated by immunohistochemistry in 20% (2/10) of cases and could not be demonstrated in any case the presence BoHV-5. We discuss on the histopathological and immunohistochemical finding, it is concluded on the importance of perform association of the two techniques to assist the establishing of the neurological disease etiology, especially in case without diagnostic.