Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2013.09.019 http://hdl.handle.net/11449/219971 |
Resumo: | Ethnopharmacological relevance: The great potential of phytotherapic drugs for treating and preventing inflammatory diseases mediated by increased neutrophil reactive oxygen species (ROS) generation has guided the search for new natural products with antioxidant and immunomodulatory properties. Baccharis dracunculifolia D.C. (Asteraceae), the main botanical source of Brazilian green propolis, is a native plant from Brazil widely used in folk medicine as anti-inflammatory. This study aims: (a) to determine the influence of seasonality on the chemical profile and biological activity of Baccharis dracunculifolia (Asteraceae) leaf extracts (BdE); (b) to analyze the correlation between the major compounds and the ability of BdE to modulate the superoxide anion and total ROS generation by human neutrophils. Materials and methods: The extracts were obtained from leaf samples collected monthly during one year. The superoxide anion and total ROS generation were assessed by the lucigenin (CL-luc)- and luminol (CL-lum)-enhanced chemiluminescence assays. Results: Seasonality influenced more the quantitative than the qualitative chemical profile of B. dracunculifolia, and affected its biological activity. The major compounds identified were caffeic acid, p-coumaric acid, aromadendrin-4′- methyl ether (AME), isosakuranetin and artepillin C. The IC50 values obtained for CL-lum and CL-luc inhibition by BdE ranged from 8.1-15.8 and 5.8-13.3 μg mL-1, respectively, and correlated positively with caffeic acid concentration. CL-luc inhibition correlated negatively with the concentration of artepillin C, AME, isosakuranetin and total flavonoids. The BdE sample from May/07 inhibited CL-lum and CL-luc the most strongly (IC 50=8.1±1.6 and 5.8±1.0 μg mL-1, respectively), and contained the highest ratio of caffeic acid to the other isolated compounds; so, this ratio could be employed as chemical marker for this biological activity of B. dracunculifolia. Conclusion: The ability of B. dracunculifolia to inhibit the neutrophil ROS generation depends more on the type and ratio of phenolic compounds and flavonoids than on their high absolute concentrations. Together, our results help select the most appropriate plant material for the production of phytotherapic drugs to be used in the treatment of inflammatory diseases mediated by increased neutrophil activation. © 2013 Elsevier Ireland Ltd. All rights reserved. |
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Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compoundsAntioxidant activityBaccharis dracunculifoliaFlavonoidsNeutrophilsPhenolic compoundsSeasonalityEthnopharmacological relevance: The great potential of phytotherapic drugs for treating and preventing inflammatory diseases mediated by increased neutrophil reactive oxygen species (ROS) generation has guided the search for new natural products with antioxidant and immunomodulatory properties. Baccharis dracunculifolia D.C. (Asteraceae), the main botanical source of Brazilian green propolis, is a native plant from Brazil widely used in folk medicine as anti-inflammatory. This study aims: (a) to determine the influence of seasonality on the chemical profile and biological activity of Baccharis dracunculifolia (Asteraceae) leaf extracts (BdE); (b) to analyze the correlation between the major compounds and the ability of BdE to modulate the superoxide anion and total ROS generation by human neutrophils. Materials and methods: The extracts were obtained from leaf samples collected monthly during one year. The superoxide anion and total ROS generation were assessed by the lucigenin (CL-luc)- and luminol (CL-lum)-enhanced chemiluminescence assays. Results: Seasonality influenced more the quantitative than the qualitative chemical profile of B. dracunculifolia, and affected its biological activity. The major compounds identified were caffeic acid, p-coumaric acid, aromadendrin-4′- methyl ether (AME), isosakuranetin and artepillin C. The IC50 values obtained for CL-lum and CL-luc inhibition by BdE ranged from 8.1-15.8 and 5.8-13.3 μg mL-1, respectively, and correlated positively with caffeic acid concentration. CL-luc inhibition correlated negatively with the concentration of artepillin C, AME, isosakuranetin and total flavonoids. The BdE sample from May/07 inhibited CL-lum and CL-luc the most strongly (IC 50=8.1±1.6 and 5.8±1.0 μg mL-1, respectively), and contained the highest ratio of caffeic acid to the other isolated compounds; so, this ratio could be employed as chemical marker for this biological activity of B. dracunculifolia. Conclusion: The ability of B. dracunculifolia to inhibit the neutrophil ROS generation depends more on the type and ratio of phenolic compounds and flavonoids than on their high absolute concentrations. Together, our results help select the most appropriate plant material for the production of phytotherapic drugs to be used in the treatment of inflammatory diseases mediated by increased neutrophil activation. © 2013 Elsevier Ireland Ltd. All rights reserved.Departamento de Física e Química Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Avenida do Café s/n, Monte Alegre, 14040-903 Ribeirão Preto, SPLaboratório de Pesquisa Desenvolvimento and Inovação, Apis Flora Indl. Coml. Ltda.; Rua Triunfo 945, 14020-670 Ribeirão Preto, SPLaboratório de Farmacognosia Departamento de Ciências Farmacêuticas Universidade de São Paulo, Avenida do Café s/n, Monte Alegre, 14040-903 Ribeirão Preto, SPInstituto de Quimíca Universidade Estadual Paulista, Rua Francisco Degni n. 55, 14800-900 Araraquara, SPInstituto de Quimíca Universidade Estadual Paulista, Rua Francisco Degni n. 55, 14800-900 Araraquara, SPUniversidade de São Paulo (USP)Desenvolvimento and InovaçãoUniversidade Estadual Paulista (UNESP)Figueiredo-Rinhel, Andréa S.G.Kabeya, Luciana M.Bueno, Paula C.P. [UNESP]Jorge-Tiossi, Renata F.Azzolini, Ana Elisa C.S.Bastos, Jairo K.Lucisano-Valim, Yara Maria2022-04-28T18:58:51Z2022-04-28T18:58:51Z2013-11-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article655-664http://dx.doi.org/10.1016/j.jep.2013.09.019Journal of Ethnopharmacology, v. 150, n. 2, p. 655-664, 2013.0378-87411872-7573http://hdl.handle.net/11449/21997110.1016/j.jep.2013.09.0192-s2.0-84887450985Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacologyinfo:eu-repo/semantics/openAccess2022-04-28T18:58:51Zoai:repositorio.unesp.br:11449/219971Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T18:58:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds |
title |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds |
spellingShingle |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds Figueiredo-Rinhel, Andréa S.G. Antioxidant activity Baccharis dracunculifolia Flavonoids Neutrophils Phenolic compounds Seasonality |
title_short |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds |
title_full |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds |
title_fullStr |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds |
title_full_unstemmed |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds |
title_sort |
Inhibition of the human neutrophil oxidative metabolism by Baccharis dracunculifolia DC (Asteraceae) is influenced by seasonality and the ratio of caffeic acid to other phenolic compounds |
author |
Figueiredo-Rinhel, Andréa S.G. |
author_facet |
Figueiredo-Rinhel, Andréa S.G. Kabeya, Luciana M. Bueno, Paula C.P. [UNESP] Jorge-Tiossi, Renata F. Azzolini, Ana Elisa C.S. Bastos, Jairo K. Lucisano-Valim, Yara Maria |
author_role |
author |
author2 |
Kabeya, Luciana M. Bueno, Paula C.P. [UNESP] Jorge-Tiossi, Renata F. Azzolini, Ana Elisa C.S. Bastos, Jairo K. Lucisano-Valim, Yara Maria |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Desenvolvimento and Inovação Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Figueiredo-Rinhel, Andréa S.G. Kabeya, Luciana M. Bueno, Paula C.P. [UNESP] Jorge-Tiossi, Renata F. Azzolini, Ana Elisa C.S. Bastos, Jairo K. Lucisano-Valim, Yara Maria |
dc.subject.por.fl_str_mv |
Antioxidant activity Baccharis dracunculifolia Flavonoids Neutrophils Phenolic compounds Seasonality |
topic |
Antioxidant activity Baccharis dracunculifolia Flavonoids Neutrophils Phenolic compounds Seasonality |
description |
Ethnopharmacological relevance: The great potential of phytotherapic drugs for treating and preventing inflammatory diseases mediated by increased neutrophil reactive oxygen species (ROS) generation has guided the search for new natural products with antioxidant and immunomodulatory properties. Baccharis dracunculifolia D.C. (Asteraceae), the main botanical source of Brazilian green propolis, is a native plant from Brazil widely used in folk medicine as anti-inflammatory. This study aims: (a) to determine the influence of seasonality on the chemical profile and biological activity of Baccharis dracunculifolia (Asteraceae) leaf extracts (BdE); (b) to analyze the correlation between the major compounds and the ability of BdE to modulate the superoxide anion and total ROS generation by human neutrophils. Materials and methods: The extracts were obtained from leaf samples collected monthly during one year. The superoxide anion and total ROS generation were assessed by the lucigenin (CL-luc)- and luminol (CL-lum)-enhanced chemiluminescence assays. Results: Seasonality influenced more the quantitative than the qualitative chemical profile of B. dracunculifolia, and affected its biological activity. The major compounds identified were caffeic acid, p-coumaric acid, aromadendrin-4′- methyl ether (AME), isosakuranetin and artepillin C. The IC50 values obtained for CL-lum and CL-luc inhibition by BdE ranged from 8.1-15.8 and 5.8-13.3 μg mL-1, respectively, and correlated positively with caffeic acid concentration. CL-luc inhibition correlated negatively with the concentration of artepillin C, AME, isosakuranetin and total flavonoids. The BdE sample from May/07 inhibited CL-lum and CL-luc the most strongly (IC 50=8.1±1.6 and 5.8±1.0 μg mL-1, respectively), and contained the highest ratio of caffeic acid to the other isolated compounds; so, this ratio could be employed as chemical marker for this biological activity of B. dracunculifolia. Conclusion: The ability of B. dracunculifolia to inhibit the neutrophil ROS generation depends more on the type and ratio of phenolic compounds and flavonoids than on their high absolute concentrations. Together, our results help select the most appropriate plant material for the production of phytotherapic drugs to be used in the treatment of inflammatory diseases mediated by increased neutrophil activation. © 2013 Elsevier Ireland Ltd. All rights reserved. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-25 2022-04-28T18:58:51Z 2022-04-28T18:58:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2013.09.019 Journal of Ethnopharmacology, v. 150, n. 2, p. 655-664, 2013. 0378-8741 1872-7573 http://hdl.handle.net/11449/219971 10.1016/j.jep.2013.09.019 2-s2.0-84887450985 |
url |
http://dx.doi.org/10.1016/j.jep.2013.09.019 http://hdl.handle.net/11449/219971 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 150, n. 2, p. 655-664, 2013. 0378-8741 1872-7573 10.1016/j.jep.2013.09.019 2-s2.0-84887450985 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
655-664 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046735742763008 |