Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572006000400008 http://hdl.handle.net/11449/154099 |
Resumo: | Several pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. This study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (SHR). Thirty-day-old SHR males and Wistar rats were studied. The salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the Lowry method and kinetic method at 405 nm, respectively. Enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. The results were analyzed by the ANOVA or Student's t test (p<0.05). It was noticed that the salivary flow rate (0.026 mL/min/100 g±0.002) and salivary protein concentration (2.26mg/mL±0.14) of SHR offspring were reduced compared to Wistar normotensive offspring (0.036 mL/min/100 g±0.003 and 2.91 mg/mL±0.27, respectively), yet there was no alteration in amylase activity (SHR: 242.4 U/mL±36.9; Wistar: 163.8 U/mL±14.1). Microhardness was lower both in enamel (255.8 KHN±2.6) and dentin (59.9 KHN±0.8) for the SHR teeth compared to the Wistar teeth (enamel: 328.7 KHN±3.3 and dentin: 67.1 KHN±1.0). These results suggest that the SHR offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization. |
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Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)A mineralização dental e a atividade salivar estão reduzidas em filhotes de ratas espontaneamente hipertensas (SHR)Spontaneously hypertensive rat (shr)Tooth mineralizationHypertensionUnitermsHipertensãoRato espontaneamente hipertenso (SHR)SalivaMineralização de denteSeveral pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. This study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (SHR). Thirty-day-old SHR males and Wistar rats were studied. The salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the Lowry method and kinetic method at 405 nm, respectively. Enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. The results were analyzed by the ANOVA or Student's t test (p<0.05). It was noticed that the salivary flow rate (0.026 mL/min/100 g±0.002) and salivary protein concentration (2.26mg/mL±0.14) of SHR offspring were reduced compared to Wistar normotensive offspring (0.036 mL/min/100 g±0.003 and 2.91 mg/mL±0.27, respectively), yet there was no alteration in amylase activity (SHR: 242.4 U/mL±36.9; Wistar: 163.8 U/mL±14.1). Microhardness was lower both in enamel (255.8 KHN±2.6) and dentin (59.9 KHN±0.8) for the SHR teeth compared to the Wistar teeth (enamel: 328.7 KHN±3.3 and dentin: 67.1 KHN±1.0). These results suggest that the SHR offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization.Diversas patologias têm sido diagnosticadas em filhos de mães hipertensas, entretanto alguns estudos que avaliaram as possíveis alterações na saúde oral não são conclusivos. Neste trabalho foram analisadas a atividade das glândulas salivares e a mineralização dental de filhotes de ratas espontaneamente hipertensas (SHR). Ratos machos, com 30 dias de vida, SHR e Wistar, foram estudados. O fluxo salivar foi avaliado pela injeção de pilocarpina e a concentração de proteínas e atividade da amilase na saliva, pelo método de Lowry e método cinético a 405 nm, respectivamente. A mineralização do esmalte e dentina de incisivos inferiores foi quantificada através da medida da microdureza. Os resultados foram analisados pelo teste ANOVA ou t de Student's não pareado (p<0,05). Verificou-se que o fluxo salivar (0,026 mL/min/100 g ± 0,002) e a concentração de proteínas na saliva (2,26 mg/mL ± 0,14) de filhotes SHR estavam reduzidos em comparação com filhotes normotensos Wistar (0,036 mL/min/100 g ± 0,003 e 2,91 mg/mL ± 0,27, respectivamente), porém sem nenhuma alteração da atividade da amilase (SHR: 242,4 U/mL ± 36,9, Wistar: 163,8 U/mL ± 14,1). A microdureza mostrou-se menor tanto no esmalte (255,8 KHN ± 2,6) quanto na dentina (59,9 KHN ± 0,8) dos dentes de SHR em comparação com os de Wistar (esmalte: 328,7 KHN ± 3,3 e dentina: 67,1 KHN ± 1,0 ). Estes resultados sugerem que filhotes SHR seriam mais susceptíveis ao desenvolvimento de patologias que prejudicam a saúde oral, uma vez que apresentaram fluxo e concentração proteica salivares reduzidos e menor mineralização dental.Department of Pediatric Dentistry UNESP - São Paulo State University School of Dentistry, Araçatuba, SPDepartment of Basics Sciences UNESP - Sãu Paulo State University School of Dentistry, Araçatuba, SPFaculdade de Odontologia de Araçatuba UNESP Departamento de Ciências Básicas, Rua José Bonifácio, Cep,: 16015-050, Araçatuba. SPDepartment of Pediatric Dentistry UNESP - São Paulo State University School of Dentistry, Araçatuba, SPDepartment of Basics Sciences UNESP - Sãu Paulo State University School of Dentistry, Araçatuba, SPFaculdade de Odontologia de Araçatuba UNESP Departamento de Ciências Básicas, Rua José Bonifácio, Cep,: 16015-050, Araçatuba. SPJournal Of Applied Oral Science : Revista FobUniversidade Estadual Paulista (Unesp)Elias, Gracieli Prado [UNESP]Santos, Otoniel Antonio Macedo dos [UNESP]Sassaki, Kikue Takebayashi [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Silva, Cristina Antoniali [UNESP]2014-05-27T11:22:04Z2015-12-07T15:29:50Z2014-05-27T11:22:04Z2015-12-07T15:29:50Z2006-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article253-259application/pdfhttp://dx.doi.org/10.1590/S1678-77572006000400008Journal of Applied Oral Science, v. 14, n. 4, p. 253-259, 2006.1678-77571678-7765http://hdl.handle.net/11449/15409910.1590/S1678-77572006000400008S1678-775720060004000082-s2.0-397490996612-s2.0-39749099661.pdf7239471016654133665643353949387919089272PMC43274820000-0002-8159-4853ScopusPubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2024-09-19T17:56:14Zoai:repositorio.unesp.br:11449/154099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T17:56:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) A mineralização dental e a atividade salivar estão reduzidas em filhotes de ratas espontaneamente hipertensas (SHR) |
title |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) |
spellingShingle |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) Elias, Gracieli Prado [UNESP] Spontaneously hypertensive rat (shr) Tooth mineralization Hypertension Uniterms Hipertensão Rato espontaneamente hipertenso (SHR) Saliva Mineralização de dente |
title_short |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) |
title_full |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) |
title_fullStr |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) |
title_full_unstemmed |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) |
title_sort |
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR) |
author |
Elias, Gracieli Prado [UNESP] |
author_facet |
Elias, Gracieli Prado [UNESP] Santos, Otoniel Antonio Macedo dos [UNESP] Sassaki, Kikue Takebayashi [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Silva, Cristina Antoniali [UNESP] |
author_role |
author |
author2 |
Santos, Otoniel Antonio Macedo dos [UNESP] Sassaki, Kikue Takebayashi [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Silva, Cristina Antoniali [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Elias, Gracieli Prado [UNESP] Santos, Otoniel Antonio Macedo dos [UNESP] Sassaki, Kikue Takebayashi [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Silva, Cristina Antoniali [UNESP] |
dc.subject.por.fl_str_mv |
Spontaneously hypertensive rat (shr) Tooth mineralization Hypertension Uniterms Hipertensão Rato espontaneamente hipertenso (SHR) Saliva Mineralização de dente |
topic |
Spontaneously hypertensive rat (shr) Tooth mineralization Hypertension Uniterms Hipertensão Rato espontaneamente hipertenso (SHR) Saliva Mineralização de dente |
description |
Several pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. This study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (SHR). Thirty-day-old SHR males and Wistar rats were studied. The salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the Lowry method and kinetic method at 405 nm, respectively. Enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. The results were analyzed by the ANOVA or Student's t test (p<0.05). It was noticed that the salivary flow rate (0.026 mL/min/100 g±0.002) and salivary protein concentration (2.26mg/mL±0.14) of SHR offspring were reduced compared to Wistar normotensive offspring (0.036 mL/min/100 g±0.003 and 2.91 mg/mL±0.27, respectively), yet there was no alteration in amylase activity (SHR: 242.4 U/mL±36.9; Wistar: 163.8 U/mL±14.1). Microhardness was lower both in enamel (255.8 KHN±2.6) and dentin (59.9 KHN±0.8) for the SHR teeth compared to the Wistar teeth (enamel: 328.7 KHN±3.3 and dentin: 67.1 KHN±1.0). These results suggest that the SHR offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 2014-05-27T11:22:04Z 2014-05-27T11:22:04Z 2015-12-07T15:29:50Z 2015-12-07T15:29:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572006000400008 Journal of Applied Oral Science, v. 14, n. 4, p. 253-259, 2006. 1678-7757 1678-7765 http://hdl.handle.net/11449/154099 10.1590/S1678-77572006000400008 S1678-77572006000400008 2-s2.0-39749099661 2-s2.0-39749099661.pdf 7239471016654133 6656433539493879 19089272 PMC4327482 0000-0002-8159-4853 |
url |
http://dx.doi.org/10.1590/S1678-77572006000400008 http://hdl.handle.net/11449/154099 |
identifier_str_mv |
Journal of Applied Oral Science, v. 14, n. 4, p. 253-259, 2006. 1678-7757 1678-7765 10.1590/S1678-77572006000400008 S1678-77572006000400008 2-s2.0-39749099661 2-s2.0-39749099661.pdf 7239471016654133 6656433539493879 19089272 PMC4327482 0000-0002-8159-4853 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science 1.709 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
253-259 application/pdf |
dc.publisher.none.fl_str_mv |
Journal Of Applied Oral Science : Revista Fob |
publisher.none.fl_str_mv |
Journal Of Applied Oral Science : Revista Fob |
dc.source.none.fl_str_mv |
Scopus PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546407540293632 |