Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)

Detalhes bibliográficos
Autor(a) principal: Elias, Gracieli Prado [UNESP]
Data de Publicação: 2006
Outros Autores: Santos, Otoniel Antonio Macedo dos [UNESP], Sassaki, Kikue Takebayashi [UNESP], Delbem, Alberto Carlos Botazzo [UNESP], Silva, Cristina Antoniali [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-77572006000400008
http://hdl.handle.net/11449/154099
Resumo: Several pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. This study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (SHR). Thirty-day-old SHR males and Wistar rats were studied. The salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the Lowry method and kinetic method at 405 nm, respectively. Enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. The results were analyzed by the ANOVA or Student's t test (p<0.05). It was noticed that the salivary flow rate (0.026 mL/min/100 g±0.002) and salivary protein concentration (2.26mg/mL±0.14) of SHR offspring were reduced compared to Wistar normotensive offspring (0.036 mL/min/100 g±0.003 and 2.91 mg/mL±0.27, respectively), yet there was no alteration in amylase activity (SHR: 242.4 U/mL±36.9; Wistar: 163.8 U/mL±14.1). Microhardness was lower both in enamel (255.8 KHN±2.6) and dentin (59.9 KHN±0.8) for the SHR teeth compared to the Wistar teeth (enamel: 328.7 KHN±3.3 and dentin: 67.1 KHN±1.0). These results suggest that the SHR offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization.
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spelling Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)A mineralização dental e a atividade salivar estão reduzidas em filhotes de ratas espontaneamente hipertensas (SHR)Spontaneously hypertensive rat (shr)Tooth mineralizationHypertensionUnitermsHipertensãoRato espontaneamente hipertenso (SHR)SalivaMineralização de denteSeveral pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. This study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (SHR). Thirty-day-old SHR males and Wistar rats were studied. The salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the Lowry method and kinetic method at 405 nm, respectively. Enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. The results were analyzed by the ANOVA or Student's t test (p<0.05). It was noticed that the salivary flow rate (0.026 mL/min/100 g±0.002) and salivary protein concentration (2.26mg/mL±0.14) of SHR offspring were reduced compared to Wistar normotensive offspring (0.036 mL/min/100 g±0.003 and 2.91 mg/mL±0.27, respectively), yet there was no alteration in amylase activity (SHR: 242.4 U/mL±36.9; Wistar: 163.8 U/mL±14.1). Microhardness was lower both in enamel (255.8 KHN±2.6) and dentin (59.9 KHN±0.8) for the SHR teeth compared to the Wistar teeth (enamel: 328.7 KHN±3.3 and dentin: 67.1 KHN±1.0). These results suggest that the SHR offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization.Diversas patologias têm sido diagnosticadas em filhos de mães hipertensas, entretanto alguns estudos que avaliaram as possíveis alterações na saúde oral não são conclusivos. Neste trabalho foram analisadas a atividade das glândulas salivares e a mineralização dental de filhotes de ratas espontaneamente hipertensas (SHR). Ratos machos, com 30 dias de vida, SHR e Wistar, foram estudados. O fluxo salivar foi avaliado pela injeção de pilocarpina e a concentração de proteínas e atividade da amilase na saliva, pelo método de Lowry e método cinético a 405 nm, respectivamente. A mineralização do esmalte e dentina de incisivos inferiores foi quantificada através da medida da microdureza. Os resultados foram analisados pelo teste ANOVA ou t de Student's não pareado (p<0,05). Verificou-se que o fluxo salivar (0,026 mL/min/100 g ± 0,002) e a concentração de proteínas na saliva (2,26 mg/mL ± 0,14) de filhotes SHR estavam reduzidos em comparação com filhotes normotensos Wistar (0,036 mL/min/100 g ± 0,003 e 2,91 mg/mL ± 0,27, respectivamente), porém sem nenhuma alteração da atividade da amilase (SHR: 242,4 U/mL ± 36,9, Wistar: 163,8 U/mL ± 14,1). A microdureza mostrou-se menor tanto no esmalte (255,8 KHN ± 2,6) quanto na dentina (59,9 KHN ± 0,8) dos dentes de SHR em comparação com os de Wistar (esmalte: 328,7 KHN ± 3,3 e dentina: 67,1 KHN ± 1,0 ). Estes resultados sugerem que filhotes SHR seriam mais susceptíveis ao desenvolvimento de patologias que prejudicam a saúde oral, uma vez que apresentaram fluxo e concentração proteica salivares reduzidos e menor mineralização dental.Department of Pediatric Dentistry UNESP - São Paulo State University School of Dentistry, Araçatuba, SPDepartment of Basics Sciences UNESP - Sãu Paulo State University School of Dentistry, Araçatuba, SPFaculdade de Odontologia de Araçatuba UNESP Departamento de Ciências Básicas, Rua José Bonifácio, Cep,: 16015-050, Araçatuba. SPDepartment of Pediatric Dentistry UNESP - São Paulo State University School of Dentistry, Araçatuba, SPDepartment of Basics Sciences UNESP - Sãu Paulo State University School of Dentistry, Araçatuba, SPFaculdade de Odontologia de Araçatuba UNESP Departamento de Ciências Básicas, Rua José Bonifácio, Cep,: 16015-050, Araçatuba. SPJournal Of Applied Oral Science : Revista FobUniversidade Estadual Paulista (Unesp)Elias, Gracieli Prado [UNESP]Santos, Otoniel Antonio Macedo dos [UNESP]Sassaki, Kikue Takebayashi [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Silva, Cristina Antoniali [UNESP]2014-05-27T11:22:04Z2015-12-07T15:29:50Z2014-05-27T11:22:04Z2015-12-07T15:29:50Z2006-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article253-259application/pdfhttp://dx.doi.org/10.1590/S1678-77572006000400008Journal of Applied Oral Science, v. 14, n. 4, p. 253-259, 2006.1678-77571678-7765http://hdl.handle.net/11449/15409910.1590/S1678-77572006000400008S1678-775720060004000082-s2.0-397490996612-s2.0-39749099661.pdf7239471016654133665643353949387919089272PMC43274820000-0002-8159-4853ScopusPubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2024-09-19T17:56:14Zoai:repositorio.unesp.br:11449/154099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T17:56:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
A mineralização dental e a atividade salivar estão reduzidas em filhotes de ratas espontaneamente hipertensas (SHR)
title Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
spellingShingle Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
Elias, Gracieli Prado [UNESP]
Spontaneously hypertensive rat (shr)
Tooth mineralization
Hypertension
Uniterms
Hipertensão
Rato espontaneamente hipertenso (SHR)
Saliva
Mineralização de dente
title_short Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
title_full Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
title_fullStr Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
title_full_unstemmed Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
title_sort Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
author Elias, Gracieli Prado [UNESP]
author_facet Elias, Gracieli Prado [UNESP]
Santos, Otoniel Antonio Macedo dos [UNESP]
Sassaki, Kikue Takebayashi [UNESP]
Delbem, Alberto Carlos Botazzo [UNESP]
Silva, Cristina Antoniali [UNESP]
author_role author
author2 Santos, Otoniel Antonio Macedo dos [UNESP]
Sassaki, Kikue Takebayashi [UNESP]
Delbem, Alberto Carlos Botazzo [UNESP]
Silva, Cristina Antoniali [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Elias, Gracieli Prado [UNESP]
Santos, Otoniel Antonio Macedo dos [UNESP]
Sassaki, Kikue Takebayashi [UNESP]
Delbem, Alberto Carlos Botazzo [UNESP]
Silva, Cristina Antoniali [UNESP]
dc.subject.por.fl_str_mv Spontaneously hypertensive rat (shr)
Tooth mineralization
Hypertension
Uniterms
Hipertensão
Rato espontaneamente hipertenso (SHR)
Saliva
Mineralização de dente
topic Spontaneously hypertensive rat (shr)
Tooth mineralization
Hypertension
Uniterms
Hipertensão
Rato espontaneamente hipertenso (SHR)
Saliva
Mineralização de dente
description Several pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. This study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (SHR). Thirty-day-old SHR males and Wistar rats were studied. The salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the Lowry method and kinetic method at 405 nm, respectively. Enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. The results were analyzed by the ANOVA or Student's t test (p<0.05). It was noticed that the salivary flow rate (0.026 mL/min/100 g±0.002) and salivary protein concentration (2.26mg/mL±0.14) of SHR offspring were reduced compared to Wistar normotensive offspring (0.036 mL/min/100 g±0.003 and 2.91 mg/mL±0.27, respectively), yet there was no alteration in amylase activity (SHR: 242.4 U/mL±36.9; Wistar: 163.8 U/mL±14.1). Microhardness was lower both in enamel (255.8 KHN±2.6) and dentin (59.9 KHN±0.8) for the SHR teeth compared to the Wistar teeth (enamel: 328.7 KHN±3.3 and dentin: 67.1 KHN±1.0). These results suggest that the SHR offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-01
2014-05-27T11:22:04Z
2014-05-27T11:22:04Z
2015-12-07T15:29:50Z
2015-12-07T15:29:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-77572006000400008
Journal of Applied Oral Science, v. 14, n. 4, p. 253-259, 2006.
1678-7757
1678-7765
http://hdl.handle.net/11449/154099
10.1590/S1678-77572006000400008
S1678-77572006000400008
2-s2.0-39749099661
2-s2.0-39749099661.pdf
7239471016654133
6656433539493879
19089272
PMC4327482
0000-0002-8159-4853
url http://dx.doi.org/10.1590/S1678-77572006000400008
http://hdl.handle.net/11449/154099
identifier_str_mv Journal of Applied Oral Science, v. 14, n. 4, p. 253-259, 2006.
1678-7757
1678-7765
10.1590/S1678-77572006000400008
S1678-77572006000400008
2-s2.0-39749099661
2-s2.0-39749099661.pdf
7239471016654133
6656433539493879
19089272
PMC4327482
0000-0002-8159-4853
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Oral Science
1.709
0,645
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 253-259
application/pdf
dc.publisher.none.fl_str_mv Journal Of Applied Oral Science : Revista Fob
publisher.none.fl_str_mv Journal Of Applied Oral Science : Revista Fob
dc.source.none.fl_str_mv Scopus
PubMed
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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