Bioactivity effects of extracellular matrix proteins on apical papilla cells
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.17632/knzz9hctcd.1 http://hdl.handle.net/11449/218663 |
Resumo: | Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective: This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology: Different concentrations (1, 5, and 10 mu g/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5x10(3) cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; alpha=0.05). Results: We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 mu g/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 mu g/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 mu g/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion: We conclude that FN at 10 mu g/mL concentration exerted the most intense bioactive effects on hAPCs. |
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Bioactivity effects of extracellular matrix proteins on apical papilla cellsGuided tissue regenerationDental pulpFibronectinLamininCollagenPotent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective: This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology: Different concentrations (1, 5, and 10 mu g/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5x10(3) cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; alpha=0.05). Results: We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 mu g/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 mu g/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 mu g/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion: We conclude that FN at 10 mu g/mL concentration exerted the most intense bioactive effects on hAPCs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Fac Odontol Araraquara, Dept Mat Odontol & Protese, UNESP, Araraquara, SP, BrazilUniv Sao Paulo, Fac Odontol Bauru, Dept Dent Endodontia & Mat Odontol, Bauru, SP, BrazilUniv Estadual Paulista, Fac Odontol Araraquara, Dept Morfol & Clin Infantil, UNESP, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, UNESP, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Odontol Araraquara, Dept Mat Odontol & Protese, UNESP, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Odontol Araraquara, Dept Morfol & Clin Infantil, UNESP, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, UNESP, Araraquara, SP, BrazilFAPESP: 2017/14210-8FAPESP: 2017/22739-9CNPq: 302047/2019-0CNPq: 408721/2018-9Univ Sao Paulo Fac Odontologia BauruUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Leite, Maria Luisa [UNESP]Soares, Diana GabrielaAnovazzi, Giovana [UNESP]Wu Mon, Filipe Koon [UNESP]Bordini, Ester Alves Ferreira [UNESP]Hebling, Josimeri [UNESP]De Souza Costa, Carlos Alberto [UNESP]2022-04-28T17:22:21Z2022-04-28T17:22:21Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.17632/knzz9hctcd.1Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 29, 11 p., 2021.1678-7757http://hdl.handle.net/11449/21866310.17632/knzz9hctcd.1WOS:000695653000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2022-04-28T17:22:21Zoai:repositorio.unesp.br:11449/218663Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T17:22:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioactivity effects of extracellular matrix proteins on apical papilla cells |
title |
Bioactivity effects of extracellular matrix proteins on apical papilla cells |
spellingShingle |
Bioactivity effects of extracellular matrix proteins on apical papilla cells Leite, Maria Luisa [UNESP] Guided tissue regeneration Dental pulp Fibronectin Laminin Collagen |
title_short |
Bioactivity effects of extracellular matrix proteins on apical papilla cells |
title_full |
Bioactivity effects of extracellular matrix proteins on apical papilla cells |
title_fullStr |
Bioactivity effects of extracellular matrix proteins on apical papilla cells |
title_full_unstemmed |
Bioactivity effects of extracellular matrix proteins on apical papilla cells |
title_sort |
Bioactivity effects of extracellular matrix proteins on apical papilla cells |
author |
Leite, Maria Luisa [UNESP] |
author_facet |
Leite, Maria Luisa [UNESP] Soares, Diana Gabriela Anovazzi, Giovana [UNESP] Wu Mon, Filipe Koon [UNESP] Bordini, Ester Alves Ferreira [UNESP] Hebling, Josimeri [UNESP] De Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Soares, Diana Gabriela Anovazzi, Giovana [UNESP] Wu Mon, Filipe Koon [UNESP] Bordini, Ester Alves Ferreira [UNESP] Hebling, Josimeri [UNESP] De Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Leite, Maria Luisa [UNESP] Soares, Diana Gabriela Anovazzi, Giovana [UNESP] Wu Mon, Filipe Koon [UNESP] Bordini, Ester Alves Ferreira [UNESP] Hebling, Josimeri [UNESP] De Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
Guided tissue regeneration Dental pulp Fibronectin Laminin Collagen |
topic |
Guided tissue regeneration Dental pulp Fibronectin Laminin Collagen |
description |
Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective: This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology: Different concentrations (1, 5, and 10 mu g/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5x10(3) cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; alpha=0.05). Results: We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 mu g/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 mu g/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 mu g/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion: We conclude that FN at 10 mu g/mL concentration exerted the most intense bioactive effects on hAPCs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T17:22:21Z 2022-04-28T17:22:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.17632/knzz9hctcd.1 Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 29, 11 p., 2021. 1678-7757 http://hdl.handle.net/11449/218663 10.17632/knzz9hctcd.1 WOS:000695653000001 |
url |
http://dx.doi.org/10.17632/knzz9hctcd.1 http://hdl.handle.net/11449/218663 |
identifier_str_mv |
Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 29, 11 p., 2021. 1678-7757 10.17632/knzz9hctcd.1 WOS:000695653000001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046832433004544 |