Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina
Main Author: | |
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Publication Date: | 2016 |
Other Authors: | , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3389/fnana.2016.00082 http://hdl.handle.net/11449/173551 |
Summary: | The zone of transition between the pretectum, derived from prosomere 1, and the thalamus, derived from prosomere 2, is structurally complex and its understanding has been hampered by cytoarchitectural and terminological confusion. Herein, using a battery of complementary morphological approaches, including cytoarchitecture, myeloarchitecture and the expression of molecular markers, we pinpoint the features or combination of features that best characterize each nucleus of the pretectothalamic transitional zone of the rat. Our results reveal useful morphological criteria to identify and delineate, with unprecedented precision, several [mostly auditory] nuclei of the posterior group of the thalamus, namely the pretectothalamic lamina (PTL; formerly known as the posterior limitans nucleus), the medial division of the medial geniculate body (MGBm), the suprageniculate nucleus (SG), and the ethmoid, posterior triangular and posterior nuclei of the thalamus. The PTL is a sparsely-celled and fiber rich flattened nucleus apposed to the lateral surface of the anterior pretectal nucleus (APT) that marks the border between the pretectum and the thalamus; this structure stains selectively with the Wisteria floribunda agglutinin (WFA), and is essentially immunonegative for the calcium binding protein parvalbumin (PV). The MGBm, located medial to the ventral division of the MGB (MGBv), can be unequivocally identified by the large size of many of its neurons, its dark immunostaining for PV, and its rather selective staining for WFA. The SG, which extends for a considerable caudorostral distance and deviates progressively from the MGB, is characterized by its peculiar cytoarchitecture, the paucity of myelinated fibers, and the conspicuous absence of staining for calretinin (CR); indeed, in many CR-stained sections, the SG stands out as a blank spot. Because most of these nuclei are small and show unique anatomical relationships, the information provided in this article will facilitate the interpretation of the results of experimental manipulations aimed at the auditory thalamus and improve the design of future investigations. Moreover, the previously neglected proximity between the MGBm and the caudal region of the scarcely known PTL raises the possibility that certain features or roles traditionally attributed to the MGBm may actually belong to the PTL. |
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Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic laminaAnterior pretectal nucleusAuditory thalamusEthmoid nucleusMedial geniculate bodyPosterior limitans nucleus of the thalamusRatSuprageniculate nucleusWisteria floribunda agglutinin (WFA)The zone of transition between the pretectum, derived from prosomere 1, and the thalamus, derived from prosomere 2, is structurally complex and its understanding has been hampered by cytoarchitectural and terminological confusion. Herein, using a battery of complementary morphological approaches, including cytoarchitecture, myeloarchitecture and the expression of molecular markers, we pinpoint the features or combination of features that best characterize each nucleus of the pretectothalamic transitional zone of the rat. Our results reveal useful morphological criteria to identify and delineate, with unprecedented precision, several [mostly auditory] nuclei of the posterior group of the thalamus, namely the pretectothalamic lamina (PTL; formerly known as the posterior limitans nucleus), the medial division of the medial geniculate body (MGBm), the suprageniculate nucleus (SG), and the ethmoid, posterior triangular and posterior nuclei of the thalamus. The PTL is a sparsely-celled and fiber rich flattened nucleus apposed to the lateral surface of the anterior pretectal nucleus (APT) that marks the border between the pretectum and the thalamus; this structure stains selectively with the Wisteria floribunda agglutinin (WFA), and is essentially immunonegative for the calcium binding protein parvalbumin (PV). The MGBm, located medial to the ventral division of the MGB (MGBv), can be unequivocally identified by the large size of many of its neurons, its dark immunostaining for PV, and its rather selective staining for WFA. The SG, which extends for a considerable caudorostral distance and deviates progressively from the MGB, is characterized by its peculiar cytoarchitecture, the paucity of myelinated fibers, and the conspicuous absence of staining for calretinin (CR); indeed, in many CR-stained sections, the SG stands out as a blank spot. Because most of these nuclei are small and show unique anatomical relationships, the information provided in this article will facilitate the interpretation of the results of experimental manipulations aimed at the auditory thalamus and improve the design of future investigations. Moreover, the previously neglected proximity between the MGBm and the caudal region of the scarcely known PTL raises the possibility that certain features or roles traditionally attributed to the MGBm may actually belong to the PTL.Neuroscience Institute of Castilla y León (INCyL) University of SalamancaDepartment of Cell Biology and Pathology Medical School University of SalamancaDepartment of Anatomy Institute of Biosciences of Botucatu UNESP—Universidade Estadual PaulistaDepartment of Otolaryngology–Head and Neck Surgery Sensory Neuroscience Research Center West Virginia UniversityInstitute of Biomedical Research of Salamanca (IBSAL) University of SalamancaDepartment of Anatomy Institute of Biosciences of Botucatu UNESP—Universidade Estadual PaulistaUniversity of SalamancaUniversidade Estadual Paulista (Unesp)West Virginia UniversityMárquez-Legorreta, EmmanuelHorta-Júnior, José de Anchieta C. [UNESP]Berrebi, Albert S.Saldaña, Enrique2018-12-11T17:06:14Z2018-12-11T17:06:14Z2016-08-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3389/fnana.2016.00082Frontiers in Neuroanatomy, v. 10, n. AUG, 2016.1662-5129http://hdl.handle.net/11449/17355110.3389/fnana.2016.000822-s2.0-849898290842-s2.0-84989829084.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Neuroanatomy1,999info:eu-repo/semantics/openAccess2023-10-29T06:10:07Zoai:repositorio.unesp.br:11449/173551Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-29T06:10:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina |
title |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina |
spellingShingle |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina Márquez-Legorreta, Emmanuel Anterior pretectal nucleus Auditory thalamus Ethmoid nucleus Medial geniculate body Posterior limitans nucleus of the thalamus Rat Suprageniculate nucleus Wisteria floribunda agglutinin (WFA) |
title_short |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina |
title_full |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina |
title_fullStr |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina |
title_full_unstemmed |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina |
title_sort |
Organization of the zone of transition between the pretectum and the thalamus, with emphasis on the pretectothalamic lamina |
author |
Márquez-Legorreta, Emmanuel |
author_facet |
Márquez-Legorreta, Emmanuel Horta-Júnior, José de Anchieta C. [UNESP] Berrebi, Albert S. Saldaña, Enrique |
author_role |
author |
author2 |
Horta-Júnior, José de Anchieta C. [UNESP] Berrebi, Albert S. Saldaña, Enrique |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
University of Salamanca Universidade Estadual Paulista (Unesp) West Virginia University |
dc.contributor.author.fl_str_mv |
Márquez-Legorreta, Emmanuel Horta-Júnior, José de Anchieta C. [UNESP] Berrebi, Albert S. Saldaña, Enrique |
dc.subject.por.fl_str_mv |
Anterior pretectal nucleus Auditory thalamus Ethmoid nucleus Medial geniculate body Posterior limitans nucleus of the thalamus Rat Suprageniculate nucleus Wisteria floribunda agglutinin (WFA) |
topic |
Anterior pretectal nucleus Auditory thalamus Ethmoid nucleus Medial geniculate body Posterior limitans nucleus of the thalamus Rat Suprageniculate nucleus Wisteria floribunda agglutinin (WFA) |
description |
The zone of transition between the pretectum, derived from prosomere 1, and the thalamus, derived from prosomere 2, is structurally complex and its understanding has been hampered by cytoarchitectural and terminological confusion. Herein, using a battery of complementary morphological approaches, including cytoarchitecture, myeloarchitecture and the expression of molecular markers, we pinpoint the features or combination of features that best characterize each nucleus of the pretectothalamic transitional zone of the rat. Our results reveal useful morphological criteria to identify and delineate, with unprecedented precision, several [mostly auditory] nuclei of the posterior group of the thalamus, namely the pretectothalamic lamina (PTL; formerly known as the posterior limitans nucleus), the medial division of the medial geniculate body (MGBm), the suprageniculate nucleus (SG), and the ethmoid, posterior triangular and posterior nuclei of the thalamus. The PTL is a sparsely-celled and fiber rich flattened nucleus apposed to the lateral surface of the anterior pretectal nucleus (APT) that marks the border between the pretectum and the thalamus; this structure stains selectively with the Wisteria floribunda agglutinin (WFA), and is essentially immunonegative for the calcium binding protein parvalbumin (PV). The MGBm, located medial to the ventral division of the MGB (MGBv), can be unequivocally identified by the large size of many of its neurons, its dark immunostaining for PV, and its rather selective staining for WFA. The SG, which extends for a considerable caudorostral distance and deviates progressively from the MGB, is characterized by its peculiar cytoarchitecture, the paucity of myelinated fibers, and the conspicuous absence of staining for calretinin (CR); indeed, in many CR-stained sections, the SG stands out as a blank spot. Because most of these nuclei are small and show unique anatomical relationships, the information provided in this article will facilitate the interpretation of the results of experimental manipulations aimed at the auditory thalamus and improve the design of future investigations. Moreover, the previously neglected proximity between the MGBm and the caudal region of the scarcely known PTL raises the possibility that certain features or roles traditionally attributed to the MGBm may actually belong to the PTL. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-11 2018-12-11T17:06:14Z 2018-12-11T17:06:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fnana.2016.00082 Frontiers in Neuroanatomy, v. 10, n. AUG, 2016. 1662-5129 http://hdl.handle.net/11449/173551 10.3389/fnana.2016.00082 2-s2.0-84989829084 2-s2.0-84989829084.pdf |
url |
http://dx.doi.org/10.3389/fnana.2016.00082 http://hdl.handle.net/11449/173551 |
identifier_str_mv |
Frontiers in Neuroanatomy, v. 10, n. AUG, 2016. 1662-5129 10.3389/fnana.2016.00082 2-s2.0-84989829084 2-s2.0-84989829084.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Neuroanatomy 1,999 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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