Detecção de microrganismos de infecções bucais: perspectivas e cuidados a serem seguidos

• Autor(a) principal: Jardim Júnior, Elerson Gaetti [UNESP]
• Data de Publicação: 2011
• Outros Autores: Lins, Samira Ambar, Jardim, Ellen Cristina Gaetti [UNESP], Ramos, Marcelle Marie Buso [UNESP], Aguiar, Rosangela Conceição Miotti de Souza [UNESP], Ranieri, Robson Varlei [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://seer.funecsantafe.edu.br/index.php?journal=rfc&page=article&op=view&path%5B%5D=28; http://hdl.handle.net/11449/133528
• topic: Diagnosis; Antimicrobials; Head and neck infections; Diagnóstico; Antimicrobianos; Infecções de cabeça e pescoço

The diagnosis of head and neck infections constitutes relevant step in their treatment. However, in spite of the fact that most of diseases in head and neck region are infectious in nature, several reasons collaborated for dentists do not ask laboratory tests in order to help clinical diagnosis. By mean of this review literature, based on research articles about the newest and most reliable methods of diagnosis for clinical laboratories, the authors discuss the advantages and disadvantages of each selected method and the relevant aspects in transportation of the specimens to the laboratory. Saliva, biofilm, pus, and blood are the most frequent specimens for microbial diagnosis, being that the most used methods are culture and those based on detection of deoxyribonucleic acid by polymerase chain reaction method. Whereas, the culture depends on cellular viability, and has reduced sensitivity, as well as needs favorable conditions in the sample collection and transportation, PCR shows high sensitivity and specificity, but it does not allow the determination of antibiogram, what reduces its usefulness. In addition, few laboratories possess conditions to perform cultivation of obligate anaerobes or have experience in the molecular detection of these microorganisms.