Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.placenta.2021.12.003 http://hdl.handle.net/11449/230028 |
Resumo: | Preeclampsia (PE) is classified into late-onset (LOPE) or early-onset (EOPE) according to gestational age of onset (≥34 or <34 weeks, respectively), and into preterm and term (delivery at <37 or ≥37 weeks, respectively). An imbalanced expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) impairs proper placentation in PE, and DNA methylation (DNAm) may affect their expression. We performed comprehensive analyses of DNAm and TIMP3 expression in placentas from PE reclassified into EOPE, LOPE, and term PE. We identified significant differentially methylated probes at the TIMP3 promoter in PE (28), EOPE (38), LOPE (20), and term PE (4) compared to controls, and in EOPE vs. LOPE (8). Moreover, we found a hypomethylation >70% in all groups (except EOPE vs. LOPE) and an increased TIMP3 expression in corresponding placental samples from PE, EOPE and LOPE compared to controls (p<0.05). Our findings highlight the role of DNAm of the TIMP3 promoter region regarding an epigenetic mechanism in PE. |
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Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsiaDNA methylationEarly-onset pre-eclampsiaEpigenomicsLate-onset pre-eclampsiaPre-eclampsiaTissue inhibitor of Metalloproteinase-3Preeclampsia (PE) is classified into late-onset (LOPE) or early-onset (EOPE) according to gestational age of onset (≥34 or <34 weeks, respectively), and into preterm and term (delivery at <37 or ≥37 weeks, respectively). An imbalanced expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) impairs proper placentation in PE, and DNA methylation (DNAm) may affect their expression. We performed comprehensive analyses of DNAm and TIMP3 expression in placentas from PE reclassified into EOPE, LOPE, and term PE. We identified significant differentially methylated probes at the TIMP3 promoter in PE (28), EOPE (38), LOPE (20), and term PE (4) compared to controls, and in EOPE vs. LOPE (8). Moreover, we found a hypomethylation >70% in all groups (except EOPE vs. LOPE) and an increased TIMP3 expression in corresponding placental samples from PE, EOPE and LOPE compared to controls (p<0.05). Our findings highlight the role of DNAm of the TIMP3 promoter region regarding an epigenetic mechanism in PE.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Genetics Graduate Program Institute of Biological Sciences Federal University of Minas GeraisBiochemistry and Immunology Graduate Program Institute of Biological Sciences Federal University of Minas GeraisDepartment of Biophysics and Pharmacology Institute of Biosciences Universidade Estadual Paulista, BotucatuDepartment of Genetics Ecology and Evolution Institute of Biological Sciences Federal University of Minas GeraisDepartment of Biophysics and Pharmacology Institute of Biosciences Universidade Estadual Paulista, BotucatuCNPq: #312599/2019–6CAPES: 001CAPES: 2019/07230-8CAPES: APQ-01960-18Universidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (UNESP)Cruz, Juliana de O.Conceição, Izabela M.C.A.Sandrim, Valeria C. [UNESP]Luizon, Marcelo R.2022-04-29T08:37:16Z2022-04-29T08:37:16Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article118-121http://dx.doi.org/10.1016/j.placenta.2021.12.003Placenta, v. 117, p. 118-121.1532-31020143-4004http://hdl.handle.net/11449/23002810.1016/j.placenta.2021.12.0032-s2.0-85120891170Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlacentainfo:eu-repo/semantics/openAccess2022-04-29T08:37:16Zoai:repositorio.unesp.br:11449/230028Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:48:24.956018Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia |
title |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia |
spellingShingle |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia Cruz, Juliana de O. DNA methylation Early-onset pre-eclampsia Epigenomics Late-onset pre-eclampsia Pre-eclampsia Tissue inhibitor of Metalloproteinase-3 |
title_short |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia |
title_full |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia |
title_fullStr |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia |
title_full_unstemmed |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia |
title_sort |
Comprehensive analyses of DNA methylation of the TIMP3 promoter in placentas from early-onset and late-onset preeclampsia |
author |
Cruz, Juliana de O. |
author_facet |
Cruz, Juliana de O. Conceição, Izabela M.C.A. Sandrim, Valeria C. [UNESP] Luizon, Marcelo R. |
author_role |
author |
author2 |
Conceição, Izabela M.C.A. Sandrim, Valeria C. [UNESP] Luizon, Marcelo R. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Cruz, Juliana de O. Conceição, Izabela M.C.A. Sandrim, Valeria C. [UNESP] Luizon, Marcelo R. |
dc.subject.por.fl_str_mv |
DNA methylation Early-onset pre-eclampsia Epigenomics Late-onset pre-eclampsia Pre-eclampsia Tissue inhibitor of Metalloproteinase-3 |
topic |
DNA methylation Early-onset pre-eclampsia Epigenomics Late-onset pre-eclampsia Pre-eclampsia Tissue inhibitor of Metalloproteinase-3 |
description |
Preeclampsia (PE) is classified into late-onset (LOPE) or early-onset (EOPE) according to gestational age of onset (≥34 or <34 weeks, respectively), and into preterm and term (delivery at <37 or ≥37 weeks, respectively). An imbalanced expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) impairs proper placentation in PE, and DNA methylation (DNAm) may affect their expression. We performed comprehensive analyses of DNAm and TIMP3 expression in placentas from PE reclassified into EOPE, LOPE, and term PE. We identified significant differentially methylated probes at the TIMP3 promoter in PE (28), EOPE (38), LOPE (20), and term PE (4) compared to controls, and in EOPE vs. LOPE (8). Moreover, we found a hypomethylation >70% in all groups (except EOPE vs. LOPE) and an increased TIMP3 expression in corresponding placental samples from PE, EOPE and LOPE compared to controls (p<0.05). Our findings highlight the role of DNAm of the TIMP3 promoter region regarding an epigenetic mechanism in PE. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:37:16Z 2022-04-29T08:37:16Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.placenta.2021.12.003 Placenta, v. 117, p. 118-121. 1532-3102 0143-4004 http://hdl.handle.net/11449/230028 10.1016/j.placenta.2021.12.003 2-s2.0-85120891170 |
url |
http://dx.doi.org/10.1016/j.placenta.2021.12.003 http://hdl.handle.net/11449/230028 |
identifier_str_mv |
Placenta, v. 117, p. 118-121. 1532-3102 0143-4004 10.1016/j.placenta.2021.12.003 2-s2.0-85120891170 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Placenta |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
118-121 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128278344499200 |