Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria
Autor(a) principal: | |
---|---|
Data de Publicação: | 1999 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1161/01.HYP.34.4.802 http://hdl.handle.net/11449/65847 |
Resumo: | The long-term administration of nitric oxide synthesis inhibitors induces arterial hypertension accompanied by left ventricular hypertrophy and myocardial ischemic lesions. Because the enhancement of sympathetic drive has been implicated in these phenomena, the current study was performed to determine the potency of β-adrenoceptor agonists and muscarinic agonists on the spontaneous rate of isolated right atria from rats given long-term treatment with the nitric oxide inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME). Atrial lesions induced by long-term treatment with L-NAME were also evaluated. Long-term L-NAME treatment caused a time-dependent, significant (P<0.05) increase in tail-cuff pressure compared with control animals. Our results showed that the potency of isoproterenol, norepinephrine, carbachol, and pilocarpine in isolated right atria from rats given long-term treatment with L-NAME for 7, 15, 30, and 60 days was not affected as compared with control animals. Addition of L-NAME in vitro (100 μmol/L) affected neither basal rate nor chronotropic response for isoproterenol and norepinephrine in rat heart. Stereological analysis of the right atria at 15 and 30 days revealed a significant increase on amount of fibrous tissues in L-NAME- treated groups (27±2.3% and 28±1.3% for 15 and 30 days, respectively; P<0.05) as compared with the control group (22±1.1%). Our results indicate that nitric oxide does not to interfere with β-adrenoceptor-mediated and muscarinic receptor-mediated chronotropic responses. |
id |
UNSP_530ec2ea462f9161dd534f4c755ba2b5 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/65847 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atriaAdrenergic receptor agonistsBlood pressureNitric oxideReceptors, adrenergic, betaReceptors, muscariniccarbacholisoprenalinen(g) nitroarginine methyl esternitric oxidenoradrenalinpilocarpineadrenergic systemanimal experimentanimal tissueblood pressure measurementchronotropismconcentration responsecontrolled studydrug potencyheart right atriumnonhumanpriority journalratAnimalsBlood PressureEnzyme InhibitorsHeart AtriaMaleMyocardial ContractionNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthaseRatsRats, WistarSympathetic Nervous SystemVentricular Function, RightThe long-term administration of nitric oxide synthesis inhibitors induces arterial hypertension accompanied by left ventricular hypertrophy and myocardial ischemic lesions. Because the enhancement of sympathetic drive has been implicated in these phenomena, the current study was performed to determine the potency of β-adrenoceptor agonists and muscarinic agonists on the spontaneous rate of isolated right atria from rats given long-term treatment with the nitric oxide inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME). Atrial lesions induced by long-term treatment with L-NAME were also evaluated. Long-term L-NAME treatment caused a time-dependent, significant (P<0.05) increase in tail-cuff pressure compared with control animals. Our results showed that the potency of isoproterenol, norepinephrine, carbachol, and pilocarpine in isolated right atria from rats given long-term treatment with L-NAME for 7, 15, 30, and 60 days was not affected as compared with control animals. Addition of L-NAME in vitro (100 μmol/L) affected neither basal rate nor chronotropic response for isoproterenol and norepinephrine in rat heart. Stereological analysis of the right atria at 15 and 30 days revealed a significant increase on amount of fibrous tissues in L-NAME- treated groups (27±2.3% and 28±1.3% for 15 and 30 days, respectively; P<0.05) as compared with the control group (22±1.1%). Our results indicate that nitric oxide does not to interfere with β-adrenoceptor-mediated and muscarinic receptor-mediated chronotropic responses.Department of Pharmacology Faculty of Medical Sciences UNICAMP, CampinasDept. of Histology and Embryology Biology Insitute UNICAMP, CampinasDepartment of Physical Education Biosciences Institute UNESP, Rio ClaroDepartment of Pharmacology Louisiana Stt. Univ. Medical Center, New Orleans, LADepartment of Physical Education Bioscience Institute UNESP, Av 24A no 1515, CEP 13506-900, Rio Claro (SP)Department of Physical Education Biosciences Institute UNESP, Rio ClaroDepartment of Physical Education Bioscience Institute UNESP, Av 24A no 1515, CEP 13506-900, Rio Claro (SP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Louisiana Stt. Univ. Medical CenterRiado, Sonia R.Zanesco, Angelina [UNESP]Barker, Louis AllenDe Luca, Iara M.S.Antunes, EdsonDe Nucci, Gilberto2014-05-27T11:19:46Z2014-05-27T11:19:46Z1999-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article802-807http://dx.doi.org/10.1161/01.HYP.34.4.802Hypertension, v. 34, n. 4 II, p. 802-807, 1999.0194-911Xhttp://hdl.handle.net/11449/6584710.1161/01.HYP.34.4.802WOS:0000834865000182-s2.0-00327591694472007237545596Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHypertension6.823info:eu-repo/semantics/openAccess2021-10-22T20:49:00Zoai:repositorio.unesp.br:11449/65847Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria |
title |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria |
spellingShingle |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria Riado, Sonia R. Adrenergic receptor agonists Blood pressure Nitric oxide Receptors, adrenergic, beta Receptors, muscarinic carbachol isoprenaline n(g) nitroarginine methyl ester nitric oxide noradrenalin pilocarpine adrenergic system animal experiment animal tissue blood pressure measurement chronotropism concentration response controlled study drug potency heart right atrium nonhuman priority journal rat Animals Blood Pressure Enzyme Inhibitors Heart Atria Male Myocardial Contraction NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Rats Rats, Wistar Sympathetic Nervous System Ventricular Function, Right |
title_short |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria |
title_full |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria |
title_fullStr |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria |
title_full_unstemmed |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria |
title_sort |
Long-term nitric oxide inhibition and chronotropic responses in rat isolated right atria |
author |
Riado, Sonia R. |
author_facet |
Riado, Sonia R. Zanesco, Angelina [UNESP] Barker, Louis Allen De Luca, Iara M.S. Antunes, Edson De Nucci, Gilberto |
author_role |
author |
author2 |
Zanesco, Angelina [UNESP] Barker, Louis Allen De Luca, Iara M.S. Antunes, Edson De Nucci, Gilberto |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Louisiana Stt. Univ. Medical Center |
dc.contributor.author.fl_str_mv |
Riado, Sonia R. Zanesco, Angelina [UNESP] Barker, Louis Allen De Luca, Iara M.S. Antunes, Edson De Nucci, Gilberto |
dc.subject.por.fl_str_mv |
Adrenergic receptor agonists Blood pressure Nitric oxide Receptors, adrenergic, beta Receptors, muscarinic carbachol isoprenaline n(g) nitroarginine methyl ester nitric oxide noradrenalin pilocarpine adrenergic system animal experiment animal tissue blood pressure measurement chronotropism concentration response controlled study drug potency heart right atrium nonhuman priority journal rat Animals Blood Pressure Enzyme Inhibitors Heart Atria Male Myocardial Contraction NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Rats Rats, Wistar Sympathetic Nervous System Ventricular Function, Right |
topic |
Adrenergic receptor agonists Blood pressure Nitric oxide Receptors, adrenergic, beta Receptors, muscarinic carbachol isoprenaline n(g) nitroarginine methyl ester nitric oxide noradrenalin pilocarpine adrenergic system animal experiment animal tissue blood pressure measurement chronotropism concentration response controlled study drug potency heart right atrium nonhuman priority journal rat Animals Blood Pressure Enzyme Inhibitors Heart Atria Male Myocardial Contraction NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Rats Rats, Wistar Sympathetic Nervous System Ventricular Function, Right |
description |
The long-term administration of nitric oxide synthesis inhibitors induces arterial hypertension accompanied by left ventricular hypertrophy and myocardial ischemic lesions. Because the enhancement of sympathetic drive has been implicated in these phenomena, the current study was performed to determine the potency of β-adrenoceptor agonists and muscarinic agonists on the spontaneous rate of isolated right atria from rats given long-term treatment with the nitric oxide inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME). Atrial lesions induced by long-term treatment with L-NAME were also evaluated. Long-term L-NAME treatment caused a time-dependent, significant (P<0.05) increase in tail-cuff pressure compared with control animals. Our results showed that the potency of isoproterenol, norepinephrine, carbachol, and pilocarpine in isolated right atria from rats given long-term treatment with L-NAME for 7, 15, 30, and 60 days was not affected as compared with control animals. Addition of L-NAME in vitro (100 μmol/L) affected neither basal rate nor chronotropic response for isoproterenol and norepinephrine in rat heart. Stereological analysis of the right atria at 15 and 30 days revealed a significant increase on amount of fibrous tissues in L-NAME- treated groups (27±2.3% and 28±1.3% for 15 and 30 days, respectively; P<0.05) as compared with the control group (22±1.1%). Our results indicate that nitric oxide does not to interfere with β-adrenoceptor-mediated and muscarinic receptor-mediated chronotropic responses. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-10-01 2014-05-27T11:19:46Z 2014-05-27T11:19:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1161/01.HYP.34.4.802 Hypertension, v. 34, n. 4 II, p. 802-807, 1999. 0194-911X http://hdl.handle.net/11449/65847 10.1161/01.HYP.34.4.802 WOS:000083486500018 2-s2.0-0032759169 4472007237545596 |
url |
http://dx.doi.org/10.1161/01.HYP.34.4.802 http://hdl.handle.net/11449/65847 |
identifier_str_mv |
Hypertension, v. 34, n. 4 II, p. 802-807, 1999. 0194-911X 10.1161/01.HYP.34.4.802 WOS:000083486500018 2-s2.0-0032759169 4472007237545596 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hypertension 6.823 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
802-807 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790042936049664 |