Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms21124295 http://hdl.handle.net/11449/200628 |
Resumo: | Unlike seed plants, ferns leaves are considered to be structures with delayed determinacy, with a leaf apical meristem similar to the shoot apical meristems. To better understand the meristematic organization during leaf development and determinacy control, we analyzed the cell divisions and expression of Class I KNOX genes in Mickelia scandens, a fern that produces larger leaves with more pinnae in its climbing form than in its terrestrial form. We performed anatomical, in situ hybridization, and qRT-PCR experiments with histone H4 (cell division marker) and Class I KNOX genes. We found that Class I KNOX genes are expressed in shoot apical meristems, leaf apical meristems, and pinnae primordia. During early development, cell divisions occur in the most distal regions of the analyzed structures, including pinnae, and are not restricted to apical cells. Fern leaves and pinnae bear apical meristems that may partially act as indeterminate shoots, supporting the hypothesis of homology between shoots and leaves. Class I KNOX expression is correlated with indeterminacy in the apex and leaf of ferns, suggesting a conserved function for these genes in euphyllophytes with compound leaves. |
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Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae)Apical meristemsClass I KNOX genesCompound leavesDeterminacyDryopteridaceaeFernsLeaf developmentPinna developmentShoot developmentUnlike seed plants, ferns leaves are considered to be structures with delayed determinacy, with a leaf apical meristem similar to the shoot apical meristems. To better understand the meristematic organization during leaf development and determinacy control, we analyzed the cell divisions and expression of Class I KNOX genes in Mickelia scandens, a fern that produces larger leaves with more pinnae in its climbing form than in its terrestrial form. We performed anatomical, in situ hybridization, and qRT-PCR experiments with histone H4 (cell division marker) and Class I KNOX genes. We found that Class I KNOX genes are expressed in shoot apical meristems, leaf apical meristems, and pinnae primordia. During early development, cell divisions occur in the most distal regions of the analyzed structures, including pinnae, and are not restricted to apical cells. Fern leaves and pinnae bear apical meristems that may partially act as indeterminate shoots, supporting the hypothesis of homology between shoots and leaves. Class I KNOX expression is correlated with indeterminacy in the apex and leaf of ferns, suggesting a conserved function for these genes in euphyllophytes with compound leaves.New York Botanical GardenFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Science FoundationInstituto de Botânica, Av. Miguel Estéfano 3687Instituto de Biociências Universidade de São Paulo, Rua do Matão 277Botanical Research Institute of Texas, 1700 University DriveUNESP IBILCE Depto. de Zoologia e Botânica, Rua Cristóvão Colombo, 2265The New York Botanical Garden, 2900 Southern BlvdUNESP IBILCE Depto. de Zoologia e Botânica, Rua Cristóvão Colombo, 2265FAPESP: 2013/26191-7FAPESP: 2015/15158-4FAPESP: 2015/15920-3CNPq: 300810/2016-4CNPq: 301157/2010-3CNPq: 303962/2019-4National Science Foundation: DEB-1020443Instituto de BotânicaUniversidade de São Paulo (USP)Botanical Research Institute of TexasUniversidade Estadual Paulista (Unesp)The New York Botanical GardenCruz, RafaelMelo-De-pinna, Gladys F. A.Vasco, AlejandraPrado, Jefferson [UNESP]Ambrose, Barbara A.2020-12-12T02:11:43Z2020-12-12T02:11:43Z2020-06-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-13http://dx.doi.org/10.3390/ijms21124295International Journal of Molecular Sciences, v. 21, n. 12, p. 1-13, 2020.1422-00671661-6596http://hdl.handle.net/11449/20062810.3390/ijms211242952-s2.0-85086753291Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T14:48:25Zoai:repositorio.unesp.br:11449/200628Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:33:33.407853Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) |
title |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) |
spellingShingle |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) Cruz, Rafael Apical meristems Class I KNOX genes Compound leaves Determinacy Dryopteridaceae Ferns Leaf development Pinna development Shoot development |
title_short |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) |
title_full |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) |
title_fullStr |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) |
title_full_unstemmed |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) |
title_sort |
Class I KNOX is related to determinacy during the leaf development of the fern Mickelia scandens (Dryopteridaceae) |
author |
Cruz, Rafael |
author_facet |
Cruz, Rafael Melo-De-pinna, Gladys F. A. Vasco, Alejandra Prado, Jefferson [UNESP] Ambrose, Barbara A. |
author_role |
author |
author2 |
Melo-De-pinna, Gladys F. A. Vasco, Alejandra Prado, Jefferson [UNESP] Ambrose, Barbara A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Botânica Universidade de São Paulo (USP) Botanical Research Institute of Texas Universidade Estadual Paulista (Unesp) The New York Botanical Garden |
dc.contributor.author.fl_str_mv |
Cruz, Rafael Melo-De-pinna, Gladys F. A. Vasco, Alejandra Prado, Jefferson [UNESP] Ambrose, Barbara A. |
dc.subject.por.fl_str_mv |
Apical meristems Class I KNOX genes Compound leaves Determinacy Dryopteridaceae Ferns Leaf development Pinna development Shoot development |
topic |
Apical meristems Class I KNOX genes Compound leaves Determinacy Dryopteridaceae Ferns Leaf development Pinna development Shoot development |
description |
Unlike seed plants, ferns leaves are considered to be structures with delayed determinacy, with a leaf apical meristem similar to the shoot apical meristems. To better understand the meristematic organization during leaf development and determinacy control, we analyzed the cell divisions and expression of Class I KNOX genes in Mickelia scandens, a fern that produces larger leaves with more pinnae in its climbing form than in its terrestrial form. We performed anatomical, in situ hybridization, and qRT-PCR experiments with histone H4 (cell division marker) and Class I KNOX genes. We found that Class I KNOX genes are expressed in shoot apical meristems, leaf apical meristems, and pinnae primordia. During early development, cell divisions occur in the most distal regions of the analyzed structures, including pinnae, and are not restricted to apical cells. Fern leaves and pinnae bear apical meristems that may partially act as indeterminate shoots, supporting the hypothesis of homology between shoots and leaves. Class I KNOX expression is correlated with indeterminacy in the apex and leaf of ferns, suggesting a conserved function for these genes in euphyllophytes with compound leaves. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:11:43Z 2020-12-12T02:11:43Z 2020-06-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms21124295 International Journal of Molecular Sciences, v. 21, n. 12, p. 1-13, 2020. 1422-0067 1661-6596 http://hdl.handle.net/11449/200628 10.3390/ijms21124295 2-s2.0-85086753291 |
url |
http://dx.doi.org/10.3390/ijms21124295 http://hdl.handle.net/11449/200628 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 21, n. 12, p. 1-13, 2020. 1422-0067 1661-6596 10.3390/ijms21124295 2-s2.0-85086753291 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-13 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129437554704384 |